A Systematic Examination of the In Vitro Ussing Chamber and the In Situ Single-Pass Perfusion Model Systems in Rat Ileum Permeation of Model Solutes

2003 ◽  
Vol 92 (2) ◽  
pp. 344-359 ◽  
Author(s):  
Keiko Tsutsumi ◽  
S.Kevin Li ◽  
Abdel-Halim Ghanem ◽  
Norman F.H. Ho ◽  
William I. Higuchi
Keyword(s):  
Ussing Chamber ◽  
Model Systems ◽  
Rat Ileum ◽  
Single Pass ◽  
Perfusion Model ◽  
Pass Perfusion ◽  
Systematic Examination

scholarly journals Preparation of Puerarin Chitosan Oral Nanoparticles by Ionic Gelation Method and Its Related Kinetics

Pharmaceutics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 216 ◽  
Author(s):  
Jie Yan ◽  
Zhi-Yu Guan ◽  
Wei-Feng Zhu ◽  
Ling-Yun Zhong ◽  
Zhuo-Qi Qiu ◽  
...  
Keyword(s):  
In Vitro Release ◽  
Intestinal Perfusion ◽  
Pharmacokinetic Studies ◽  
Ultrasonic Power ◽  
Ionic Gelation ◽  
Single Pass ◽  
Perfusion Model ◽  
Vitro Release

In this paper, as an active ingredient, puerarin chitosan nanoparticles (Pur-CS/TPP-NPs) are prepared by an ionic gelation method. The chitosan (CS) concentration, pH of the CS solution, sodium tripolyphosphate (TPP) concentration, stirring speed, stirring time, ultrasonic power, and dosage are used as single factors for investigation, and the encapsulation efficiency, drug loading capacity, particle size, and polydispersity index (PDI) are used as indicators for investigation. The optimal prescription is determined using the Box–Behnken effect surface design method. The characterization of the best formulation, which is determined via an in vitro release assay and liquid chromatography/tandem mass spectrometry (LC-MS/MS) analysis methods, is used here for pharmacokinetic studies. An in situ single-pass intestinal perfusion model is used to investigate drug absorption in the intestine. After characterization, the morphologies of the nanoparticles are intact. It can be seen from the in vitro release experiments that the equation fitted by the nanoparticles is the Higuchi model, the nanoparticle release process is very stable and without sudden release, indicating that the nanoparticles are well-released in vitro. The pharmacokinetic results and the in situ single-pass intestinal perfusion model study show that the bioavailability and absorption of Pur-CS/TPP-NPs were significantly higher than Pur. Thus, all the results show that the prepared nanoparticles can significantly improve the bioavailability of Pur, and we hope to lay the foundation for the development of new products of Pur.

Intestinal transport of HDND-7, a novel hesperetin derivative, in in vitro MDCK cell and in situ single-pass intestinal perfusion models

Xenobiotica ◽  
2016 ◽  
Vol 47 (8) ◽  
pp. 719-730 ◽  
Author(s):  
Ruonan Chen ◽  
Lan Li ◽  
Chenlin Shen ◽  
Cheng Huang ◽  
Taotao Ma ◽  
...  
Keyword(s):  
Mdck Cell ◽  
Intestinal Transport ◽  
Intestinal Perfusion ◽  
Single Pass

Assessment of Ferrous Glycinate Liposome Absorption Using in Situ Single-Pass Perfusion Model

2017 ◽  
Vol 13 (9) ◽  
Author(s):  
Baomiao Ding ◽  
Xiangzhou Yi ◽  
Li Li ◽  
Hualin Yang
Keyword(s):  
Particle Size ◽  
Phytic Acid ◽  
Intestinal Permeability ◽  
Iron Absorption ◽  
Inhibitory Effects ◽  
Single Pass ◽  
Pass Perfusion ◽  
Absorption Pathways ◽  
Main Factor

