Determination of phytic acid in rapeseed meal

1980 ◽  
Vol 31 (7) ◽  
pp. 651-656 ◽  
Author(s):  
Bengt Uppström ◽  
Rita Svensson
Keyword(s):  
Phytic Acid ◽  
Rapeseed Meal

Determination of phytic acid and its degradation products in extracts of rape seeds and rapeseed meal

1995 ◽  
Vol 18 (4) ◽  
pp. 267-268 ◽  
Author(s):  
Bertrand Matthäus ◽  
Renate Lösing ◽  
Hans-Jochen Fiebig
Keyword(s):  
Phytic Acid ◽  
Degradation Products ◽  
Rapeseed Meal

scholarly journals The Determination of the Moisture of Rapeseed, Rapeseed Meal, and Soybean Meal, and the Oil Content of Rapeseed

1984 ◽  
Vol 33 (4) ◽  
pp. 194-199
Author(s):  
Michio YAMASHITA ◽  
Yoshiaki MACHIDA ◽  
Kiyoji SUZUKI ◽  
Haruo HIRAYAMA ◽  
Shosuke FUKUDA
Keyword(s):  
Soybean Meal ◽  
Oil Content ◽  
Rapeseed Meal

New spectrophotometric method for the determination of hemoglobin A1 compared with a microcolumn technique.

1982 ◽  
Vol 28 (1) ◽  
pp. 96-99 ◽  
Author(s):  
O Wålinder ◽  
G Ronquist ◽  
P J Fager
Keyword(s):  
Phytic Acid ◽  
Spectrophotometric Method ◽  
Healthy Subjects ◽  
Large Scale ◽  
Glycosylated Hemoglobin ◽  
Standard Curve ◽  
Sugar Moiety ◽  
Hemoglobin A1 ◽  
The Mean

Abstract We compared a spectrophotometric kit method (Glycospec) for determination of glycosylated hemoglobin (HbA1) with a microcolumn kit method (Bio-Rad). The Glycospec method is based on the change in absorbance when phytic acid binds to hemoglobin A. With glycosylated hemoglobin there is no such change because the binding is blocked by the sugar moiety. Inter-assay CVs were 2-6% for both methods. In healthy subjects the mean (+/- SD) value for HbAl was about 1% higher with the spectrophotometric than the microcolumn method. For samples from 122 diabetics the correlation between values for HbAl obtained with the two methods was acceptable (r = 0.89), although the spectrophotometric technique yielded 2-4% higher values, a difference at least partly due to the absence of 2,3-diphosphoglycerate from the spectrophotometric standards. Adding 1.8 mmol of it per liter to these standards caused displacement of the standard curve; HbAl values then agreed well with those of the microcolumn method. The spectrophotometric procedure is easily automated, and therefore is well suited for large-scale analyses if problems with standards and calibration can be solved.

scholarly journals Optimization of the Treatment of Wheat Samples for the Determination of Phytic Acid by HPLC with Refractive Index Detection

2009 ◽  
Vol 92 (3) ◽  
pp. 873-878 ◽  
Author(s):  
Rosa Amaro ◽  
Miguel Murillo ◽  
Zurima Gonzlez ◽  
András Escalona ◽  
Lus Hernández
Keyword(s):  
Refractive Index ◽  
High Performance Liquid Chromatography ◽  
Phytic Acid ◽  
High Performance ◽  
Substantial Improvement ◽  
Significant Loss ◽  
Phytic Acid Content ◽  
Extraction Step ◽  
Refractive Index Detection

Abstract The treatment of wheat samples was optimized before the determination of phytic acid by high-performance liquid chromatography with refractive index detection. Drying by lyophilization and oven drying were studied; drying by lyophilization gave better results, confirming that this step is critical in preventing significant loss of analyte. In the extraction step, washing of the residue and collection of this water before retention of the phytates in the NH2 Sep-Pak cartridge were important. The retention of phytates in the NH2 Sep-Pak cartridge and elimination of the HCl did not produce significant loss (P = 0.05) in the phytic acid content of the sample. Recoveries of phytic acid averaged 91, which is a substantial improvement with respect to values reported by others using this methodology.

Determination of phytic acid by complexometric titration of excess of iron(III)

The Analyst ◽  
1982 ◽  
Vol 107 (1281) ◽  
pp. 1503 ◽  
Author(s):  
R. Garcia-Villanova ◽  
R. J. Garcia-Villanova ◽  
C. Ruiz de Lope
Keyword(s):  
Phytic Acid ◽  
Complexometric Titration
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