Analysis of Multicomponent Formulations Containing Pseudoephedrine HCI and Chlorpheniramine Maleate Using First-Derivative Spectroscopy on a Diode-Array Spectrophotometer

1987 ◽  
Vol 76 (3) ◽  
pp. 242-244 ◽  
Author(s):  
James M. Hoover ◽  
Richard A. Soltero ◽  
Padam C. Bansal
Keyword(s):  
Diode Array ◽  
Chlorpheniramine Maleate ◽  
First Derivative ◽  
Derivative Spectroscopy ◽  
Diode Array Spectrophotometer

scholarly journals Simultaneous Determination of Losartan and Hydrochlorothiazide in Combined Dosage Forms by First-Derivative Spectroscopy and High-Performance Thin-Layer Chromatography

2001 ◽  
Vol 84 (6) ◽  
pp. 1715-1723 ◽  
Author(s):  
Shailesh A Shah ◽  
Ishwarsinh S Rathod ◽  
Bhanubhai N Suhagia ◽  
Shrinivas S Savale ◽  
Jignesh B Patel
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Correlation Coefficients ◽  
Tolerability Profile ◽  
Angiotensin Ii Receptor ◽  
First Derivative ◽  
Derivative Spectroscopy ◽  
Layer Chromatography ◽  
Hptlc Method

Abstract Losartan (LST) is the first orally active nonpeptide angiotensin-II receptor antagonist with an improved safety and tolerability profile. It is prescribed alone or in combination with hydrochlorothiazide (HCTZ) for the treatment of moderate-to-severe hypertension. This paper describes the development of 2 methods that use different techniques, first-derivative spectroscopy and high-performance thin-layer chromatography (HPTLC), to determine LST and HCTZ in the presence of each other. LST and HCTZ in combined preparations were quantitated by using the first-derivative responses at 271.6 nm for LST and 335.0 nm for HCTZ in spectra of their solutions in water. The linearity ranges are 30–70 μg/mL for LST and 7.5–17.5 μg/mL for HCTZ with correlation coefficients of 0.9998 and 0.9997, respectively. In the HPTLC method, a mobile phase of chloroform–methanol–acetone–formic acid (7.5 + 1.5 + 0.5 + 0.03, v/v) and a prewashed Silica Gel G60 F254 TLC plate as the stationary phase were used to resolve LST and HCTZ in a mixture. Two well-separated and sharp peaks for LST and HCTZ were obtained at Rf values of 0.61 ± 0.02 and 0.41 ± 0.02, respectively. LST and HCTZ were quantitated at 254.0 nm. The linearity ranges obtained for the HPTLC method are 400–1200 and 100–300 ng/spot with corresponding correlation coefficients of 0.9944 and 0.9979, for LST and HCTZ, respectively. Both methods were validated, and the results were compared statistically. They were found to be accurate, specific, and reproducible. The methods were successfully applied to the estimation of LST and HCTZ in combined tablet formulations.

Applications of a Vidicon Spectrometer to Analytical Problems in Clinical Chemistry

1975 ◽  
Vol 21 (9) ◽  
pp. 1192-1200 ◽  
Author(s):  
Harry L Pardue ◽  
Alan E McDowell ◽  
Douglas M Fast ◽  
Michael J Milano
Keyword(s):  
Lactate Dehydrogenase ◽  
Clinical Chemistry ◽  
Critical Evaluation ◽  
Clinical Analysis ◽  
Detector Response ◽  
First Derivative ◽  
Derivative Spectroscopy ◽  
Enzymatic Determination

Abstract This report discusses characteristics of a custom-designed vidicon spectrometer and evaluates its applicability to several clinical analysis problems. Data show that the vidicon detector response is linear with intensity over about four orders of magnitude and that the uncertainty in absorbance measurements can approach 0.001 absorbance units in the range from 0 to 2 absorbance units. Applications include the enzymatic determination of glucose, the determination of lactate dehydrogenase, and determinations of barbital, chlordiazepoxide, and glutethimide. Capabilities of the instrument system for first-derivative spectroscopy are also discussed. The discussion includes a critical evaluation of the potential advantages and limitations of the concept.

Precision of a diode-array spectrophotometer

1990 ◽  
Vol 234 ◽  
pp. 395-401 ◽  
Author(s):  
M. Blanco ◽  
J. Coello ◽  
H. Iturriaga ◽  
S. Maspoch ◽  
J. Riba
Keyword(s):  
Diode Array ◽  
Diode Array Spectrophotometer

A Portable Diode Array Spectrophotometer

2016 ◽  
Vol 70 (5) ◽  
pp. 874-878 ◽  
Author(s):  
David Stephenson
Keyword(s):  
Diode Array ◽  
Diode Array Spectrophotometer

The Fuge Tube Diode Array Spectrophotometer

2008 ◽  
Vol 85 (12) ◽  
pp. 1663
Author(s):  
B. T. Arneson ◽  
K. K. Stewart ◽  
J. J. Lagowski ◽  
S. R. Long
Keyword(s):  
Diode Array ◽  
Diode Array Spectrophotometer
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