Tracking the behavior of Maillard browning in lysine/arginine-sugar model systems under high hydrostatic pressure

2017 ◽  
Vol 97 (15) ◽  
pp. 5168-5175 ◽  
Author(s):  
Xiao-juan Ma ◽  
Jin-yan Gao ◽  
Ping Tong ◽  
Xin Li ◽  
Hong-bing Chen
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Model Systems ◽  
Maillard Browning

Effect of High Hydrostatic Pressure Processing on Lycopene Isomers

2010 ◽  
Vol 6 (5) ◽  
Author(s):  
Supriya Varma ◽  
Mukund V Karwe ◽  
Tung-Ching Lee
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Food Systems ◽  
Processing Technology ◽  
Model Systems ◽  
Isomeric Form ◽  
Trans Content ◽  
Preparative Scale ◽  
Isomer Content ◽  
Cis Isomer

Lycopene is known for its preventive mechanism of action against various types of cancers and chronic diseases. It is an acyclic C40 non-polar carotenoid that is found in tomatoes in various geometrical isomeric forms. In nature, the predominant form is the all-trans isomeric form (80-97%) in tomato and tomato related products. However research indicates that >50% of the lycopene in human body is found in the cis isomeric form, thereby leading to the hypothesis that the cis form is the more bioavailable /bioactive form in the human body.Today’s consumers seek processed products that are minimally processed and have retained their overall quality. This has lead scientists to explore non–thermal processing technologies like High Hydrostatic Pressure (HHP) processing technology. Our hypothesis that energy input can cause isomerization lead us to study the increase in cis isomer content on using HHP processing technology. Semi preparative scale HPLC method was used to purify the isomers. Lycopene isomers in tributyrin (model systems) and tomato homogenate (real food systems) were used. The HHP equipment was manufactured by Elmhurst Research, Inc. It is a 10L tilt vessel assembly with horizontal loading and vertical operation. The pressure treatment varied from 320Mpa-620Mpa for 3 minutes at ambient conditions. Treatment of both the systems showed an increase in the cis isomer content compared to the control. The all-trans content reduced by ~70% in model systems and the cis content increased by ~44% in model systems. The all-trans content increased by ~50% in food systems and the cis content also increased by ~35% in food systems. The results indicate that pressure causes conformational change from the all-trans to cis isomer form; thereby indicating that high-pressure application can induce cis isomerization.

scholarly journals High-pressure-mediated dissociation of immune complexes demonstrated in model systems

1998 ◽  
Vol 44 (2) ◽  
pp. 299-303 ◽  
Author(s):  
Charles Y Cheung ◽  
David J Green ◽  
Gerald J Litt ◽  
James A Laugharn
Keyword(s):  
High Pressure ◽  
Hydrostatic Pressure ◽  
Prostate Specific Antigen ◽  
Immune Complexes ◽  
High Hydrostatic Pressure ◽  
Room Temperature ◽  
Specific Antigen ◽  
Model Systems ◽  
Mucin Glycoprotein ◽  
Protein Dissociation

Abstract The use of pressure to disrupt immune complexes was demonstrated in two model systems: prostate-specific antigen (PSA) and anti-PSA antibody; and epiglycanin, a mucin glycoprotein, and an antibody specific to that protein. Dissociation of the anti-PSA antibody from the immobilized PSA antigen was observed when pressures of 415 MPa and 550 MPa (1 MPa ∼144 psi) were applied at room temperature (∼21 °C). Application of pressures ranging from 140 MPa to 550 MPa resulted in dissociation of antibody from epiglycanin. In both cases, the rebinding of dissociated antibody to immobilized antigen indicated that the effect of high pressure on the binding of the immune complexes was reversible. These findings suggest that application of high hydrostatic pressure has the potential to be used to significantly improve the sensitivity and specificity of clinical assays.

Oscillatory High Hydrostatic Pressure Inactivation of Zygosaccharomyces bailii

1998 ◽  
Vol 61 (9) ◽  
pp. 1213-1215 ◽  
Author(s):  
ENRIQUE PALOU ◽  
AURELIO LÓPEZ-MALO ◽  
GUSTAVO V. BARBOSA-CÁNOVAS ◽  
JORGE WELTI-CHANES ◽  
BARRY G. SWANSON
Keyword(s):  
Hydrostatic Pressure ◽  
Water Activity ◽  
High Hydrostatic Pressure ◽  
Model Systems ◽  
Laboratory Model ◽  
Initial Inoculum ◽  
Zygosaccharomyces Bailii ◽  
Number Of Cycles ◽  
Oscillatory Pressure

Zygosaccharomyces bailii inactivation was evaluated in oscillatory high hydrostatic pressure (HHP) treatments at sublethal pressures (207, 241, or 276 MPa) and compared with continuous HHP treatments in laboratory model systems with a water activity (aw) of 0.98 and pH 3.5. The yeast was inoculated into laboratory model systems and subjected to HHP in sterile bags. Two HHP treatments were conducted: continuous (holding times of 5, 10, 15, 20, 30, 60, or 90 min) and oscillatory (two, three, or four cycles with holding times of 5 min and two cycles with holding times of 10 min). Oscillatory pressure treatments increased the effectiveness of HHP processing. For equal holding times, Z. bailii counts decreased as the number of cycles increased. Holding times of 20 min in HHP oscillatory treatments at 276 MPa assured inactivation (<10 CFU/ml) of Z. bailii initial inoculum. Oscillatory pressurization could be useful to decrease Z. bailii inactivation time.

Survival of mouse blastocysts after low-temperature preservation under high pressure

2004 ◽  
Vol 52 (4) ◽  
pp. 479-487 ◽  
Author(s):  
Cs. Pribenszky ◽  
M. Molnár ◽  
S. Cseh ◽  
L. Solti
Keyword(s):  
Phase Transition ◽  
Hydrostatic Pressure ◽  
Low Temperature ◽  
High Hydrostatic Pressure ◽  
Room Temperature ◽  
Freezing Point ◽  
Significant Loss ◽  
Embryo Cryopreservation ◽  
Subzero Temperatures ◽  
Survival Capacity

Cryoinjuries are almost inevitable during the freezing of embryos. The present study examines the possibility of using high hydrostatic pressure to reduce substantially the freezing point of the embryo-holding solution, in order to preserve embryos at subzero temperatures, thus avoiding all the disadvantages of freezing. The pressure of 210 MPa lowers the phase transition temperature of water to -21°C. According to the results of this study, embryos can survive in high hydrostatic pressure environment at room temperature; the time embryos spend under pressure without significant loss in their survival could be lengthened by gradual decompression. Pressurisation at 0°C significantly reduced the survival capacity of the embryos; gradual decompression had no beneficial effect on survival at that stage. Based on the findings, the use of the phenomena is not applicable in this form, since pressure and low temperature together proved to be lethal to the embryos in these experiments. The application of hydrostatic pressure in embryo cryopreservation requires more detailed research, although the experience gained in this study can be applied usefully in different circumstances.

Effect of High Hydrostatic Pressure on Endothelial Lipase Expression*

2010 ◽  
Vol 37 (6) ◽  
pp. 641-645 ◽  
Author(s):  
Can-Xin XU ◽  
Chun WANG ◽  
Bing-Yang ZHU ◽  
Zhi-Ping GAO ◽  
Di-Xian LUO ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Endothelial Lipase ◽  
Lipase Expression
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