scholarly journals In Vitro Protein Binding of Liraglutide in Human Plasma Determined by Reiterated Stepwise Equilibrium Dialysis

2013 ◽  
Vol 102 (8) ◽  
pp. 2882-2888 ◽  
Author(s):  
Anne Plum ◽  
Lisbeth Bjerring Jensen ◽  
Jesper Bøggild Kristensen
Keyword(s):  
Protein Binding ◽  
Human Plasma ◽  
Equilibrium Dialysis ◽  
Vitro Protein

scholarly journals Divergent subcellular locations of HTLV-I tax and Int-6: A contrast between in vitro protein-protein binding and intracellular protein colocalization

1997 ◽  
Vol 4 (5) ◽  
pp. 229-234 ◽  
Author(s):  
Christine Neuveut ◽  
Dong-Yan Jin ◽  
Oliver J. Semmes ◽  
Francesca Diella ◽  
Robert Callahan ◽  
...  
Keyword(s):  
Protein Binding ◽  
Intracellular Protein ◽  
Vitro Protein

scholarly journals The In vitro Effects of Atenolol and Zinc Chloride on the Protein Binding of Amlodipine in Aqueous Medium

1970 ◽  
Vol 7 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Md Shah Amran ◽  
Sheikh Niaz Morshed ◽  
Md Jahangir Alam Khandakar ◽  
Md Masudur Rahman ◽  
Md Mosiur Rahman ◽  
...  
Keyword(s):  
Combination Therapy ◽  
Protein Binding ◽  
Zinc Chloride ◽  
Equilibrium Dialysis ◽  
In Vitro Study ◽  
Pharmacokinetic Properties ◽  
Number Of Binding Sites ◽  
Dialysis Method ◽  
Tissue Systems

An in vitro study of protein binding has been carried out to observe the influence of atenolol and zinc chloride on the protein binding of amlodipine by equilibrium dialysis method at 37±1 0C and at physiological pH (7.4). It has been found that zinc chloride lowered the affinity and percentage of protein binding of amlodipine to bovine serum albumin but atenolol has no such effect. The Scatchard plots were prepared to reveal the number of binding sites and the affinity for protein binding. It was seen that the highest percentage binding of amlodipine was 91% and the lowest was 74%. In the presence of atenolol, the highest and the lowest value of percentage of protein binding was 90% and 72%, respectively. In the presence of zinc chloride these values were 84% and 65% respectively. It is, thus, inferred that atenolol or its complex with amlodipine has no significant effect on percentage of protein binding of amlodipine. While zinc chloride or its complex with amlodipine can cause a decrease in percentage of protein binding of amlodipine. Complexation of amlodipine with zinc chloride might, therefore, displace the drug from the plasma and the displaced drugs may be redistributed, thus , increasing the free drug in plasma and tissue systems. This may change the pharmacokinetic properties of the drug and may affect the pharmacological and toxic effects. It is thus inferred that care and monitoring must be taken during combination therapy of amlodipine and zinc chloride. Key words: Amlodipine, protein binding, binding site, equilibrium dialysis, Scatchard plot, atenolol, zinc chloride, combination therapy  DOI = 10.3329/dujps.v7i1.1202Dhaka Univ. J. Pharm. Sci. 7(1): 15-21, 2008 (June)

scholarly journals In Vitro Bactericidal Activity of Iclaprim in Human Plasma

2009 ◽  
Vol 53 (10) ◽  
pp. 4542-4544 ◽  
Author(s):  
Heike Laue ◽  
Tiziana Valensise ◽  
Aurélie Seguin ◽  
Sergio Lociuro ◽  
Khalid Islam ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Protein Binding ◽  
Human Plasma ◽  
Bactericidal Activity ◽  
The Novel

ABSTRACT This study evaluated the effect of human plasma on the in vitro bactericidal activity of the novel diaminopyrimidine iclaprim against methicillin (meticillin)-susceptible and -resistant Staphylococcus aureus strains. MICs and minimal bactericidal concentrations (MBCs) of iclaprim, with ∼93% protein binding, were similar in the absence and in the presence of 50% human plasma; MICs and MBCs ranged from 0.06 to 0.125 μg/ml. Furthermore, the activity of iclaprim was not affected by plasma, with ≥99.9% reduction in CFU after 5.0 to 7.6 h.

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