Cloning of genes related to aliphatic glucosinolate metabolism and the mechanism of sulforaphane accumulation in broccoli sprouts under jasmonic acid treatment

2016 ◽  
Vol 96 (13) ◽  
pp. 4329-4336 ◽  
Author(s):  
Liping Guo ◽  
Runqiang Yang ◽  
Zhenxin Gu
Keyword(s):  
Jasmonic Acid ◽  
Acid Treatment ◽  
Broccoli Sprouts ◽  
Glucosinolate Metabolism ◽  
Aliphatic Glucosinolate

scholarly journals Effect of jasmonic acid on glucosinolate metabolism in different organs of broccoli sprouts

2019 ◽  
pp. 81
Author(s):  
Yinglian Zhu, Fengwu Wang, Liping Guo
Keyword(s):  
Jasmonic Acid ◽  
Cancer Prevention ◽  
Broccoli Sprouts ◽  
Health Promoting ◽  
Glucosinolate Metabolism ◽  
Myrosinase Activity

Edible sprouts, especially Brassica sprouts, contain high levels of health-promoting compounds. Exogenous elicitors have been used as strategies to improve the nutraceutical quality of Brassica sprouts. In this study, effects of jasmonic acid (JA) treatment on growth, the levels of glucosinolates and isothiocyanates, as well as myrosinase activity in different organs of broccoli sprouts were investigated. JA treatment markedly increased the contents of glucosinolates (GSLs), especially glucoraphanin, glucobrassicin and neoglucobrassicin in broccoli sprouts. However, gluconapin was not affected even decreased by JA treatment. Cotyledon, hypocotyl and root obtained the different results in induction of GSLs. Among these, neoglucobrassicin obtained the highest enhancement in three organs. Myrosinase activity in cotyledon of broccoli increased after JA treatment, while decreased in hypocotyl. Three concentrations of JA all significantly increased sulforaphane and isothiocyanates formation in cotyledon, hypocotyl and root of broccoli sprouts. Application of 100 μM JA led to the highest myrosinase activity, the least gluconapin and the most sulforaphane and isothiocyanates in cotyledon, as well as the most isothiocyanates in root. These results indicated that JA treatment could be an effective way to improve the cancer-prevention benefits of broccoli sprouts via enhancing sulforaphane and total isothiocyanates.

scholarly journals Postharvest jasmonic acid treatment of sugarbeet roots reduces rot due to Botrytis cinerea, Penicillium claviforme, and Phoma betae

2012 ◽  
Vol 65 ◽  
pp. 1-4 ◽  
Author(s):  
Karen Klotz Fugate ◽  
Jocleita Peruzzo Ferrareze ◽  
Melvin D. Bolton ◽  
Edward L. Deckard ◽  
Larry G. Campbell
Keyword(s):  
Jasmonic Acid ◽  
Botrytis Cinerea ◽  
Acid Treatment ◽  
Penicillium Claviforme ◽  
Phoma Betae

scholarly journals Jasmonic acid treatment to part of the root system is consistent with simulated leaf herbivory, diverting recently assimilated carbon towards untreated roots within an hour

2008 ◽  
Vol 31 (9) ◽  
pp. 1229-1236 ◽  
Author(s):  
GUNNAR JAKOB HENKES ◽  
MICHAEL R. THORPE ◽  
PETER E. H. MINCHIN ◽  
ULRICH SCHURR ◽  
URSULA S. R. RÖSE
Keyword(s):  
Jasmonic Acid ◽  
Root System ◽  
Acid Treatment ◽  
Leaf Herbivory

scholarly journals A comparison of inducible, ontogenetic, and interspecific sources of variation in the foliar metabolome in tropical trees

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7536
Author(s):  
Brian E. Sedio ◽  
Armando Durant Archibold ◽  
Juan Camilo Rojas Echeverri ◽  
Chloé Debyser ◽  
Cristopher A. Boya P ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Acid Treatment ◽  
Tropical Trees ◽  
Tropical Tree ◽  
Molecular Networks ◽  
Ontogenetic Stage ◽  
Chemical Variation ◽  
Leaf Ontogeny ◽  
Secondary Chemistry ◽  
Mature Leaves

Plant interactions with other organisms are mediated by chemistry, yet chemistry varies among conspecific and within individual plants. The foliar metabolome—the suite of small-molecule metabolites found in the leaf—changes during leaf ontogeny and is influenced by the signaling molecule jasmonic acid. Species differences in secondary metabolites are thought to play an important ecological role by limiting the host ranges of herbivores and pathogens, and hence facilitating competitive coexistence among plant species in species-rich plant communities such as tropical forests. Yet it remains unclear how inducible and ontogenetic variation compare with interspecific variation, particularly in tropical trees. Here, we take advantage of novel methods to assemble mass spectra of all compounds in leaf extracts into molecular networks that quantify their chemical structural similarity in order to compare inducible and ontogenetic chemical variation to among-species variation in species-rich tropical tree genera. We ask (i) whether young and mature leaves differ chemically, (ii) whether jasmonic acid-inducible chemical variation differs between young and mature leaves, and (iii) whether interspecific exceeds intraspecific chemical variation for four species from four hyperdiverse tropical tree genera. We observed significant effects of the jasmonic acid treatment for three of eight combinations of species and ontogenetic stage evaluated. Three of the four species also exhibited large metabolomic differences with leaf ontogenetic stage. The profound effect of leaf ontogenetic stage on the foliar metabolome suggests a qualitative turnover in secondary chemistry with leaf ontogeny. We also quantified foliar metabolomes for 45 congeners of the four focal species. Chemical similarity was much greater within than between species for all four genera, even when within-species comparisons included leaves that differed in age and jasmonic acid treatment. Despite ontogenetic and inducible variation within species, chemical differences among congeneric species may be sufficient to partition niche space with respect to chemical defense.

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