The Study of Amorphous Phase Separation in a Model Polymer Phase-Separating System Using Raman Microscopy and a Low-Temperature Stage: Effect of Cooling Rate and Nucleation Temperature

2011 ◽  
Vol 100 (4) ◽  
pp. 1362-1376 ◽  
Author(s):  
Adora M. Padilla ◽  
Shin G. Chou ◽  
Sumit Luthra ◽  
Michael J. Pikal
Keyword(s):  
Phase Separation ◽  
Low Temperature ◽  
Cooling Rate ◽  
Amorphous Phase ◽  
Raman Microscopy ◽  
Nucleation Temperature ◽  
Polymer Phase

Optimization of a Raman Microscopy Technique to Efficiently Detect Amorphous–Amorphous Phase Separation in Freeze‐Dried Protein Formulations

2014 ◽  
Vol 103 (9) ◽  
pp. 2749-2758 ◽  
Author(s):  
Kelly M. Forney‐Stevens ◽  
Michael J. Pelletier ◽  
Evgenyi Y. Shalaev ◽  
Michael J. Pikal ◽  
Robin H. Bogner
Keyword(s):  
Phase Separation ◽  
Amorphous Phase ◽  
Raman Microscopy ◽  
Microscopy Technique ◽  
Protein Formulations ◽  
Freeze Dried

Bulk standards for low-temperature biological x-ray microanalysis

1984 ◽  
Vol 42 ◽  
pp. 282-283
Author(s):  
P. Echlin ◽  
M. McKoon ◽  
E.S. Taylor ◽  
C.E. Thomas ◽  
K.L. Maloney ◽  
...  
Keyword(s):  
Phase Separation ◽  
Low Temperature ◽  
Analytical Method ◽  
Salt Solutions ◽  
Absolute Concentration ◽  
Cooling Process ◽  
X Ray ◽  
The Absolute ◽  
Analytical Procedures ◽  
Electrical Conductors

Although sections of frozen salt solutions have been used as standards for x-ray microanalysis, such solutions are less useful when analysed in the bulk form. They are poor thermal and electrical conductors and severe phase separation occurs during the cooling process. Following a suggestion by Whitecross et al we have made up a series of salt solutions containing a small amount of graphite to improve the sample conductivity. In addition, we have incorporated a polymer to ensure the formation of microcrystalline ice and a consequent homogenity of salt dispersion within the frozen matrix. The mixtures have been used to standardize the analytical procedures applied to frozen hydrated bulk specimens based on the peak/background analytical method and to measure the absolute concentration of elements in developing roots.

The Application of X-Ray Diffraction at Elevated Temperatures to Study the Mechanism of Formation of Sodium Tripolyphosphate

1961 ◽  
Vol 5 ◽  
pp. 276-284
Author(s):  
E. L. Moore ◽  
J. S. Metcalf
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Amorphous Phase ◽  
Elevated Temperatures ◽  
Sodium Tripolyphosphate ◽  
X Ray Diffraction ◽  
Mechanism Of Formation ◽  
X Ray ◽  
Temperature Form ◽  
High Temperature Form

AbstractHigh-temperature X-ray diffraction techniques were employed to study the condensation reactions which occur when sodium orthophosphates are heated to 380°C. Crystalline Na4P2O7 and an amorphous phase were formed first from an equimolar mixture of Na2HPO4·NaH2PO4 and Na2HPO4 at temperatures above 150°C. Further heating resulted in the formation of Na5P3O10-I (high-temperature form) at the expense of the crystalline Na4P4O7 and amorphous phase. Crystalline Na5P3O10-II (low-temperature form) appears after Na5P3O10-I.Conditions which affect the yield of crystalline Na4P2O7 and amorphous phase as intermediates and their effect on the yield of Na5P3O10 are also presented.

scholarly journals Observation of liquid-liquid phase separation of ataxin-3 and quantitative evaluation of its concentration in a single droplet using Raman microscopy

2021 ◽  
Author(s):  
Kazuki Murakami ◽  
Shinji Kajimoto ◽  
Daiki Shibata ◽  
Kunisato Kuroi ◽  
Fumihiko Fujii ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Neurodegenerative Diseases ◽  
Protein Aggregation ◽  
Quantitative Evaluation ◽  
Raman Microscopy ◽  
Liquid Phase Separation ◽  
Biological Processes ◽  
Single Droplet ◽  
Liquid Liquid Phase Separation

Liquid–liquid phase separation (LLPS) plays an important role in a variety of biological processes and is also associated with protein aggregation in neurodegenerative diseases. Quantification of LLPS is necessary to...

Microstructures in Rapidly Solidified Ll2-TYPE Ni-Al-Cr Alloys

1984 ◽  
Vol 39 ◽  
Author(s):  
S. C. Huang ◽  
K. M. Chang ◽  
E. L. Hall ◽  
R. F. Laforce
Keyword(s):  
Phase Separation ◽  
Cooling Rate ◽  
Phase Reaction ◽  
Microstructural Study ◽  
Melt Spun ◽  
Rapidly Solidified

ABSTRACTA microstructural study of the melt spun alloys Ni75 Al25. Ni72.5 Al22.5Cr5. and Ni67.5A120Cr12.5 was carried out. Variations in grain morpfology; phase separation and ordering structure were observed. The results are discussed in terms of alloy stoichiometry, Cr effect on phase reaction, microsegregation of Al and the cooling rate.

scholarly journals Influence of Bitumen Type and Asphalt Mixture Composition on Low-Temperature Strength Properties According to Various Test Methods

Materials ◽  
2018 ◽  
Vol 11 (11) ◽  
pp. 2118 ◽  
Author(s):  
Marek Pszczola ◽  
Cezary Szydlowski
Keyword(s):  
Tensile Strength ◽  
Low Temperature ◽  
Cooling Rate ◽  
Strength Properties ◽  
Test Methods ◽  
Asphalt Mixtures ◽  
Bending Test ◽  
Mixture Composition ◽  
Tension Stress ◽  
Strength Reserve

In regions with low-temperatures, action transverse cracks can appear in asphalt pavements as a result of thermal stresses that exceed the fracture strength of materials used in asphalt layers. To better understand thermal cracking phenomenon, strength properties of different asphalt mixtures were investigated. Four test methods were used to assess the influence of bitumen type and mixture composition on tensile strength properties of asphalt mixtures: tensile strength was measured using the thermal stress restrained specimen test (TSRST) and the uniaxial tension stress test (UTST), flexural strength was measured using the bending beam test (BBT), and fracture toughness was measured using the semi-circular bending test (SCB). The strength reserve behavior of tested asphalt mixtures was assessed as well. The influence of cooling rate on the strength reserve was investigated and correlations between results from different test methods were also analyzed and discussed. It was observed that the type of bitumen was a factor of crucial importance to low-temperature properties of the tested asphalt concretes. This conclusion was valid for all test methods that were used. It was also observed that the level of cooling rate influenced the strength reserve and, in consequence, resistance to low-temperature cracking. It was concluded that reasonably good correlations were observed between strength results for the UTST, BBT, and SCB test methods.

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