Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell‐wall integrity‐related gene expression

2019 ◽  
Vol 99 (15) ◽  
pp. 6689-6695 ◽  
Author(s):  
Lucía da Cruz Cabral ◽  
Alicia Rodríguez ◽  
Josué Delgado ◽  
Andrea Patriarca
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Cell Wall Integrity ◽  
Related Gene

scholarly journals Cell wall integrity signaling regulates cell wall-related gene expression in Chlamydomonas reinhardtii

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Evan Cronmiller ◽  
Deepak Toor ◽  
Nai Chun Shao ◽  
Thamali Kariyawasam ◽  
Ming Hsiu Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Chlamydomonas Reinhardtii ◽  
Cell Wall Integrity ◽  
Related Gene

scholarly journals Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress

2021 ◽  
Vol 22 (11) ◽  
pp. 5957
Author(s):  
Hyun Jin Chun ◽  
Dongwon Baek ◽  
Byung Jun Jin ◽  
Hyun Min Cho ◽  
Mi Suk Park ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Salt Stress ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Related Gene ◽  
Plant Adaptation ◽  
Salt Stress Response ◽  
Network Analyses ◽  
Cell Wall Biogenesis

Although recent studies suggest that the plant cytoskeleton is associated with plant stress responses, such as salt, cold, and drought, the molecular mechanism underlying microtubule function in plant salt stress response remains unclear. We performed a comparative proteomic analysis between control suspension-cultured cells (A0) and salt-adapted cells (A120) established from Arabidopsis root callus to investigate plant adaptation mechanisms to long-term salt stress. We identified 50 differentially expressed proteins (45 up- and 5 down-regulated proteins) in A120 cells compared with A0 cells. Gene ontology enrichment and protein network analyses indicated that differentially expressed proteins in A120 cells were strongly associated with cell structure-associated clusters, including cytoskeleton and cell wall biogenesis. Gene expression analysis revealed that expressions of cytoskeleton-related genes, such as FBA8, TUB3, TUB4, TUB7, TUB9, and ACT7, and a cell wall biogenesis-related gene, CCoAOMT1, were induced in salt-adapted A120 cells. Moreover, the loss-of-function mutant of Arabidopsis TUB9 gene, tub9, showed a hypersensitive phenotype to salt stress. Consistent overexpression of Arabidopsis TUB9 gene in rice transgenic plants enhanced tolerance to salt stress. Our results suggest that microtubules play crucial roles in plant adaptation and tolerance to salt stress. The modulation of microtubule-related gene expression can be an effective strategy for developing salt-tolerant crops.

ABA accelerates blackberry (Rubus spp.) fruit ripening by positively affecting ripening-related gene expression and metabolite profiles

2021 ◽  
pp. 1-16
Author(s):  
Chunhong Zhang ◽  
Yaqiong Wu ◽  
Zhenghao Xiong ◽  
Weilin Li ◽  
Wenlong Wu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Fruit Ripening ◽  
Plant Hormone ◽  
Expression Profiles ◽  
Related Gene ◽  
Commercial Use ◽  
Metabolite Profiles ◽  
Metabolite Accumulation ◽  
Aba Synthesis

BACKGROUND: The softness of blackberry fruits limits their postharvest shelf-life and commercial use, and abscisic acid (ABA) is considered one of the key hormones involved in fruit ripening. OBJECTIVE: This study aimed to explore the underlying physiological and molecular actions of ABA on blackberry fruit ripening and softening. METHODS: Various physiological indices of and plant hormone levels in treated and untreated blackberry fruits were determined simultaneously. The differentially expressed genes (DEGs) were analyzed by RNA-sequencing, and their expression profiles were detected. The ripening mechanism was elucidated by UHPLC-MS using two groups of fruits at 28 d. RESULTS: After 25 d, the ABA concentration and polygalacturonase (PG) and beta-1,4-endoglucanase (EG) activities in ABA-treated fruits were significantly higher than those in untreated fruits. Large differences in the expression profiles were detected at 28 d. The expression of DEGs related to cell wall softening and ABA synthesis was largely triggered after 25 or 28 d. Sixty-nine differentially accumulated metabolites were ultimately annotated as related to fruit ripening. CONCLUSIONS: ABA stimulates blackberry fruit ripening by promoting cell wall enzyme activities, the expression of various ripening-related genes and metabolite accumulation.

