Marker compounds for the determination of retention factors in EKC

2009 ◽  
Vol 33 (3) ◽  
pp. 394-409 ◽  
Author(s):  
Susanne K. Wiedmer ◽  
Jana Lokajová ◽  
Marja-Liisa Riekkola
Keyword(s):  
Retention Factors ◽  
Marker Compounds

Determination of retention factors of phospholipids in gradient HPLC

2000 ◽  
Vol 51 (1-2) ◽  
pp. 61-64 ◽  
Author(s):  
K. H. Row ◽  
J. W. Lee
Keyword(s):  
Retention Factors ◽  
Gradient Hplc

scholarly journals Simultane ous Determination of Protopseudohypericin, Pseudohypericin, Protohypericin, and Hypericin Without Light Exposure

2005 ◽  
Vol 88 (6) ◽  
pp. 1607-1612 ◽  
Author(s):  
Steven F Baugh
Keyword(s):  
Free Radical ◽  
Light Exposure ◽  
Radical Reactions ◽  
Mathematical Relationship ◽  
Free Radical Reactions ◽  
St John's Wort ◽  
Naturally Occurring ◽  
Analytical Response ◽  
Marker Compounds

Abstract St. John's wort products are commonly standardized to total naphthodianthrones and hyperforin. Determination of these marker compounds is complicated because of the photochemistry of the naphthodianthrones pseudohypericin and hypericin and the instability of hyperforin in solution. Protopseudohypericin and protohypericin have been identified as naturally occurring naphthodianthrones and, when exposed to light, they are converted into pseudohypericin and hypericin, respectively. However, exposure to light and the resulting naphthodianthrone free-radical reactions oxidize hyperforin. A mathematical relationship between the response of the proto compound and the resulting naphthodianthrone can be established by comparing the analytical response of the proto compound in a solution protected from light with the increase in the analytical response of naphthodianthrone in the same solution after exposure to light. By mathematically converting the proto compounds to their respective products, exposure to light can be avoided while still including proto compounds in a single assay. The method presented here details the reporting of all significant naphthodianthrones, including protopseudohypericin and protohypericin, without exposure to light. This approach includes the benefits of improved naphthodianthrone precision and protection of hyperforin from oxidation.

Simultaneous Determination of 11 Marker Compounds in Gumiganghwal-tang by HPLC-DAD and LC-MS

2016 ◽  
Vol 22 (4) ◽  
pp. 238 ◽  
Author(s):  
Jin Bae Weon ◽  
Youn Sik Jung ◽  
Gahee Ryu ◽  
Woo Seung Yang ◽  
Choong Je Ma
Keyword(s):  
Simultaneous Determination ◽  
Marker Compounds

scholarly journals Rapid Screening for FlavoneC-Glycosides in the Leaves of Different Species of Bamboo and Simultaneous Quantitation of Four Marker Compounds by HPLC-UV/DAD

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Jin Wang ◽  
Yong-de Yue ◽  
Hao Jiang ◽  
Feng Tang
Keyword(s):  
Leaf Extract ◽  
Hplc Method ◽  
Rapid Screening ◽  
Qualitative Survey ◽  
Bamboo Leaves ◽  
Multiple Wavelength ◽  
Photodiode Array ◽  
Marker Compounds ◽  
Wavelength Detector

A strategy for analyzing flavoneC-glucosides in the leaves of different species of bamboo was developed. Firstly, the flavoneC-glycosides were extracted from the bamboo leaves (51 species in 17 genera) with methanol and chromatographed on silica gel 60 plates in automatic developing chamber (ADC2), and a qualitative survey using simple derivatization steps with the NP reagent was carried out. The flavoneC-glycosides were found in 40 of 51 species of bamboo examined. Secondly, an HPLC method with photodiode array and multiple wavelength detector was optimized and validated for the simultaneous determination of flavoneC-glycosides, including isoorientin, isovitexin, orientin, and vitexin in the leaves of three species of bamboo and the flavoneC-glycosides were confirmed by LC/MS. The optimized HPLC method proved to be linear in the concentration range tested (0.2–100 μg/mL,r2≥0.9997), precise (RSD≤1.56%), and accurate (88–106%). The concentration ranges of isoorientin, isovitexin, orientin, and vitexin in three bamboo leaves samples were 1.00–2.78, 0–0.31, 0–0.07, and 0.20–0.68 mg/g, respectively. The proposed method was validated to be simple and reliable and can be a tool for quality control of bamboo leaf extract or its commercial products.

