The analysis of polyunsaturated fatty acids in meat by capillary gas-liquid chromatography

1982 ◽  
Vol 33 (8) ◽  
pp. 771-776 ◽  
Author(s):  
Andrew J. Sinclair ◽  
William J. Slattery ◽  
Kerin O'Dea
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Polyunsaturated Fatty Acids ◽  
Gas Liquid Chromatography

Cod Flesh: Component Fatty Acids as Determined by Gas–Liquid Chromatography

1964 ◽  
Vol 21 (2) ◽  
pp. 367-371 ◽  
Author(s):  
R. G. Ackman ◽  
R. D. Burgher
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Polyunsaturated Fatty Acids ◽  
Gas Liquid Chromatography ◽  
Very High

The fatty acids of cod flesh, chiefly derived from the phospholipids, are characterized by a very high proportion of polyunsaturated fatty acids and a very low proportion of monoethylenic fatty acids. There appears to be a correlation in the ratios of certain polyunsaturated fatty acids in the liver fatty acids and in the flesh fatty acids.

Determination of Free and Esterified Fatty Acids in Hydrocoles of Different Content of Polyunsaturated Fatty Acids by Gas–liquid Chromatography

2020 ◽  
Vol 75 (10) ◽  
pp. 1310-1321
Author(s):  
A. A. Nikonova ◽  
S. M. Shishlyannikov ◽  
T. A. Shishlyannikova ◽  
T. N. Avezova ◽  
T. A. Babenko ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Polyunsaturated Fatty Acids ◽  
Gas Liquid Chromatography ◽  
Esterified Fatty Acids ◽  
Different Content

scholarly journals INFLUENCE OF COMPOSITION OF POLYUNSATURATED FATTY ACIDS ON MICROVISCOSITY OF THE MEMBRANE OF ERYTHROCYTES OF THE NAVEL OF THE BLOOD AT HERPES INFECTION CONTAMINATIONS

2013 ◽  
Vol 12 (3) ◽  
pp. 37-40
Author(s):  
N. A. Isutina
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Polyunsaturated Fatty Acids ◽  
Data Exchange ◽  
Gas Liquid Chromatography ◽  
Herpes Infection ◽  
Arachidonic Acids

The method of gas-liquid chromatography investigates composition of polyunsaturated fatty acids of membrane of erythrocytes, discharged of navel bloods neonatal from mothers who have transferred in the season gestation exacerbation herpes of an infection contamination and his influence on microviscosity of membrane. Essential infringements of data exchange of bonds in navel bloods neonatal with an exacerbation  herpes  infection  contaminations  (antiserum  capacity  IgG  to  virus  of  simple  herpes  of  1  type 1 : 12 800) which show deficiency essential ω-3 acids at simultaneous augmentation of the precursor proinflammatory eicosanoid ω-6 arachidonic acids, promoting augmentation of relative microviscosity of membrane of erythrocytes that will be one of probable causes of development of hypoxia are found.

Obtaining and Characterization of Alhagi persarum Boiss. et Buhse Callus Cell Cultures, Isoflavonoid Producers

2020 ◽  
Vol 36 (6) ◽  
pp. 35-48
Author(s):  
D.V. Коchkin ◽  
G.I. Sobolkovа ◽  
А.А. Fоmеnkov ◽  
R.А. Sidorov ◽  
А.М. Nоsоv
Keyword(s):  
Fatty Acids ◽  
Cell Culture ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Cell Cultures ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Gas Liquid Chromatography ◽  
Callus Cell

The physiological characteristics of the callus cell cultures of Alhagi persarum Boiss et Buhse, a member of the legume family, widely used in folk medicine, have been studied. It was shown that the source of the explant was an important factor in the initiation of callusogenesis: more intense callusogenesis (almost 100%) was observed for explants from various organs of sterile seedlings, rather than intact plants (less than 30%). As a result, more than 20 lines of morphologically different callus cell cultures were obtained, and the growth parameters for the 5 most intensively growing lines were determined. The composition of fatty acids (FA) of total lipids and secondary metabolites in the most physiologically stable callus line Aр-207 was analyzed. Using capillary gas-liquid chromatography with mass spectrometric detection (GLC-MS), 19 individual C12--C24 FAs were identified, the main fraction of which were palmitic (~ 23%), stearic (~ 22%), linoleic (~ 14%) and α-linolenic (~ 33%) acids. The established atypical ratio of FAs (a simultaneous high content of both saturated FAs and polyunsaturated α-linolenic acid) is possibly due to the adaptation of cells to in vitro growth conditions. Phytochemical analysis of the secondary metabolites was carried out using ultra-performance liquid chromatography with electrospray ionization mass spectrometric detection (UPLC MS). Compounds belonging to different structural groups of isoflavones were found. Aglycones (calycosin, formononetin and afrormosin isomer), glucosides (formononetin glucoside), as well as esters of glucosides (malonylglycosides of calicosin, formononetin, afrormosin isomers, glycitein and genistein) were detected. These secondary metabolites are widespread in plants of the Fabaceae family; however, isoflavones are rare in representatives of the Alhagi genus. The presence of malonylated isoflavone glycosides in Alhagi spp. was shown for the first time. endemic plant species, Alhagi, in vitro cell culture, callus cell culture, isoflavones, fatty acids All studies were carried out using the equipment of the "Experimental Biotechnological Facility" and the "All-Russian Collection of Cell Cultures of Higher Plants" of IРР RAS. This work was supported by the Russian Foundation for Basic Research (RFBR), contract no.18-54-06021 (Az_a), and the Government of the Russian Federation, Megagrant Project no. 075-15-2019-1882.

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