Isolation and characterization of a variant of B16-mouse melanoma resistant to MSH growth inhibition

1979 ◽  
Vol 11 (2) ◽  
pp. 251-258 ◽  
Author(s):  
Richard M. Niles ◽  
Mary P. Logue
Keyword(s):  
Growth Inhibition ◽  
Mouse Melanoma ◽  
Isolation And Characterization ◽  
B16 Mouse Melanoma

Isolation and characterization of glycoconjugates from B16 mouse melanoma tumors

1978 ◽  
Vol 65 (1) ◽  
pp. 73-83 ◽  
Author(s):  
V. Sheik Fareed ◽  
V.P. Bhavanandan ◽  
E.A. Davidson
Keyword(s):  
Mouse Melanoma ◽  
Isolation And Characterization ◽  
B16 Mouse Melanoma

Growth inhibition and induction of phenotypic alterations by l-histidinol in B16 mouse melanoma cells

1989 ◽  
Vol 47 (3) ◽  
pp. 193-197 ◽  
Author(s):  
J. Nordenberg ◽  
L. Wasserman ◽  
H. Gutman ◽  
E. Beery ◽  
A. Novogrodsky
Keyword(s):  
Growth Inhibition ◽  
Melanoma Cells ◽  
Mouse Melanoma ◽  
B16 Mouse Melanoma

scholarly journals Isolation and characterization of mutant CHO cell lines with compartment-specific resistance to brefeldin A.

1994 ◽  
Vol 126 (1) ◽  
pp. 65-75 ◽  
Author(s):  
J P Yan ◽  
M E Colon ◽  
L A Beebe ◽  
P Melançon
Keyword(s):  
Growth Inhibition ◽  
Cell Lines ◽  
Brefeldin A ◽  
Cho Cell ◽  
Isolation And Characterization ◽  
Early Endosomes ◽  
Intracellular Target ◽  
And Function ◽  
Mutant Lines

22 CHOBFY (BFY) cell lines were isolated at a frequency 2-30 x 10(-7) from mutagenized populations on the basis of their ability to grow in the presence of 1 microgram/ml brefeldin A (BFA). Four of the five mutant lines tested are genetically stable and none of the mutant lines characterized degrade this drug. Immunofluorescence studies reveal that whereas early endosomes and the Golgi complex have nearly identical BFA sensitivities in the parent CHO line, the relative sensitivities of these two organelles were dramatically altered in all six mutant lines tested. Four cell lines maintain normal Golgi appearance at a BFA concentration as high as 10 micrograms/ml. Mutant lines show wide variation in the level of resistance to growth inhibition by BFA, but none of the mutant lines characterized grow above 2 micrograms/ml BFA. This specific growth inhibition is observed under conditions where Golgi morphology and function remain unaffected, suggesting that some factor(s) unrelated to Golgi function remains sensitive to BFA in BFY mutant lines. These observations provide strong evidence for the presence of multiple, organelle-specific targets for BFA. Cell-free measurements with membrane extracts establish that resistance to BFA in BFY-1 cells involves a membrane-associated factor distinct from ARFs and coatomers. This collection of mutant lines may prove valuable for the identification of intracellular target(s) for BFA and/or of effectors that interact upstream or downstream with these targets, thereby uncovering the cascade which regulates assembly of organelle-specific coats.

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