scholarly journals Cell wall integrity modulates Arabidopsis thaliana cell cycle gene expression in a cytokinin- and nitrate reductase-dependent manner

Development ◽  
2018 ◽  
Vol 145 (19) ◽  
pp. dev166678 ◽  
Author(s):  
Nora Gigli-Bisceglia ◽  
Timo Engelsdorf ◽  
Miroslav Strnad ◽  
Lauri Vaahtera ◽  
Ghazanfar Abbas Khan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Nitrate Reductase ◽  
Cell Wall Integrity ◽  
Cell Cycle Gene ◽  
Dependent Manner ◽  
Cell Cycle Gene Expression

scholarly journals Cellulose biosynthesis inhibition reduces cell cycle activity in a nitrate reductase- and cytokinin-dependent manner

2018 ◽  
Author(s):  
Nora Gigli-Bisceglia ◽  
Timo Engelsdorf ◽  
Miroslav Strnad ◽  
Lauri Vaahtera ◽  
Amel Jamoune ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Cell Wall ◽  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Cell Wall Integrity ◽  
Cell Cycle Gene ◽  
List Type ◽  
Cellulose Biosynthesis ◽  
Cell Cycle Gene Expression

SummaryDuring growth, development and defense, cell wall integrity needs to be coordinated with cell cycle activity. In Saccharomyces cerevisiae, coordination is mediated by the cell wall integrity maintenance mechanism. In plants, little is known how coordination is achieved.Here we investigated coordination between plant cell wall and cell cycle activity in Arabidopsis thaliana seedlings by studying the impact of cell wall damage (CWD, caused by cellulose biosynthesis inhibition) on cell cycle gene expression, growth, phytohormone (jasmonic acid, salicylic acid, cytokinins) and lignin accumulation.We found root growth and cell cycle gene expression are reduced by CWD in an osmo-sensitive manner. trans-zeatin application suppressed the CWD effect on gene expression. Quantification of cytokinins revealed CWD-induced, osmo-sensitive changes in several cytokinins. Expression of CYTOKININ OXIDASE2/DEHYDROGENASE (CKX2) and CKX3, encoding cytokinin-degrading enzymes, was elevated in CWD-exposed seedlings. Genetic studies implicated NITRATE REDUCTASE1/2 (NIA1/2) in the response to CWD. In nia1/2 seedlings CWD induced neither expression of CKX2/3 and cell cycle genes nor accumulation of jasmonic acid, salicylic acid and lignin.This suggests that CWD causes increased CKX2/3 expression through a NIA1/2-mediated process. Increased CKX expression seems to cause changes in cytokinin levels, leading to reduced cell cycle gene expression.

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression

scholarly journals Altered Cell Cycle Gene Expression and Apoptosis in Post-Implantation Dog Parthenotes

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e41256 ◽  
Author(s):  
Jung Eun Park ◽  
Min Jung Kim ◽  
Seung Kwon Ha ◽  
So Gun Hong ◽  
Hyun Ju Oh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Cell Cycle Gene ◽  
Altered Cell ◽  
Post Implantation ◽  
Cell Cycle Gene Expression

scholarly journals Global Analysis of Host Cell Gene Expression Late during Cytomegalovirus Infection Reveals Extensive Dysregulation of Cell Cycle Gene Expression and Induction of Pseudomitosis Independent of US28 Function

2004 ◽  
Vol 78 (21) ◽  
pp. 11988-12011 ◽  
Author(s):  
Laura Hertel ◽  
Edward S. Mocarski
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Dna Replication ◽  
Host Cell ◽  
Small Gtpases ◽  
Global Changes ◽  
Cell Cycle Gene ◽  
Gene Products ◽  
Cell Gene ◽  
Cell Cycle Gene Expression

ABSTRACT Replication of human cytomegalovirus (CMV) depends on host cell gene products working in conjunction with viral functions and leads to a dramatic dysregulation of cell cycle gene expression. Comprehensive transcriptional profiling was used to identify pathways most dramatically modulated by CMV at late times during infection and to determine the extent to which expression of the viral chemokine receptor US28 contributed to modulating cellular gene expression. Cells infected with the AD169 strain of virus or a fully replication competent US28-deficient derivative (RV101) were profiled throughout the late phase of infection (50, 72, and 98 h postinfection). Although sensitive statistical analysis showed striking global changes in transcript levels in infected cells compared to uninfected cells, the expression of US28 did not contribute to these alterations. CMV infection resulted in lower levels of transcripts encoding cytoskeletal, extracellular matrix, and adhesion proteins, together with small GTPases and apoptosis regulators, and in higher levels of transcripts encoding cell cycle, DNA replication, energy production, and inflammation-related gene products. Surprisingly, a large number of cellular transcripts encoding mitosis-related proteins were upmodulated at late times in infection, and these were associated with the formation of abnormal mitotic spindles and the appearance of pseudomitotic cells. These data extend our understanding of how broadly CMV alters the regulation of host cell cycle gene products and highlight the establishment of a mitosis-like environment in the absence of cellular DNA replication as important for viral replication and maturation.

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