Comparison of methods to determine the endogenous amino acid flow at the terminal ileum of the growing rat

1995 ◽  
Vol 67 (3) ◽  
pp. 359-366 ◽  
Author(s):  
Armstrong Donkoh ◽  
Paul J Moughan ◽  
Patrick C H Morel
Keyword(s):  
Amino Acid ◽  
Terminal Ileum ◽  
Comparison Of Methods ◽  
Growing Rat ◽  
Endogenous Amino Acid

Determination of endogenous amino acid flow at the terminal ileum of the rat

1988 ◽  
Vol 44 (3) ◽  
pp. 227-235 ◽  
Author(s):  
Grant A Skilton ◽  
Paul J Moughan ◽  
William C Smith
Keyword(s):  
Amino Acid ◽  
Terminal Ileum ◽  
Endogenous Amino Acid

Endogenous amino acid flows at the terminal ileum of broilers, layers and adult roosters

2004 ◽  
Vol 79 (2) ◽  
pp. 265-271 ◽  
Author(s):  
V. Ravindran ◽  
W. H. Hendriks
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Terminal Ileum ◽  
Crude Protein ◽  
Amino Acid Profile ◽  
The Other ◽  
Endogenous Protein ◽  
Abundant Amino Acid ◽  
Endogenous Amino Acid

AbstractEndogenous flows of nitrogen and amino acids at the terminal ileum of broilers (6 weeks old), layers (70 weeks old) and adult roosters (70 weeks old) were determined using the peptide alimentation method. The ileal endogenous output of nitrogen and total amino acids in broilers, layers and roosters, expressed as mg/kg dry matter intake, were similar (F > 0-05). Endogenous flows were similar (F > 0-05) for nine of the 17 amino acids analysed, but the flows of serine, glutamic acid, proline, alanine, isoleucine, tyrosine, arginine and methionine differed (P < 0-05) among the classes of chickens. The amino acid profile of endogenous protein, expressed asg/100 g crude protein, did not differ (F > 0-05) between the three classes of chickens, except for serine, glutamic acid, proline and isoleucine. The concentrations of proline were higher (F < 0-05) in broilers, compared with the other two groups. The concentrations of glutamic acid in layers were lower (F < 0-05) than the other two groups. The concentrations of serine and isoleucine were higher (F < 0-05) in roosters than the other two groups. In all three groups, the most abundant amino acid in the ileal endogenous protein was glutamic acid, followed by aspartic acid, proline, serine, glycine and threonine. The present study provides estimates for endogenous amino acid flow at the terminal ileum in broilers, layers and adult roosters under normal physiological conditions.

Endogenous Amino Acid Release from Cultured Cerebellar Neuronal Cells: Effect of Tetanus Toxin on Glutamate Release

1989 ◽  
Vol 52 (4) ◽  
pp. 1229-1239 ◽  
Author(s):  
Bernard J. Vliet ◽  
Michèle Sebben ◽  
Aline Dumuis ◽  
Jacqueline Gabrion ◽  
Joël Bockaert ◽  
...  
Keyword(s):  
Amino Acid ◽  
Tetanus Toxin ◽  
Glutamate Release ◽  
Neuronal Cells ◽  
Amino Acid Release ◽  
Acid Release ◽  
Endogenous Amino Acid

Comparison of methods for the detemination of peptides nitrogen in grass silage

1998 ◽  
Vol 1998 ◽  
pp. 152-152
Author(s):  
V. L. Nsereko ◽  
J. A. Rooke
Keyword(s):  
Amino Acid ◽  
Formic Acid ◽  
Amino Acid Analysis ◽  
Initial Study ◽  
Aqueous Extracts ◽  
Comparison Of Methods ◽  
Peptide Analysis ◽  
Trichloracetic Acid ◽  
Grass Silage ◽  
Additive Treatment

There are few reports on the occurrence and amounts of peptide-N in silage. Previous studies (Nsereko and Rooke, 1993, 1996) have quantified the amounts of peptide-N in grass silage and how peptide-N can be modified by additive treatment. However, peptide N concentrations determined using fluorescamine were observed to be considerably higher than those measured by amino acid analysis (Nsereko, 1996). It was the objective of this study to compare methods for the analysis of peptide-N.Triplicate silages treated with no additive, formic acid (5.4 g/kg) or formic acid (2.7 g/kg) and formaldehyde (3.75 g/kg) (Nsereko and Rooke, 1993) were used in an initial study to compare three methods of peptide analysis. Aqueous extracts were prepared from the silages which were deproteinised with trichloracetic acid.

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