Formulation Design and High-Throughput Excipient Selection Based on Structural Integrity and Conformational Stability of Dilute and Highly Concentrated IgG1 Monoclonal Antibody Solutions

2012 ◽  
Vol 101 (3) ◽  
pp. 1120-1135 ◽  
Author(s):  
Akhilesh Bhambhani ◽  
Julian M. Kissmann ◽  
Sangeeta B. Joshi ◽  
David B. Volkin ◽  
Ramesh S. Kashi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
High Throughput ◽  
Structural Integrity ◽  
Conformational Stability ◽  
Formulation Design ◽  
Igg1 Monoclonal Antibody

scholarly journals High-Throughput Biophysical Analysis and Data Visualization of Conformational Stability of an IgG1 Monoclonal Antibody After Deglycosylation

2013 ◽  
Vol 102 (11) ◽  
pp. 3942-3956 ◽  
Author(s):  
Mohammad A. Alsenaidy ◽  
Jae Hyun Kim ◽  
Ranajoy Majumdar ◽  
David D. Weis ◽  
Sangeeta B. Joshi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Data Visualization ◽  
High Throughput ◽  
Conformational Stability ◽  
Igg1 Monoclonal Antibody ◽  
Biophysical Analysis

Effects of Salts from the Hofmeister Series on the Conformational Stability, Aggregation Propensity, and Local Flexibility of an IgG1 Monoclonal Antibody

Biochemistry ◽  
2013 ◽  
Vol 52 (19) ◽  
pp. 3376-3389 ◽  
Author(s):  
Ranajoy Majumdar ◽  
Prakash Manikwar ◽  
John M. Hickey ◽  
Hardeep S. Samra ◽  
Hasige A. Sathish ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Conformational Stability ◽  
Hofmeister Series ◽  
Aggregation Propensity ◽  
Igg1 Monoclonal Antibody ◽  
Local Flexibility

scholarly journals Stress-Based High-Throughput Screening Assays to Identify Inhibitors of Cell Envelope Biogenesis

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 808
Author(s):  
Maurice Steenhuis ◽  
Corinne M. ten Hagen-Jongman ◽  
Peter van Ulsen ◽  
Joen Luirink
Keyword(s):  
Outer Membrane ◽  
High Throughput ◽  
Stress Responses ◽  
High Throughput Screening ◽  
Structural Integrity ◽  
Cell Envelope ◽  
Screening Assay ◽  
High Throughput Screening Assay ◽  
Compound Screening ◽  
Outer Membrane Biogenesis

The structural integrity of the Gram-negative cell envelope is guarded by several stress responses, such as the σE, Cpx and Rcs systems. Here, we report on assays that monitor these responses in E. coli upon addition of antibacterial compounds. Interestingly, compromised peptidoglycan synthesis, outer membrane biogenesis and LPS integrity predominantly activated the Rcs response, which we developed into a robust HTS (high-throughput screening) assay that is suited for phenotypic compound screening. Furthermore, by interrogating all three cell envelope stress reporters, and a reporter for the cytosolic heat-shock response as control, we found that inhibitors of specific envelope targets induce stress reporter profiles that are distinct in quality, amplitude and kinetics. Finally, we show that by using a host strain with a more permeable outer membrane, large-scaffold antibiotics can also be identified by the reporter assays. Together, the data suggest that stress profiling is a useful first filter for HTS aimed at inhibitors of cell envelope processes.

scholarly journals Impact of media and antifoam selection on monoclonal antibody production and quality using a high throughput micro-bioreactor system

2017 ◽  
Vol 34 (1) ◽  
pp. 262-270 ◽  
Author(s):  
Sai Rashmika Velugula-Yellela ◽  
Abasha Williams ◽  
Nicholas Trunfio ◽  
Chih-Jung Hsu ◽  
Brittany Chavez ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
High Throughput ◽  
Antibody Production ◽  
Monoclonal Antibody Production ◽  
Bioreactor System
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