scholarly journals Capric Acid Absorption in the Presence of Hydroxypropyl-β-Cyclodextrin in the Rat Ileum using the In Situ Single-Pass Perfusion Technique

2015 ◽  
Vol 104 (9) ◽  
pp. 2832-2844 ◽  
Author(s):  
Richard V. Hymas ◽  
Norman F.H. Ho ◽  
William I. Higuchi
Keyword(s):  
Capric Acid ◽  
Perfusion Technique ◽  
Rat Ileum ◽  
Single Pass ◽  
Acid Absorption ◽  
Pass Perfusion

A Systematic Examination of the In Vitro Ussing Chamber and the In Situ Single-Pass Perfusion Model Systems in Rat Ileum Permeation of Model Solutes

2003 ◽  
Vol 92 (2) ◽  
pp. 344-359 ◽  
Author(s):  
Keiko Tsutsumi ◽  
S.Kevin Li ◽  
Abdel-Halim Ghanem ◽  
Norman F.H. Ho ◽  
William I. Higuchi
Keyword(s):  
Ussing Chamber ◽  
Model Systems ◽  
Rat Ileum ◽  
Single Pass ◽  
Perfusion Model ◽  
Pass Perfusion ◽  
Systematic Examination

Assessment of Ferrous Glycinate Liposome Absorption Using in Situ Single-Pass Perfusion Model

2017 ◽  
Vol 13 (9) ◽  
Author(s):  
Baomiao Ding ◽  
Xiangzhou Yi ◽  
Li Li ◽  
Hualin Yang
Keyword(s):  
Particle Size ◽  
Phytic Acid ◽  
Intestinal Permeability ◽  
Iron Absorption ◽  
Inhibitory Effects ◽  
Single Pass ◽  
Pass Perfusion ◽  
Absorption Pathways ◽  
Main Factor

AbstractLiposomes could be employed to improve the absorption of iron. The purpose of this study was to estimate the intestinal permeability of ferrous glycinate liposomes and to assess the effects of phytic acid, zinc and particle size on iron absorption usingin situsingle-pass perfusion in rats. The results showed that the absorption of ferrous glycinate liposomes was obviously higher than that of ferrous glycinate. The inhibitory effects of phytic acid and zinc on iron absorption were reduced by incorporating ferrous glycinate into liposomes. The particle size of ferrous glycinate liposomes was also a main factor for affecting iron absorption, and the intestinal permeability of the liposomes decreased with its particle size increasing. The results suggested that liposomes could be a potent delivery system to decrease the inhibitory effects of phytic acid and zinc and to enhance iron absorption. Furthermore, liposomes could alter the absorption pathways of ferrous glycinate.

scholarly journals Regional Absorption of Fimasartan in the Gastrointestinal Tract by an Improved in situ Absorption Method in Rats

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 174 ◽  
Author(s):  
Tae Hwan Kim ◽  
Soo Heui Paik ◽  
Yong Ha Chi ◽  
Jürgen B. Bulitta ◽  
Da Young Lee ◽  
...  
Keyword(s):  
Small Intestine ◽  
Large Intestine ◽  
Great Promise ◽  
Absorption Method ◽  
Single Pass ◽  
Pass Perfusion ◽  
Starting Point ◽  
Fraction Absorbed ◽  
In Situ Absorption

The aim of the present study was to assess the regional absorption of fimasartan by an improved in situ absorption method in comparison with the conventional in situ single-pass perfusion method in rats. After each gastrointestinal segment of interest was identified, fimasartan was injected into the starting point of each segment and the unabsorbed fimasartan was discharged from the end point of the segment. Blood samples were collected from the jugular vein to evaluate the systemic absorption of the drug. The relative fraction absorbed (Fabs,relative) values in the specific gastrointestinal region calculated based on the area under the curve (AUC) values obtained after the injection of fimasartan into the gastrointestinal segment were 8.2% ± 3.2%, 23.0% ± 12.1%, 49.7% ± 11.5%, and 19.1% ± 11.9% for the stomach, duodenum, small intestine, and large intestine, respectively, which were comparable with those determined by the conventional in situ single-pass perfusion. By applying the fraction of the dose available at each gastrointestinal segment following the oral administration, the actual fraction absorbed (F’abs) values at each gastrointestinal segment were estimated at 10.9% for the stomach, 27.1% for the duodenum, 40.7% for the small intestine, and 5.4% for the large intestine, which added up to the gastrointestinal bioavailability (FX·FG) of 84.1%. The present method holds great promise to assess the regional absorption of a drug and aid to design new drug formulations.

HCO3 transport in rat jejunum: relationship to NaCl and H2O transport in vivo.

1977 ◽  
Vol 232 (1) ◽  
pp. E62
Author(s):  
R B Podesta ◽  
D F Mettrick
Keyword(s):  
Cation Exchange ◽  
Rat Jejunum ◽  
Perfusion Technique ◽  
Single Pass ◽  
Physiological Importance ◽  
Pass Perfusion ◽  
Layer Effect ◽  
Disequilibrium Ph ◽  
Precise Degree

HCO3- absorption and its association with Na+ absorption has been studied in the rat jejunum in vivo, using a single-pass perfusion technique. The method of disequilibrium pH, the only valid way of demonstrating jejunal H+ secretion, was used to distinguish between an HCO3- pump and H+ secretion as the mechanism of HCO3- absorption. HCO3- stimulated Na+ absorption; Na+ deletion inhibited HCO3 absorption, decreased luminal acidification, and decreased the level of luminal PCO2. These results confirmed an Na+:H+ cation exchange, the possible mechanism of which is discussed in terms of results using other tissues. Na+-dependent HCO3-absorption made up a larger part of total HCO3-absorption as the luminal HCO3-concentrations diminished, although the precise degree of Na+-dependency could not be determined because of the unstirred layer effect. The mechanism of Na+-independent HCO3-absorption was not established, but it was not affected by PD, Cl-, or H2O movements. Glucose-stimulated and HCO3-stimulated Na+ absorption were less than additive. The physiological importance of HCO3-stimulated Na+ absorption in the acidic postprandial jejunum is probably due entirely to the effect of free CO2 in the lumen.

scholarly journals Solvent drag in jejunal absorption of salicylic acid and antipyrine obtained by in situ single-pass perfusion method in rat.

1984 ◽  
Vol 7 (4) ◽  
pp. 246-253 ◽  
Author(s):  
TOYOTO HIRASAWA ◽  
TOSHIKO MURAOKA ◽  
AKIRA KARINO ◽  
MASAHIRO HAYASHI ◽  
SHOJI AWAZU
Keyword(s):  
Salicylic Acid ◽  
Solvent Drag ◽  
Jejunal Absorption ◽  
Single Pass ◽  
Perfusion Method ◽  
Pass Perfusion

scholarly journals INTESTINAL SINGLE-PASS IN SITU PERFUSION TECHNIQUE IN RAT: THE INFLUENCE OF L-CARNITINE ON ABSORPTION OF 7-METHOXYTACRINE

2005 ◽  
Vol 149 (2) ◽  
pp. 433-435 ◽  
Author(s):  
Martin Kunes ◽  
Zbynek Svoboda ◽  
Jaroslav Kvetina ◽  
Vladimir Herout ◽  
Josef Herink ◽  
...  
Keyword(s):  
Perfusion Technique ◽  
Single Pass ◽  
In Situ Perfusion
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