Do formalin fixation and freeze-thaw affect near-infrared Raman spectroscopy of cartilaginous tissue? A preliminary ex vivo analysis of native human articular cartilage

2015 ◽  
Vol 46 (11) ◽  
pp. 1166-1172 ◽  
Author(s):  
Yasuhito Takahashi ◽  
Takaaki Shishido ◽  
Kengo Yamamoto ◽  
Yasunobu Sawaji ◽  
Jun Nishida ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Articular Cartilage ◽  
Near Infrared ◽  
Ex Vivo ◽  
Formalin Fixation ◽  
Human Articular Cartilage ◽  
Cartilaginous Tissue ◽  
Freeze Thaw

scholarly journals Optical Biomarkers for the Diagnosis of Osteoarthritis through Raman Spectroscopy: Radiological and Biochemical Validation Using Ex Vivo Human Cartilage Samples

Diagnostics ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 546
Author(s):  
Paula Casal-Beiroa ◽  
Vanesa Balboa-Barreiro ◽  
Natividad Oreiro ◽  
Sonia Pértega-Díaz ◽  
Francisco J. Blanco ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Articular Cartilage ◽  
Ex Vivo ◽  
Cartilage Degradation ◽  
Calcium Pyrophosphate ◽  
Calcium Pyrophosphate Dihydrate ◽  
Label Free ◽  
Molecular Fingerprint ◽  
Human Cartilage ◽  
Biochemical Validation

Osteoarthritis (OA) is the most common rheumatic disease, characterized by progressive articular cartilage degradation. Raman spectroscopy (RS) has been recently proposed as a label-free tool to detect molecular changes in musculoskeletal tissues. We used cartilage samples derived from human femoral heads to perform an ex vivo study of different Raman signals and ratios, related to major and minor molecular components of articular cartilage, hereby proposed as candidate optical biomarkers for OA. Validation was performed against the radiological Kellgren–Lawrence (K-L) grading system, as a gold standard, and cross-validated against sulfated glycosaminoglycans (sGAGs) and total collagens (Hyp) biochemical contents. Our results showed a significant decrease in sGAGs (SGAGs, A1063 cm−1/A1004 cm−1) and proteoglycans (PGs, A1375 cm−1/A1004 cm−1) and a significant increase in collagen disorganization (ColD/F, A1245 cm−1/A1270 cm−1), with OA severity. These were correlated with sGAGs or Hyp contents, respectively. Moreover, the SGAGs/HA ratio (A1063 cm−1/A960 cm−1), representing a functional matrix, rich in proteoglycans, to a mineralized matrix-hydroxyapatite (HA), was significantly lower in OA cartilage (K-L I vs. III–IV, p < 0.05), whilst the mineralized to collagenous matrix ratio (HA/Col, A960 cm−1/A920 cm−1) increased, being correlated with K-L. OA samples showed signs of tissue mineralization, supported by the presence of calcium crystals-related signals, such as phosphate, carbonate, and calcium pyrophosphate dihydrate (MGP, A960 cm−1/A1004 cm−1, MGC, A1070 cm−1/A1004 cm−1 and A1050 cm−1/A1004 cm−1). Finally, we observed an increase in lipids ratio (IL, A1450 cm−1/A1670 cm−1) with OA severity. As a conclusion, we have described the molecular fingerprint of hip cartilage, validating a panel of optical biomarkers and the potential of RS as a complementary diagnostic tool for OA.

scholarly journals Applications of Vibrational Spectroscopy for Analysis of Connective Tissues

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 922
Author(s):  
William Querido ◽  
Shital Kandel ◽  
Nancy Pleshko
Keyword(s):  
Raman Spectroscopy ◽  
Vibrational Spectroscopy ◽  
Near Infrared ◽  
Ex Vivo ◽  
Biological Tissues ◽  
Label Free ◽  
Connective Tissues ◽  
Bone Properties ◽  
Composition And Structure

Advances in vibrational spectroscopy have propelled new insights into the molecular composition and structure of biological tissues. In this review, we discuss common modalities and techniques of vibrational spectroscopy, and present key examples to illustrate how they have been applied to enrich the assessment of connective tissues. In particular, we focus on applications of Fourier transform infrared (FTIR), near infrared (NIR) and Raman spectroscopy to assess cartilage and bone properties. We present strengths and limitations of each approach and discuss how the combination of spectrometers with microscopes (hyperspectral imaging) and fiber optic probes have greatly advanced their biomedical applications. We show how these modalities may be used to evaluate virtually any type of sample (ex vivo, in situ or in vivo) and how “spectral fingerprints” can be interpreted to quantify outcomes related to tissue composition and quality. We highlight the unparalleled advantage of vibrational spectroscopy as a label-free and often nondestructive approach to assess properties of the extracellular matrix (ECM) associated with normal, developing, aging, pathological and treated tissues. We believe this review will assist readers not only in better understanding applications of FTIR, NIR and Raman spectroscopy, but also in implementing these approaches for their own research projects.

