Simultaneous determination of eight active components in Houttuynia cordata injection and its quality control in productive process

2011 ◽  
Vol 34 (21) ◽  
pp. 3053-3060 ◽  
Author(s):  
Wei Ji ◽  
Kaishun Bi ◽  
Qianqian Chen ◽  
Lingyan Jiang ◽  
Ke Liang ◽  
...  
Keyword(s):  
Quality Control ◽  
Simultaneous Determination ◽  
Active Components ◽  
Houttuynia Cordata ◽  
Productive Process

Simultaneous Determination of Eight Potential Q-Markers in Zishen Tongguan Capsules Based on UHPLC-MS/MS

2020 ◽  
Vol 17 (1) ◽  
pp. 47-56
Author(s):  
Shun Liu ◽  
Xun Wang ◽  
Kaiping Zou ◽  
Wei Liu ◽  
Cunyu Li ◽  
...  
Keyword(s):  
Quality Control ◽  
Chinese Medicine ◽  
Simultaneous Determination ◽  
High Performance ◽  
Multiple Reaction Monitoring ◽  
Gradient Elution ◽  
Quality Markers ◽  
High Repeatability ◽  
Comprehensive Study

Background: Zishen Tongguan (ZSTG) capsules were prepared at the Affiliated Hospital of Nanjing University of Chinese Medicine and have been proven to be clinically effective for treating pyelonephritis and benign prostatic hyperplasia. However, the quality standards are not ideal; a comprehensive study of the “quality markers” (Q-markers), the chemicals inherent in traditional Chinese medicine and its preparations, has not been carried out. Experimental Methods: In this paper, a sensitive and specific ultra-high-performance liquid chromatographictandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous determination of eight potential Q-markers of ZSTG, including timosaponin A3, berberine, jatrorrhizine, phellodendrine, palmatine, mangiferin, neomangiferin, and timosaponin BII. A Kromasil 100-3.5 C18 column was used with a mobile phase of 0.2% formic acid with acetonitrile, and gradient elution at a flow rate of 0.2 mL/min was achieved in 13 minutes and used for separation. Detection was performed in positive/negative mode with multiple reaction monitoring (MRM). Results: The analytical method was validated in terms of the sensitivity, linearity, accuracy, precision, repeatability, stability and recovery. The method established here was successfully applied to study the potential Q-markers in 8 batches of commercial samples, which demonstrated its use in improving the quality control of ZSTG. Conclusion: The developed method had high repeatability and accuracy and was suitable for the simultaneous analysis of multiple Q-markers, which may provide a new basis for the comprehensive assessment and overall quality control of ZSTG.

Improved LC Method for the Simultaneous Determination of Five Active Components in Danshen and Its Preparations

2009 ◽  
Vol 69 (5-6) ◽  
pp. 543-548 ◽  
Author(s):  
Xiao-Bing Li ◽  
Xiao-Mei Xie ◽  
Wei-Zhong Pei ◽  
Jia-Kuan Chen ◽  
Yan Song ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Active Components

scholarly journals Simultaneous Determination of Four Major Constituents of Semen Vaccariae Using HPLC-DAD

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Haijiang Zhang ◽  
Wei Yao ◽  
Yunyun Chen ◽  
Peipei He ◽  
Yao Chen ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Chromatographic Separation ◽  
Gradient Elution ◽  
Hplc Method ◽  
Frying Temperature ◽  
Simultaneous Quantification ◽  
Frying Process

A simple and reliable HPLC method was developed and validated for the simultaneous quantification of four major constituents in Semen Vaccariae. The chromatographic separation was performed on an Agilent Zorbax SB-C18 column with gradient elution using methanol and water. The calibration curves showed good linearity of R2 > 0.9999 with LOQs (S/N = 10) of 0.20–1.16 μg/mL. The precision was evaluated by intra- and inter-day assays and R.S.D. values were less than 2.09%. The recovery rates were between 97.0% and 105.0%. The developed method was applied to the quantitative analysis of Semen Vaccariae and its stir-fried products. During the stir-frying process, vaccarin degraded and yielded isovitexin-2″- O-arabinoside. The preferable stir-frying temperature is around 120°C. The developed HPLC method can be applied to the quality control of crude and stir-fried Semen Vaccariae.

Simultaneous determination of multiple active components in Cynomorium songaricum by HPLC method

2014 ◽  
Vol 14 (2) ◽  
pp. 122-124
Author(s):  
HanChun YANG ◽  
Rong WANG ◽  
PengXiao XIA ◽  
WenChao JIA ◽  
ChangYong YANG
Keyword(s):  
Simultaneous Determination ◽  
Hplc Method ◽  
Active Components

Simultaneous quantitation of nine kinds of (d)- and (l)-amino acid enantiomers by HPLC-MS/MS: application to the quality control of amino acid tablets

2015 ◽  
Vol 7 (20) ◽  
pp. 8817-8825 ◽  
Author(s):  
Xiaoyan Li ◽  
Yan Cui ◽  
Yuping Xing ◽  
Chunxiao Lv ◽  
Qing Li ◽  
...  
Keyword(s):  
Quality Control ◽  
Amino Acid ◽  
Simultaneous Determination ◽  
Amino Acid Enantiomers

A HPLC-MS/MS method was developed for the simultaneous determination of nine kinds of (d)- and (l)-amino acid enantiomers and applied to control the quality of amino acid tablets.

Sign in / Sign up

Export Citation Format

Share Document