Method to predict the bandwidth of elution profile under the linear gradient elution in reversed-phase HPLC

2009 ◽  
Vol 32 (2) ◽  
pp. 221-230 ◽  
Author(s):  
Ju Weon Lee ◽  
Kyung Ho Row
Keyword(s):  
Gradient Elution ◽  
Reversed Phase ◽  
Elution Profile ◽  
Reversed Phase Hplc ◽  
Linear Gradient

Gradient elution in reversed-phase HPLC-separation of macromolecules

1983 ◽  
Vol 55 (14) ◽  
pp. 1412A-1430A ◽  
Author(s):  
L. R. Snyder ◽  
M. A. Stadalius ◽  
M. A. Quarry
Keyword(s):  
Gradient Elution ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Hplc Separation

Allantoin as a marker of oxidative stress in human erythrocytes

2008 ◽  
Vol 46 (9) ◽  
Author(s):  
Roman Kand'ár ◽  
Pavla Žáková
Keyword(s):  
Oxidative Stress ◽  
Renal Failure ◽  
Uric Acid ◽  
Chronic Renal Failure ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Control Group ◽  
Uric Acid Concentration ◽  
Reversed Phase Hplc

Abstract: Uric acid is the final product of purine metabolism in humans. It was determined that this compound has important antioxidative properties and it may be oxidized to allantoin by various reactive oxygen species. Therefore, the measurement of allantoin may be useful for the determination of oxidative stress in humans.: We measured allantoin and uric acid in human plasma and erythrocytes obtained from patients with chronic renal failure before hemodialysis (n=30) and blood donors (n=30). We used a method based on selective isolation of allantoin from deproteinized plasma and erythrocyte lysate samples on AG 1-X8 resin and its derivatization to glyoxylate-2, 4-dinitrophenylhydrazone. Separation of glyoxylate-2, 4-dinitrophenylhydrazone from interfering substances was achieved on reversed phase HPLC with gradient elution and UV/VIS detection at 360 nm. Uric acid was determined by reversed phase HPLC with UV/VIS detection at 292 nm.: We found significant differences in allantoin and uric acid concentration between the patients with chronic renal failure and the control group both in plasma (20.5±6.5 μmol/L and 323.9±62.9 μmol/L vs. 2.1±1.1 μmol/L and 270.1±62.3 μmol/L, p<0.05) and erythrocytes [82.8±39.1 nmol/g hemoglobin (Hb) and 110.7±28.8 nmol/g Hb vs. 20.1±6.1 nmol/g Hb and 82.1±23.7 nmol/g Hb, p<0.05].: Significant higher levels of allantoin in both plasma and erythrocytes of patients with chronic renal failure indicate that allantoin may be used as a good marker of oxidative stress.Clin Chem Lab Med 2008;46:1270–4.

Optimization of peptide separations in reversed-phase HPLC: Isocratic versus gradient elution

1987 ◽  
Vol 24 (1) ◽  
pp. 565-572 ◽  
Author(s):  
C. T. Mant ◽  
T. W. Lorne Burke ◽  
R. S. Hodges
Keyword(s):  
Gradient Elution ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Peptide Separations

High-performance liquid chromatography of pyruvic and alpha-ketoglutaric acids and its application to urine samples.

1977 ◽  
Vol 23 (5) ◽  
pp. 802-805 ◽  
Author(s):  
J C Liao ◽  
N E Hoffman ◽  
J J Barboriak ◽  
D A Roth
Keyword(s):  
Aqueous Solution ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Ammonium Acetate ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Diabetic Patients ◽  
Reversed Phase Chromatography ◽  
Linear Gradient ◽  
Normal Individuals

Abstract Pyruvic and alpha-ketoglutaric acids were quantitatively and rapidly converted to quinoxalones by a new method, to enhance their detectability in the ultraviolet. The quinoxalones were separated by reversed-phase chromatography with use of 10 micronm particles packed in a 30-cm (length) column. An aqueous solution of ammonium acetate and methanol was used for linear gradient elution. The urinary excretion of pyruvic and alpha-ketoglutaric acids by normal individuals, diabetic patients, and patients with renal dysfunction was determined.

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