SERS active Ag nanoparticles in mesoporous silicon: detection of organic molecules and peptide-antibody assays

2011 ◽  
Vol 43 (6) ◽  
pp. 730-736 ◽  
Author(s):  
Alessandro Virga ◽  
Paola Rivolo ◽  
Emiliano Descrovi ◽  
Alessandro Chiolerio ◽  
Gabriella Digregorio ◽  
...  
Keyword(s):  
Ag Nanoparticles ◽  
Organic Molecules ◽  
Mesoporous Silicon ◽  
Peptide Antibody ◽  
Antibody Assays

scholarly journals Organic Molecule Detection Based on SERS in Microfluidics

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Xin Zhang ◽  
Haiyan Zhang ◽  
Sheng Yan ◽  
Zugang Zeng ◽  
Anshou Huang ◽  
...  
Keyword(s):  
Environmental Monitoring ◽  
Ag Nanoparticles ◽  
Organic Molecule ◽  
Organic Molecules ◽  
Chemical Reduction ◽  
Fdtd Method ◽  
Great Influence ◽  
High Sensitivity ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sers Substrate

AbstractSensitive in situ detection of organic molecules is highly demanded in environmental monitoring. In this work, the surface enhanced Raman spectroscopy (SERS) is adopted in microfluidics to detect the organic molecules with high accuracy and high sensitivity. Here the SERS substrate in microchannel consists of Ag nanoparticles synthesized by chemical reduction. The data indicates the fabrication conditions have great influence on the sizes and distributions of Ag nanoparticles, which play an important role on the SERS enhancement. This result is further confirmed by the simulation of electromagnetic field distributions based on finite difference time domain (FDTD) method. Furthermore, the SERS spectra of organic molecule (methylene blue) obtained in this plasmonic microfluidic system exhibit good reproducibility with high sensitivity. By a combination of SERS and microfluidics, our work not only explores the research field of plasmonics but also has broad application prospects in environmental monitoring.

scholarly journals Anti-citrullinated peptide antibody assays and their role in the diagnosis of rheumatoid arthritis

2009 ◽  
Vol 61 (11) ◽  
pp. 1472-1483 ◽  
Author(s):  
Rohit Aggarwal ◽  
Katherine Liao ◽  
Raj Nair ◽  
Sarah Ringold ◽  
Karen H. Costenbander
Keyword(s):  
Rheumatoid Arthritis ◽  
Peptide Antibody ◽  
Antibody Assays ◽  
Citrullinated Peptide

scholarly journals Comparison of nine second- and third-generation anti-cyclic citrullinated peptide antibody assays for the diagnosis of rheumatoid arthritis

2013 ◽  
Vol 36 (2) ◽  
pp. 104-114
Author(s):  
Nobuhide HAYASHI ◽  
Shunichi KUMAGAI ◽  
Kenichiro ONUMA ◽  
Kenichi UTO ◽  
Daisuke SUGIYAMA ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Third Generation ◽  
Cyclic Citrullinated Peptide ◽  
Peptide Antibody ◽  
Antibody Assays ◽  
Citrullinated Peptide

scholarly journals Multi-peptide ELISAs overcome cross-reactivity and inadequate sensitivity of conventional Chlamydia pneumoniae serology

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kh Shamsur Rahman ◽  
Bernhard Kaltenboeck
Keyword(s):  
Chlamydia Pneumoniae ◽  
High Specificity ◽  
Cross Reactivity ◽  
Peptide Antigens ◽  
Specificity And Sensitivity ◽  
Peptide Antibody ◽  
Antibody Assays ◽  
Human Sera ◽  
Composite Reference Standard ◽  
Individual Peptide

