Protein extractability and thermal gel formability of myofibrils isolated from skeletal and cardiac muscles at different post-mortem periods

1992 ◽  
Vol 58 (3) ◽  
pp. 385-393 ◽  
Author(s):  
Kunihiko Samejima ◽  
Nam H Lee ◽  
Makoto Ishioroshi ◽  
Ali Asghar
Keyword(s):  
Post Mortem ◽  
Cardiac Muscles ◽  
Protein Extractability

scholarly journals Caspase 9 and Caspase 3 Immunohistochemical Pattern in Skeletal and Cardiac Muscles at Different Times after Death: An Experimental Study on PMI Estimation

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1062
Author(s):  
Cristina Mondello ◽  
Chiara Stassi ◽  
Letteria Minutoli ◽  
Gennaro Baldino ◽  
Angela Alibrandi ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Potential Role ◽  
Interval Estimation ◽  
Immunohistochemical Analysis ◽  
Post Mortem ◽  
Caspase 9 ◽  
Post Mortem Interval ◽  
Cardiac Muscles ◽  
Preliminary Activation ◽  
Pmi Estimation

(1) Background: The estimation of the post mortem interval (PMI) is a challenge for forensic pathologists because data emerging from methods commonly applied are not always conclusive, since several conditions exist that may affect the reliability of these parameters. Thus, new approaches have been proposed to overcome such a limit. In recent years, several studies have been performed on proteins analyzing their expression/degradation patterns in relation to the progressing of the post mortem interval. (2) Methods: The immunoreactivity patterns of two apoptosis mediators—Caspase 9 and Caspase 3—have been tested in order to evaluate their potential role as markers of the post mortem interval. The immunohistochemical analysis was performed on samples of skeletal and cardiac muscles obtained from rats at 0, 4, 8, 12, 24 and 72 h after death. (3) Results: The observed immunoreactivity patterns of both Caspase 9 and Caspase 3 showed a significant correlation with increasing post mortem interval either in skeletal or cardiac muscles, while the comparison of the immunoreactivity patterns of the two apoptotic mediators within each tissue appeared consistent with a preliminary activation of the “initiator” Caspase 9, which, in turn, subsequently activates the “executioner” Caspase 3. (4) Conclusion: The different expressions and decrease immunohistochemically observed on both caspases with progressing PMI support the usefulness of the combined analysis for post mortem interval estimation.

Ultrastructural investigation of an adenoma of the pituitary post mortem

1987 ◽  
Vol 45 ◽  
pp. 622-623
Author(s):  
Shirley Siew ◽  
W. C. deMendonca
Keyword(s):  
Deleterious Effect ◽  
Anterior Lobe ◽  
Definitive Diagnosis ◽  
Pars Intermedia ◽  
Post Mortem ◽  
Close Apposition ◽  
Autopsy Material ◽  
Progressive Loss ◽  
Transmission Electron ◽  
Means Of Transmission

The deleterious effect of post mortem degeneration results in a progressive loss of ultrastructural detail. This had led to reluctance (if not refusal) to examine autopsy material by means of transmission electron microscopy. Nevertheless, Johannesen has drawn attention to the fact that a sufficient amount of significant features may be preserved in order to enable the establishment of a definitive diagnosis, even on “graveyard” tissue.Routine histopathology of the autopsy organs of a woman of 78 showed the presence of a well circumscribed adenoma in the anterior lobe of the pituitary. The lesion came into close apposition to the pars intermedia. Its architecture was more compact and less vascular than that of the anterior lobe. However, there was some grouping of the cells in relation to blood vessels. The cells tended to be smaller, with a higher nucleocytoplasmic ratio. The cytoplasm showed a paucity of granules. In some of the cells, it was eosinophilic.

Two structural states of the Z-band in cardiac and skeletal muscle

1989 ◽  
Vol 47 ◽  
pp. 1022-1023
Author(s):  
J.P. Schroeter ◽  
M.A. Goldstein ◽  
J.P. Bretaudiere ◽  
L.H. Michael ◽  
R.L. Sass
Keyword(s):  
Skeletal Muscle ◽  
Cross Section ◽  
Real Space ◽  
Contractile State ◽  
Contour Maps ◽  
False Color ◽  
Grey Scale ◽  
Fourier Filtering ◽  
Cardiac Muscles ◽  
Enhancement Algorithm

We have recently established the existence of two structural states of the Z band lattice in cross section in cardiac as well as in skeletal muscle. The two structural states are related to the contractile state of the muscle. In skeletal muscle at rest, the Z band is in the small square (ss) lattice form, but tetanized muscle exhibits the basket weave (bw) form. In contrast, unstimu- lated cardiac muscle exhibits the bw form, but cardiac muscles exposed to EGTA show the ss form.We have used two-dimensional computer enhancement techniques on digitized electron micrographs to compare each lattice form as it appears in both cardiac and skeletal muscle. Both real space averaging and fourier filtering methods were used. Enhanced images were displayed as grey-scale projections, as contour maps, and in false color.There is only a slight difference between the lattices produced by the two different enhancement techniques. Thus the information presented in these images is not likely to be an artifact of the enhancement algorithm.

Post-mortem Peyer’s patches: Their potential application in forensic medicine

2009 ◽  
Vol 00 (00) ◽  
pp. 090513010017019-7
Author(s):  
Biagio Solarino ◽  
Giancarlo Di Vella ◽  
Thea Magrone ◽  
Felicita Jirillo ◽  
Angela Tafaro ◽  
...  
Keyword(s):  
Forensic Medicine ◽  
Potential Application ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Post Mortem

Aneurysms in the history of medicine

VASA ◽  
2002 ◽  
Vol 31 (4) ◽  
pp. 281-286 ◽  
Author(s):  
Bollinger ◽  
Rüttimann
Keyword(s):  
History Of Medicine ◽  
Albert Einstein ◽  
Thomas Mann ◽  
Post Mortem ◽  
John Hunter ◽  
History Of

Die Geschichte des sackförmigen oder fusiformen Aneurysmas reicht in die Zeit der alten Ägypter, Byzantiner und Griechen zurück. Vesal 1557 und Harvey 1628 führten den Begriff in die moderne Medizin ein, indem sie bei je einem Patienten einen pulsierenden Tumor intra vitam feststellten und post mortem verifizierten. Weitere Eckpfeiler bildeten die Monographien von Lancisi und Scarpa im 18. bzw. beginnenden 19. Jahrhundert. Die erste wirksame Therapie bestand in der Kompression des Aneurysmasacks von außen, die zweite in der Arterienligatur, der John Hunter 1785 zum Durchbruch verhalf. Endoaneurysmoraphie (Matas) und Umhüllung mit Folien wurden breit angewendet, bevor Ultraschalldiagnostik und Bypass-Chirurgie Routineverfahren wurden und die Prognose dramatisch verbesserten. Die diagnostischen und therapeutischen Probleme in der Mitte des 20. Jahrhunderts werden anhand von zwei prominenten Patienten dargestellt, Albert Einstein und Thomas Mann, die beide im Jahr 1955 an einer Aneurysmaruptur verstarben.

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