Characterisation of the casein fraction of Ibérico cheese by electrophoretic techniques

Elena Molina; Mercedes Ramos; Lourdes Amigo

doi:10.1002/jsfa.1187

Human Blood Platelet Membrane Glycoproteins

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657050 ◽

1979 ◽

Vol 42 (05) ◽

pp. 1490-1502 ◽

Cited By ~ 3

Author(s):

C S P Jenkins ◽

E F Ali-Briggs ◽

G T E Zonneveld ◽

A Sturk ◽

J Clemetson

Keyword(s):

Specific Problem ◽

Sodium Dodecyl ◽

Normal Subjects ◽

Blood Platelet ◽

Platelet Membrane ◽

Buffer System ◽

Membrane Glycoprotein ◽

Membrane Glycoproteins ◽

Human Blood Platelet ◽

Electrophoretic Techniques

SummaryThe separation of the major platelet membrane glycoproteins of normal subjects and subjects with well defined platelet membrane glycoprotein abnormalities have been examined using four different polyacrylamide gel electrophoretic techniques (continuous and discontinuous). The mobilities of the resolved glycoprotein bands have been correlated with the glycoprotein nomenclature proposed for the conventional sodium dodecyl sulphate- phosphate buffer system. Since the glycoprotein distribution varies depending on the system used, the merits of each method should be considered before application to a specific problem.

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Recent Advances in Chiral Analysis of Proteins and Peptides

Separations ◽

10.3390/separations8080112 ◽

2021 ◽

Vol 8 (8) ◽

pp. 112

Author(s):

Marine Morvan ◽

Ivan Mikšík

Keyword(s):

Amino Acids ◽

Stationary Phases ◽

Enzymatic Digestion ◽

Chiral Separations ◽

Biological Compounds ◽

Health And Disease ◽

Electrophoretic Techniques ◽

A Site ◽

Future Work ◽

Proteins And Peptides

Like many biological compounds, proteins are found primarily in their homochiral form. However, homochirality is not guaranteed throughout life. Determining their chiral proteinogenic sequence is a complex analytical challenge. This is because certain D-amino acids contained in proteins play a role in human health and disease. This is the case, for example, with D-Asp in elastin, β-amyloid and α-crystallin which, respectively, have an action on arteriosclerosis, Alzheimer's disease and cataracts. Sequence-dependent and sequence-independent are the two strategies for detecting the presence and position of D-amino acids in proteins. These methods rely on enzymatic digestion by a site-specific enzyme and acid hydrolysis in a deuterium or tritium environment to limit the natural racemization of amino acids. In this review, chromatographic and electrophoretic techniques, such as LC, SFC, GC and CE, will be recently developed (2018–2020) for the enantioseparation of amino acids and peptides. For future work, the discovery and development of new chiral stationary phases and derivatization reagents could increase the resolution of chiral separations.

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Chromatographic and electrophoretic techniques, Vol. II, Zone electrophoresis, edited by I. Smith, William Heinemann, London, 4th ed., 1976, IX + 485 pp., price £ 9.00, ISBN 0-433-30505-5.

Journal of Chromatography B Biomedical Sciences and Applications ◽

10.1016/s0378-4347(00)81387-0 ◽

1978 ◽

Vol 145 (3) ◽

pp. 513-514

Keyword(s):

Zone Electrophoresis ◽

Electrophoretic Techniques

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Development of Experimental and Computational Techniques to Improve or Predict the Likelihood of Separation Success of Chromatographic and Electrophoretic Techniques

10.17918/etd-7026 ◽

2021 ◽

Author(s):

Erin Joan Ennis

Keyword(s):

Computational Techniques ◽

Electrophoretic Techniques

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The effects of Acute and Chronic β-Adrenoceptor Blockade on the Myofibrillar Contractile System in the Dog Heart

Clinical Science ◽

10.1042/cs0670259 ◽

1984 ◽

Vol 67 (2) ◽

pp. 259-267 ◽

Cited By ~ 2

Author(s):

M. K. Davies ◽

P. Cummins ◽

W. A. Littler

Keyword(s):

Calcium Sensitivity ◽

Maximal Activity ◽

Biopsy Specimens ◽

Contractile System ◽

Chronic Therapy ◽

Before And After ◽

Adaptive Effect ◽

Electrophoretic Techniques ◽

Myocardial Contractile ◽

And Function

1. Electrophoretic and enzyme techniques have been used to study the structure and function of the contractile protein system in the myocardium of dogs before and after β-adrenoceptor blockade. Animals were examined after acute β-adrenoceptor blockade by using intravenous atenolol (0.2 mg/kg) and following chronic therapy with oral atenolol (100 mg twice daily) for a mean period of 106 days. 2. Two-dimensional polyacrylamide-gel electrophoretic techniques were used to examine the myocardial contractile and regulatory proteins present in endomyocardial biopsy specimens obtained after acute and chronic β-adrenoceptor blockade. No differences in charge, molecular weight or the relative proportions of actin, myosin light chains, tropomyosin or troponin-C were seen after either acute or chronic β-adrenoceptor blockade. 3. The maximal activity and calcium sensitivity of the myofibrillar adenosine triphosphatase (ATPase) was also unchanged after acute and chronic atenolol therapy. 4. It is concluded that β-adrenoceptor blockade has no significant adaptive effect on the structural or functional properties of the myofibril.

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