Abstract Cross-reactivity of classical chlamydial antigens compromises Chlamydia (C.) pneumoniae serology. By testing with 185 human antisera, we expanded 18 previously discovered C. pneumoniae-specific B-cell epitopes to 48 peptide antigens from 12 C. pneumoniae immunodominant proteins. For specific detection of antibodies against C. pneumoniae, we developed novel ELISAs with strongly reactive individual peptide antigens and mixtures of these peptides. By comparison to a composite reference standard (CRS) for anti-C. pneumoniae antibody status of human sera, the top-performing CpnMixF12 peptide assay showed 91% sensitivity at 95% specificity, significantly higher than 4 commercial anti-C. pneumoniae IgG ELISAs (36-12% sensitivity at 95% specificity). Human C. pneumoniae (Cpn) and C. trachomatis (Ctr) seroreactivity was 54% biased towards co-positivity in commercial Cpn and Ctr ELISAs, but unbiased in Cpn and Ctr peptide antibody assays, suggesting severe cross-reactivity of commercial ELISAs. Using hyperimmune mouse sera against each of 11 Chlamydia spp., we confirm that commercial Cpn and Ctr ELISA antigens are cross-reactive among all Chlamydia spp., but Cpn and Ctr peptide antigens react only with antisera against the cognate chlamydial species. With simultaneously high specificity and sensitivity, and convenient use for non-specialized laboratories, these ELISAs have the potential to improve serodiagnosis of C. pneumoniae infection.

Diagnostic Utility of Modified Gliadin Peptide Antibody Assays in New Zealand Children

2013 ◽  
Vol 57 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Benjamin C. Hope ◽  
Rohan Ameratunga ◽  
Paul M. Austin ◽  
Helen M. Evans ◽  
Jeannette MacFarlane ◽  
...  
Keyword(s):  
New Zealand ◽  
Diagnostic Utility ◽  
Peptide Antibody ◽  
Antibody Assays ◽  
Gliadin Peptide

Diagnostic performance of IgG anti-deamidated gliadin peptide antibody assays is comparable to IgA anti-tTG in celiac disease

2010 ◽  
Vol 411 (13-14) ◽  
pp. 931-935 ◽  
Author(s):  
Pieter Vermeersch ◽  
Karel Geboes ◽  
Godelieve Mariën ◽  
Ilse Hoffman ◽  
Martin Hiele ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Diagnostic Performance ◽  
Peptide Antibody ◽  
Antibody Assays ◽  
Gliadin Peptide

scholarly journals Performance characteristics of rheumatoid factor and anti-cyclic citrullinated peptide antibody assays may impact ACR/EULAR classification of rheumatoid arthritis

2018 ◽  
Vol 77 (5) ◽  
pp. 667-677 ◽  
Author(s):  
Lieve Van Hoovels ◽  
Julie Jacobs ◽  
Bert Vander Cruyssen ◽  
Stefanie Van den Bremt ◽  
Patrick Verschueren ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Characteristic Analysis ◽  
Performance Impact ◽  
American College Of Rheumatology ◽  
European League Against Rheumatism ◽  
Peptide Antibody ◽  
Antibody Assays ◽  
Antibody Levels ◽  
First Time

ObjectivesRheumatoid factor (RF) and anti-cyclic citrullinated protein/peptide antibodies (ACPA) are integrated in the 2010 American College of Rheumatology/European League Against Rheumatism (ACR/EULAR) classification criteria for rheumatoid arthritis (RA). The objectives of this study were to evaluate the technical and diagnostic performance of different RF and ACPA assays and to evaluate whether differences in performance impact RA classification.MethodsSamples from 594 consecutive patients who for the first time consulted a rheumatologist (44 of whom were diagnosed with RA) and 26 extra newly diagnosed patients with RA were analysed with six different RF assays (Menarini, Thermo Fisher, Inova, Roche, Abbott, Euroimmun) and seven different ACPA assays (Menarini, Thermo Fisher, Inova, Roche, Abbott, Euro Diagnostica, Euroimmun).ResultsWe found differences in analytical performance between assays. There was poor numerical agreement between the different RF and ACPA assays. For all assays, the likelihood ratio for RA increased with increasing antibody levels. The areas under the curve of receiver operating characteristic analysis of the RF (range 0.676–0.709) and ACPA assays (range 0.672–0.769) only differed between some ACPA assays. Nevertheless, using the cut-off proposed by the manufacturer, there was a large variation in sensitivity and specificity between assays (mainly for RF). Consequently, depending on the assay used, a subgroup of patients (13% for RF, 1% for ACPA and 9% for RF/ACPA) might or might not be classified as RA according to the 2010 ACR/EULAR criteria.ConclusionDue to poor harmonisation of RF and ACPA assays and of test result interpretation, RA classification according to 2010 ACR/EULAR criteria may vary when different assays are used.

Sign in / Sign up

Export Citation Format

Share Document