The determination of lignin in plant tissues : Use of a continuous-extraction method to remove interfering materials

1954 ◽  
Vol 5 (2) ◽  
pp. 103-107
Author(s):  
D. Macdougall
Keyword(s):  
Extraction Method ◽  
Plant Tissues ◽  
Continuous Extraction

A STUDY OF METHODS FOR THE EXTRACTION OF NITROGENOUS MATERIAL FROM PLANTS WITH PARTICULAR REFERENCE TO SUBSEQUENT DETERMINATION OF THE LIGNIN CONTENT

1948 ◽  
Vol 26b (6) ◽  
pp. 457-463 ◽  
Author(s):  
W. A. DeLong ◽  
D. MacDougall
Keyword(s):  
Hydrochloric Acid ◽  
Extraction Method ◽  
Lignin Content ◽  
Mineral Acid ◽  
Plant Tissues ◽  
Acid Extraction ◽  
Continuous Extraction ◽  
Saturated Water ◽  
Subsequent Determination

A study has been made of methods for the removal of nitrogenous constituents from plant tissues prior to determination of their lignin contents. It was found that substitution of a continuous extraction method for the usual treatment with hot 1% hydrochloric acid had little effect on the amount of lignin isolated but apparently lowered its methoxyl content. Continuous extraction thus appears to cause some demethoxylation of lignin. Treatment with cold 5% acetic acid apparently can be substituted for the hot 1% hydrochloric acid extraction with very young but not with older, undried tissue. Ether saturated water was found to be the most satisfactory nonacid extractant for removal of nitrogen-containing material from young undried tissues. Upwards of 90% of the original nitrogen can be removed from such material by three extractions with this solvent. With older tissues or with material that has been dried, this proportion of the nitrogen cannot be removed except by extraction with hot dilute mineral acid.

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