Development of a sensitive, stable and EGFR-specific molecular imaging agent for surface enhanced Raman spectroscopy

2015 ◽  
Vol 46 (5) ◽  
pp. 434-446 ◽  
Author(s):  
Leanne J. Lucas ◽  
Claudio Tellez ◽  
Maiara L. Castilho ◽  
Christopher L. D. Lee ◽  
Michael A. Hupman ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Molecular Imaging ◽  
Surface Enhanced Raman Spectroscopy ◽  
Imaging Agent ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Molecular Imaging Agent

scholarly journals Molecular imaging of atherosclerosis: spotlight on Raman spectroscopy and surface-enhanced Raman scattering

Heart ◽  
2017 ◽  
Vol 104 (6) ◽  
pp. 460-467 ◽  
Author(s):  
Neil MacRitchie ◽  
Gianluca Grassia ◽  
Jonathan Noonan ◽  
Paul Garside ◽  
Duncan Graham ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Molecular Imaging ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Photon Emission ◽  
Surface Enhanced Raman Spectroscopy ◽  
Emission Tomography ◽  
Good Spatial Resolution ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

To accurately predict atherosclerotic plaque progression, a detailed phenotype of the lesion at the molecular level is required. Here, we assess the respective merits and limitations of molecular imaging tools. Clinical imaging includes contrast-enhanced ultrasound, an inexpensive and non-toxic technique but with poor sensitivity. CT benefits from high spatial resolution but poor sensitivity coupled with an increasing radiation burden that limits multiplexing. Despite high sensitivity, positron emission tomography and single-photon emission tomography have disadvantages when applied to multiplex molecular imaging due to poor spatial resolution, signal cross talk and increasing radiation dose. In contrast, MRI is non-toxic, displays good spatial resolution but poor sensitivity. Preclinical techniques include near-infrared fluorescence (NIRF), which provides good spatial resolution and sensitivity; however, multiplexing with NIRF is limited, due to photobleaching and spectral overlap. Fourier transform infrared spectroscopy and Raman spectroscopy are label-free techniques that detect molecules based on the vibrations of chemical bonds. Both techniques offer fast acquisition times with Raman showing superior spatial resolution. Raman signals are inherently weak; however, leading to the development of surface-enhanced Raman spectroscopy (SERS) that offers greatly increased sensitivity due to using metallic nanoparticles that can be functionalised with biomolecules targeted against plaque ligands while offering high multiplexing potential. This asset combined with high spatial resolution makes SERS an exciting prospect as a diagnostic tool. The ongoing refinements of SERS technologies such as deep tissue imaging and portable systems making SERS a realistic prospect for translation to the clinic.

scholarly journals Molecular imaging with surface-enhanced Raman spectroscopy nanoparticle reporters

MRS Bulletin ◽  
2013 ◽  
Vol 38 (8) ◽  
pp. 625-630 ◽  
Author(s):  
Jesse V. Jokerst ◽  
Christoph Pohling ◽  
Sanjiv S. Gambhir
Keyword(s):  
Raman Spectroscopy ◽  
Molecular Imaging ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Image Position

Abstract

scholarly journals In vivo multiplex molecular imaging of vascular inflammation using surface-enhanced Raman spectroscopy

Theranostics ◽  
2018 ◽  
Vol 8 (22) ◽  
pp. 6195-6209 ◽  
Author(s):  
Jonathan Noonan ◽  
Steven M. Asiala ◽  
Gianluca Grassia ◽  
Neil MacRitchie ◽  
Kirsten Gracie ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Molecular Imaging ◽  
Vascular Inflammation ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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