AbstractLiposomes could be employed to improve the absorption of iron. The purpose of this study was to estimate the intestinal permeability of ferrous glycinate liposomes and to assess the effects of phytic acid, zinc and particle size on iron absorption usingin situsingle-pass perfusion in rats. The results showed that the absorption of ferrous glycinate liposomes was obviously higher than that of ferrous glycinate. The inhibitory effects of phytic acid and zinc on iron absorption were reduced by incorporating ferrous glycinate into liposomes. The particle size of ferrous glycinate liposomes was also a main factor for affecting iron absorption, and the intestinal permeability of the liposomes decreased with its particle size increasing. The results suggested that liposomes could be a potent delivery system to decrease the inhibitory effects of phytic acid and zinc and to enhance iron absorption. Furthermore, liposomes could alter the absorption pathways of ferrous glycinate.

Prediction of human absorption of a trioxane antimalarial drug (CDRI 99/411) using an in-house validated in situ single-pass intestinal perfusion model

2011 ◽  
Vol 61 (09) ◽  
pp. 532-537 ◽  
Author(s):  
Sheelendra Singh ◽  
Kushalkumar Patel ◽  
Tejaswini Pradhan ◽  
Hefazat Siddiqui ◽  
Shio Singh ◽  
...  
Keyword(s):  
Antimalarial Drug ◽  
Intestinal Perfusion ◽  
Single Pass ◽  
Perfusion Model

scholarly journals Intestinal Absorption Mechanisms of Five Flavonoids from Malus hupehensis (Pamp.) Rehd. Extracts in Situ Single-Pass Intestinal Perfusion and in Vitro Caco-2 Cell Model

2020 ◽  
Author(s):  
Hui Yang ◽  
Zhishu Tang ◽  
Jiangxue Cheng ◽  
Jing Wang ◽  
Junbo Zou ◽  
...  
Keyword(s):  
Cell Model ◽  
Paracellular Pathway ◽  
Intestinal Perfusion ◽  
Cancer Resistance ◽  
Absorption Mechanism ◽  
Single Pass ◽  
Malus Hupehensis ◽  
Intestinal Segments

Abstract Background: Previous studies have shown that Malus hupehensis (Pamp.) Rehd. extracts have anti-oxidant, anti-aging and other effects, its bioavailability is low, however its absorption mechanism is still unclear. To investigate the absorption properties of hyperin, quercitrin, phloridzin, quercetin, and phloretin in total flavonoids of Malus hupehensis (Pamp.) Rehd. Extracts. Methods: In situ single-pass intestinal perfusion model and in vitro Caco-2 cell model were used in this study. The effects of concentration of the extract, administration time, temperature, different intestinal segments, paracellular pathway were analyzed, and the effect of efflux inhibitors, such as the P-gp inhibitor verapamil, the multidrug resistance protein2 (MRP2) inhibitor indomethacin, the breast cancer resistance protein (BCRP) inhibitor reserpine, on the transport were evaluated. As well as EDTA, a tight junction regulator, was studied.Results: The results indicated that the jejunum was the optimal absorption intestine segment of quercitrin, phloridzin, and phloretin. And the greatest absorption intestine segment of quercetin was ileum. Furthermore, it was found that the absorption mechanisms of phloridzin in extract was involved in passive diffusion and the mediation of P-gp and MRP2 should not be neglected. The absorption mechanisms of quercetin and phloretin from extract involved active transport and were accompanied by the participation of efflux transporters, such as P-gp, MRP2 and BCRP. And also the paracellular pathway was involved in hyperin and quercitrin. Conclusion: The absorption mechanisms of five flavonoids from Malus hupehensis (Pamp.) Rehd. extract are related to the concentration of the drugs, intestinal segments, and efflux protein.

The in vitro growth of a three-dimensional human dermal replacement using a single-pass perfusion system

1994 ◽  
Vol 43 (8) ◽  
pp. 740-746 ◽  
Author(s):  
Craig R. Halberstadt ◽  
Robert Hardin ◽  
Kelly Bezverkov ◽  
Dale Snyder ◽  
Lynn Allen ◽  
...  
Keyword(s):  
Three Dimensional ◽  
In Vitro Growth ◽  
Perfusion System ◽  
Single Pass ◽  
Pass Perfusion
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