Cell wall metabolism and related gene expression inMalus domesticaBorkh. during fruit growth and softening

Fruits ◽  
2015 ◽  
Vol 70 (3) ◽  
pp. 153-161 ◽  
Author(s):  
Xiu-dong Qi ◽  
Jian-mei Wei ◽  
Haishan Li ◽  
Dan Zhao
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Fruit Growth ◽  
Related Gene ◽  
Cell Wall Metabolism

scholarly journals Deregulation of DSE1 Gene Expression Results in Aberrant Budding within the Birth Scar and Cell Wall Integrity Pathway Activation in Saccharomyces cerevisiae

2009 ◽  
Vol 8 (4) ◽  
pp. 586-594 ◽  
Author(s):  
Ivana Frýdlová ◽  
Ivana Malcová ◽  
Pavla Vašicová ◽  
Jiří Hašek
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Daughter Cell ◽  
Fluorescent Protein ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Specific Protein ◽  
Cell Wall Integrity ◽  
Pathway Activation

ABSTRACT Strains of Saccharomyces cerevisiae lacking Isw2, the catalytic subunit of the Isw2 chromatin remodeling complex, show the mating type-independent activation of the cell wall integrity (CWI) signaling pathway. Since the CWI pathway activation usually reflects cell wall defects, we searched for the cell wall-related genes changed in expression. The genes DSE1, CTS1, and CHS1 were upregulated as a result of the absence of Isw2, according to previously published gene expression profiles (I. Frydlova, M. Basler, P. Vasicova, I. Malcova, and J. Hasek, Curr. Genet. 52:87-95, 2007). Western blot analyses of double deletion mutants, however, did not indicate the contribution of the chitin metabolism-related genes CTS1 and CHS1 to the CWI pathway activation. Nevertheless, the deletion of the DSE1 gene encoding a daughter cell-specific protein with unknown function suppressed CWI pathway activation in isw2Δ cells. In addition, the deletion of DSE1 also abolished the budding-within-the-birth-scar phenotype of isw2Δ cells. The plasmid-driven overexpression proved that the deregulation of Dse1 synthesis was also responsible for CWI pathway activation and manifestation of the budding-within-the-birth-scar phenotype in wild-type cells. The overproduced Dse1-green fluorescent protein localized to both sides of the septum and persisted in unbudded cells. Although the exact cellular role of this daughter cell-specific protein has to be elucidated, our data point to the involvement of Dse1 in bud site selection in haploid cells.

scholarly journals Cell wall integrity modulates Arabidopsis thaliana cell cycle gene expression in a cytokinin- and nitrate reductase-dependent manner

Development ◽  
2018 ◽  
Vol 145 (19) ◽  
pp. dev166678 ◽  
Author(s):  
Nora Gigli-Bisceglia ◽  
Timo Engelsdorf ◽  
Miroslav Strnad ◽  
Lauri Vaahtera ◽  
Ghazanfar Abbas Khan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Nitrate Reductase ◽  
Cell Wall Integrity ◽  
Cell Cycle Gene ◽  
Dependent Manner ◽  
Cell Cycle Gene Expression

scholarly journals Gene Expression ofPneumocystis murinaafter Treatment with Anidulafungin Results in Strong Signals for Sexual Reproduction, Cell Wall Integrity, and Cell Cycle Arrest, Indicating a Requirement for Ascus Formation for Proliferation

2018 ◽  
Vol 62 (5) ◽  
Author(s):  
Melanie T. Cushion ◽  
Alan Ashbaugh ◽  
Keeley Hendrix ◽  
Michael J. Linke ◽  
Nikeya Tisdale ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Cell Wall ◽  
Life Cycle ◽  
Cell Cycle Arrest ◽  
Expression Profiles ◽  
Life Cycle Stage ◽  
Cell Wall Integrity ◽  
Content Type ◽  
Cycle Arrest