Assessment of ex-vivo and in-vivo near-infrared Raman spectroscopy for the classification of dysplasia within Barrett's esophagus

2000 ◽  
Author(s):  
Martin G. Shim ◽  
Louis-Michel Wong Kee Song ◽  
Norman E. Marcon ◽  
Shirley Hassaram ◽  
Brian C. Wilson
Keyword(s):  
Raman Spectroscopy ◽  
Barrett’S Esophagus ◽  
Barrett's Esophagus ◽  
Near Infrared ◽  
Ex Vivo

Near-Infrared Multichannel Raman Spectroscopy with a 1064 nm Excitation Wavelength for Ex Vivo Diagnosis of Gastric Cancer

2011 ◽  
Vol 169 (2) ◽  
pp. e137-e143 ◽  
Author(s):  
Toshiki Kawabata ◽  
Hirotoshi Kikuchi ◽  
Shigetoshi Okazaki ◽  
Masayoshi Yamamoto ◽  
Yoshihiro Hiramatsu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Raman Spectroscopy ◽  
Near Infrared ◽  
Ex Vivo ◽  
Excitation Wavelength

scholarly journals Progenitor Cells Activated by Platelet Lysate in Human Articular Cartilage as a Tool for Future Cartilage Engineering and Reparative Strategies

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 1052 ◽  
Author(s):  
Simonetta Carluccio ◽  
Daniela Martinelli ◽  
Maria Elisabetta Federica Palamà ◽  
Rui Cruz Pereira ◽  
Roberto Benelli ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Progenitor Cells ◽  
Articular Chondrocytes ◽  
Ex Vivo ◽  
Primary Cultures ◽  
Platelet Lysate ◽  
Cartilage Explants ◽  
Cartilage Tissue ◽  
Human Articular Cartilage

Regenerative strategies for human articular cartilage are still challenging despite the presence of resident progenitor cell population. Today, many efforts in the field of regenerative medicine focus on the use of platelet derivatives due to their ability to reactivate endogenous mechanisms supporting tissue repair. While their use in orthopedics continues, mechanisms of action and efficacy need further characterization. We describe that the platelet lysate (PL) is able to activate chondro-progenitor cells in a terminally differentiated cartilage tissue. Primary cultures of human articular chondrocytes (ACs) and cartilage explants were set up from donor hip joint biopsies and were treated in vitro with PL. PL recruited a chondro-progenitors (CPCs)-enriched population from ex vivo cartilage culture, that showed high proliferation rate, clonogenicity and nestin expression. CPCs were positive for in vitro tri-lineage differentiation and formed hyaline cartilage-like tissue in vivo without hypertrophic fate. Moreover, the secretory profile of CPCs was analyzed, together with their migratory capabilities. Some CPC-features were also induced in PL-treated ACs compared to fetal bovine serum (FBS)-control ACs. PL treatment of human articular cartilage activates a stem cell niche responsive to injury. These facts can improve the PL therapeutic efficacy in cartilage applications.

Effect of formalin fixation on the near-infrared Raman spectroscopy of normal and cancerous human bronchial tissues

2003 ◽  
Author(s):  
Zhiwei Huang ◽  
Annette McWilliams ◽  
Stephen Lam ◽  
John English ◽  
David McLean ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Near Infrared ◽  
Formalin Fixation

First ex vivo study demonstrating that 99mTc-NTP 15-5 radiotracer binds to human articular cartilage

2011 ◽  
Vol 38 (11) ◽  
pp. 2077-2082 ◽  
Author(s):  
Florent Cachin ◽  
Stéphane Boisgard ◽  
Aurélien Vidal ◽  
Marc Filaire ◽  
Philippe Auzeloux ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Ex Vivo ◽  
Human Articular Cartilage ◽  
Ex Vivo Study

scholarly journals Comparison of hip joint cartilage degeneration assessed by histology and ex vivo optical coherence tomography

2014 ◽  
Vol 6 (2) ◽  
Author(s):  
Hakan Pilge ◽  
Klaudia Huber-van der Velden ◽  
Monika Herten ◽  
Sabine Kurzidem ◽  
Rüdiger Krauspe ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Articular Cartilage ◽  
Histological Analysis ◽  
Ex Vivo ◽  
Cartilage Degeneration ◽  
Observer Agreement ◽  
Histological Evaluation ◽  
Human Articular Cartilage ◽  
Optical Coherence ◽  
Interclass Correlation

The aim of this study is to validate optical coherence tomography (OCT) in assessing human articular cartilage by means of histological analyses. Twenty resected human femoral head specimens were evaluated with OCT and histological analysis. OCT and histological evaluation was performed according to the Bear and the Mankin criteria. OCT grades and Mankin scores (total score and sub-score structure) were correlated and intra-/inter-observer agreement for repeated OCT evaluations was tested by interclass-correlation coefficient (ICC) analysis. OCT grades and Mankin scores were correlated [Spearman correlation = 0.742 (total) and 0.656 (structure), P&lt;0.001], revealing significant differences between the histological scores in various OCT grades of cartilage degeneration (P&lt;0.001). Intra-observer (ICC 0.930) and inter-observer (ICC 0.933) reliability was high (P&lt;0.001). OCT appears to be reliable in the assessment of human articular cartilage. Further studies on intra-operative cartilage evaluation by OCT are necessary to substantiate its applicability in clinical routine.

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