Preparation of Calcium Phosphate Nanocapsule Including Deoxyribonucleic Acid–Polyethyleneimine–Hyaluronic Acid Ternary Complex for Durable Gene Delivery

2014 ◽  
Vol 103 (1) ◽  
pp. 179-184 ◽  
Author(s):  
Tomoko Ito ◽  
Yoshiyuki Koyama ◽  
Makoto Otsuka
Keyword(s):  
Hyaluronic Acid ◽  
Calcium Phosphate ◽  
Gene Delivery ◽  
Ternary Complex ◽  
Deoxyribonucleic Acid

scholarly journals Enhanced Transfection of Human Mesenchymal Stem Cells Using a Hyaluronic Acid/Calcium Phosphate Hybrid Gene Delivery System

Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 798 ◽  
Author(s):  
Jung Eun Lee ◽  
Yue Yin ◽  
Su Yeon Lim ◽  
E. Seul Kim ◽  
Jaeback Jung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Calcium Phosphate ◽  
Gene Delivery ◽  
Delivery System ◽  
Gene Transfection ◽  
Human Mesenchymal Stem Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Gene Delivery System

Human mesenchymal stem cells (hMSCs) show enormous potential in regenerative medicine and tissue engineering. However, current use of hMSCs in clinics is still limited because there is no appropriate way to control their behavior in vivo, such as differentiation to a desired cell type. Genetic modification may provide an opportunity to control the cells in an active manner. One of the major hurdles for genetic manipulation of hMSCs is the lack of an efficient and safe gene delivery system. Herein, biocompatible calcium phosphate (CaP)-based nanoparticles stabilized with a catechol-derivatized hyaluronic acid (dopa-HA) conjugate were used as a carrier for gene transfection to hMSCs for improved differentiation. Owing to the specific interactions between HA and CD44 of bone marrow-derived hMSCs, dopa-HA/CaP showed significantly higher transfection in hMSCs than branched polyethylenimine (bPEI, MW 25 kDa) with no cytotoxicity. The co-delivery of a plasmid DNA encoding bone morphogenetic protein 2 (BMP-2 pDNA) and micro RNA 148b (miRNA-148b) by dopa-HA/CaP achieved significantly improved osteogenic differentiation of hMSCs.

scholarly journals Stabilization of a hyaluronate-associated gene delivery system using calcium ions

2014 ◽  
Vol 2 (6) ◽  
pp. 936-942 ◽  
Author(s):  
Min Feng ◽  
Basma M. Ibrahim ◽  
Erin M. Wilson ◽  
Kyung-Oh Doh ◽  
Brandon K. Bergman ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Gene Delivery ◽  
Delivery System ◽  
Calcium Ions ◽  
Plasmid Dna ◽  
Ternary Complex ◽  
Gene Delivery System

A “DPH” ternary complex consisting of plasmid DNA (pDNA), intracellularly degradable polyethyleneimine, and hyaluronic acid (HA) was stabilized with Ca2+ ions to prevent aggregation.

scholarly journals The effect of adding hyaluronic acid to calcium phosphate on periapical tissue healing following periradicular surgery in dogs

2014 ◽  
Vol 11 (2) ◽  
pp. 122-129 ◽  
Author(s):  
Wafaa Ahmed Omar Segari ◽  
Dalia Abd El Khalek Radwan ◽  
Mohamed Ayad Abd El Hamid
Keyword(s):  
Hyaluronic Acid ◽  
Calcium Phosphate ◽  
Tissue Healing

Bone regeneration of multichannel biphasic calcium phosphate granules supplemented with hyaluronic acid

2019 ◽  
Vol 99 ◽  
pp. 1058-1066 ◽  
Author(s):  
Mirana Taz ◽  
Preeti Makkar ◽  
Khan Mohammad Imran ◽  
D.W. Jang ◽  
Yong-Sik Kim ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Calcium Phosphate ◽  
Bone Regeneration ◽  
Biphasic Calcium Phosphate
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