ABSTRACTThe echinocandins are a class of antifungal agents that target β-1,3-d-glucan (BG) biosynthesis. In the ascigerousPneumocystisspecies, treatment with these drugs depletes the ascus life cycle stage, which contains BG, but large numbers of forms which do not express BG remain in the infected lungs. In the present study, the gene expression profiles ofPneumocystis murinawere compared between infected, untreated mice and mice treated with anidulafungin for 2 weeks to understand the metabolism of the persisting forms. Almost 80 genes were significantly up- or downregulated. Like other fungi exposed to echinocandins, genes associated with sexual replication, cell wall integrity, cell cycle arrest, and stress comprised the strongest upregulated signals inP. murinafrom the treated mice. The upregulation of theP. murinaβ-1,3-d-glucan endohydrolase and endo-1,3-glucanase was notable and may explain the disappearance of the existing asci in the lungs of treated mice since both enzymes can degrade BG. The biochemical measurement of BG in the lungs of treated mice and fluorescence microscopy with an anti-BG antibody supported the loss of BG. Downregulated signals included genes involved in cell replication, genome stability, and ribosomal biogenesis and function and thePneumocystis-specific genes encoding the major surface glycoproteins (Msg). These studies suggest thatP. murinaattempted to undergo sexual replication in response to a stressed environment and was halted in any type of proliferative cycle, likely due to a lack of BG. Asci appear to be a required part of the life cycle stage ofPneumocystis, and BG may be needed to facilitate progression through the life cycle via sexual replication.

scholarly journals Penetration Peg Formation and Invasive Hyphae Development Require Stage-Specific Activation of MoGTI1 in Magnaporthe oryzae

2016 ◽  
Vol 29 (1) ◽  
pp. 36-45 ◽  
Author(s):  
Yang Li ◽  
Guanghui Wang ◽  
Jin-Rong Xu ◽  
Cong Jiang
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Protein Kinase ◽  
Magnaporthe Oryzae ◽  
Phosphorylation Site ◽  
Cell Wall Integrity ◽  
Invasive Growth ◽  
Appressorium Formation ◽  
Plant Infection ◽  
Cdk Phosphorylation

The hemibiotrophic pathogen Magnaporthe oryzae causes one of the most destructive diseases in cultivated rice. Complex infection-related morphogenesis and production of various effectors are known to be important for successful colonization and disease development. In this study, we characterized the activation of the MoGTI1 transcription factor and its role in infection-related morphogenesis and effector gene expression. The Mogti1 mutant was nonpathogenic, although it was normal in appressorium formation and turgor generation. Close examination showed that Mogti1 was defective in penetration and growth of normal invasive hyphae. Deletion of MoGTI1 affected the expression of the majority of effector genes. The expression of MoGti1 appeared to be controlled by the Mps1 but not Pmk1 mitogen-activated protein kinase (MAPK), and the mps1 and Mogti1 mutants had similar phenotypes in plant infection and cell wall integrity defects. However, lack of MAPK phosphorylation sites and dispensability of the putative MAPK docking site suggested that MoGti1 is not a direct target of Mps1. Site-specific mutagenesis analyses showed that the putative protein kinase A phosphorylation site was not essential for localization of MoGti1 to the nucleus but important for its normal function. Although the cyclin-dependent kinase (CDK) phosphorylation site of MoGti1 is dispensable during vegetative growth and appressorium formation, the S77A mutation affected penetration and invasive growth. Localization of MoGti1S77A-green fluorescent protein to the nucleus in late stages of appressorium formation and during invasive growth was not observed, suggesting a stage-specific CDK phosphorylation of MoGti1. Overall, our data indicate that Mps1 may indirectly regulate the expression of MoGti1 in maintaining cell wall integrity, conidiation, and plant infection. MoGti1 is likely a stage-specific target of CDK and plays a crucial role in effector gene expression and morphogenesis related to the development of penetration pegs and invasive hyphae.

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