scholarly journals Silica gel functionalized with imidazolium group via click chemistry – new stationary phase for ion chromatography

2021 ◽  
Vol 8 (4) ◽  
pp. 20218409
Author(s):  
D. A. Chuprynina ◽  
I. A. Lupanova ◽  
V. V. Konshin ◽  
D. N. Konshina
Keyword(s):  
Ion Exchange ◽  
Stationary Phase ◽  
Silica Gel ◽  
Separation Efficiency ◽  
Click Reaction ◽  
Exchange Chromatography ◽  
Retention Factors ◽  
Exchange Material ◽  
Ph Range

This manuscript describes the preparation of a simple effective ion-exchange material based on silica gel, on the surface of which methylimidazolium bromide is fixed using a click reaction. The resulting material was used as a stationary phase for the separation and determination of Cl–, NO2–, NO3–, I–, and SO42– using ion exchange chromatography. The separation efficiency and retention factors for the selected anions were studied in the pH range 3.5–6.5. The proposed material was used for the determination of Cl–, SO42– in water and can be suggested for successful use in real water samples.

scholarly journals Analysis of Flavonids and Terpenoids in Ethanol Extract of Colocasia esculenta L. (Schoot) Stalk and Leaves

2021 ◽  
Vol 5 (4) ◽  
pp. 359-364
Author(s):  
Erni Rustiani ◽  
Aulia Fitriani ◽  
Sri Wardatun
Keyword(s):  
Developing Countries ◽  
Petroleum Ether ◽  
Central America ◽  
Ethanol Extract ◽  
Colocasia Esculenta ◽  
Marker Compounds

Colocasia esculenta L. (Schoot) is a widely used plant in developing countries in Asia, Africa, and Central America. The parts of the Colocasia plant that can be used are the tubers, stalks, and leaves. There has not been much research on the content of this plant. The study aims to determine the flavonoids and terpenoids in the ethanol extract of Colocasia stalk and leaves. The maceration method with 70% ethanol solvent and drying with vacuum evaporator. Determination of flavonoid levels based on quercetin marker compounds using AlCl3 reagent by UV-Vis spectrophotometry. The terpenoid levels were carried out gravimetrically with petroleum ether as a solvent. The results showed that the ethanol extract of Colocasia stalk and leaf contained flavonoids, respectively 3.18 +0.0581% and 4.33 +0.0285%, while the results of the terpenoid levels for stalks were 7.10 +0.0676% and leaves were 8.39 +0.0023%.

scholarly journals Simultaneous Determination of Four Marker Compounds in Lobelia chinensis Lour. Extract by HPLC-PDA

2021 ◽  
Vol 11 (24) ◽  
pp. 12080
Author(s):  
Beom-Geun Jo ◽  
Young-Hun Park ◽  
Ki Hyun Kim ◽  
Su-Nam Kim ◽  
Min Hye Yang
Keyword(s):  
Formic Acid ◽  
High Performance ◽  
Reverse Phase ◽  
High Performance Liquid Chromatograph ◽  
Liquid Chromatograph ◽  
Snake Bites ◽  
Antiviral Activities ◽  
Photodiode Array ◽  
Marker Compounds

Lobelia chinensis Lour. (L. chinensis) has traditionally been used as a treatment for snake bites, high fever, jaundice, edema, and diarrhea, and modern studies have reported its anti-inflammatory, antioxidant, and antiviral activities. L. chinensis contains various compounds, such as flavonoids and coumarins, and its flavonoid components have been identified in many studies. In this study, a high-performance liquid chromatograph equipped with a photodiode array (PDA) detector and an Aegispak C18-L reverse-phase column (4.6 mm × 250 mm i.d., 5 μm) was used to simultaneously analyze four marker components in L. chinensis for standardization purposes. HPLC-PDA (detection at 340 nm), performed using a 0.1% formic acid-water/0.1% formic acid-acetonitrile gradient, separated the four marker compounds: luteolin-7-O-β-d-glucuronopyranosyl (1→2)-O-β-d-glucuronopyranoside, clerodendrin, chrysoeriol-7-O-diglucuronide, and diosmin. The developed analytical method showed excellent linearity values (r2 > 0.9991), limits of detection (LODs: 0.376–2.152 μg/mL), limits of quantification (LOQs: 1.147–6.521 μg/mL), intra- and inter-day precisions (RSD < 1.96%), and analyte recoveries (96.83–127.07%; RSD < 1.73%); thus, it was found to be suitable for the simultaneous analysis of these four marker compounds in L. chinensis.

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