In-line and real-time process monitoring of a freeze drying process using Raman and NIR spectroscopy as complementary process analytical technology (PAT) tools

2009 ◽  
Vol 98 (9) ◽  
pp. 3430-3446 ◽  
Author(s):  
T.R.M. De Beer ◽  
P. Vercruysse ◽  
A. Burggraeve ◽  
T. Quinten ◽  
J. Ouyang ◽  
...  
Keyword(s):  
Real Time ◽  
Process Monitoring ◽  
Freeze Drying ◽  
Process Analytical Technology ◽  
Nir Spectroscopy ◽  
Time Process ◽  
Drying Process ◽  
Analytical Technology

scholarly journals Application of Multivariate Image Analysis to Thermal Images for in line Monitoring of the Freeze-Drying Process

2018 ◽  
Author(s):  
Domenico Colucci ◽  
José Manuel Prats-Montalban ◽  
Davide Fissore ◽  
Alberto Ferrer Riquelme
Keyword(s):  
Image Analysis ◽  
Process Monitoring ◽  
Freeze Drying ◽  
Reference Model ◽  
Process Analytical Technology ◽  
Infrared Camera ◽  
Drying Process ◽  
Thermal Images ◽  
Multivariate Image Analysis ◽  
Multivariate Image

A new Process Analytical Technology (PAT) has been developed and tested for on-line process monitoring of a vacuum freeze-drying process. The sensor uses an infrared camera to obtain thermal images of the ongoing process and multivariate image analysis (MIA) to extract the information after automatic detection and segmentation of the region corresponding to the product in every vial. A reference model was built, using the information of six batches, and different kind of anomalous events, involving either single vials in the batch or the whole batch, were simulated to test the capacity of the MIA-based monitoring system to promptly identify them. Keywords: freeze-drying; process monitoring; infrared image; multivariate image analysis. 

Real-Time Process Analytical Technology Assurance for Flow Synthesis of Oligonucleotides

2014 ◽  
Vol 19 (1) ◽  
pp. 203-214 ◽  
Author(s):  
James W. Rydzak ◽  
David E. White ◽  
Christian Y. Airiau ◽  
Jeffrey T. Sterbenz ◽  
Brian D. York ◽  
...  
Keyword(s):  
Real Time ◽  
Process Analytical Technology ◽  
Time Process ◽  
Flow Synthesis ◽  
Analytical Technology

On-line UV-NIR spectroscopy as a process analytical technology (PAT) tool for on-line and real-time monitoring of the extraction process of Coptis Rhizome

RSC Advances ◽  
2016 ◽  
Vol 6 (12) ◽  
pp. 10078-10085 ◽  
Author(s):  
Xuezhi Dai ◽  
Hang Song ◽  
Wen Liu ◽  
Shun Yao ◽  
Gang Wang
Keyword(s):  
Real Time ◽  
Process Analytical Technology ◽  
Nir Spectroscopy ◽  
Extraction Process ◽  
Real Time Monitoring ◽  
On Line ◽  
Analytical Technology

UV-NIR spectroscopy connected method as a tool for on-line and real-time monitoring of Coptis Rhizome extraction process.

Video real-time imaging of artificial membranes during freeze-drying by cryo-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 16-17
Author(s):  
Alan S. Rudolph ◽  
Ronald R. Price
Keyword(s):  
Real Time ◽  
Self Assembly ◽  
Freeze Drying ◽  
Video Capture ◽  
Drying Process ◽  
Artificial Membranes ◽  
The Past ◽  
Cryo Electron Microscopy ◽  
Spherical Structures ◽  
Capture Techniques

We have employed cryoelectron microscopy to visualize events that occur during the freeze-drying of artificial membranes by employing real time video capture techniques. Artificial membranes or liposomes which are spherical structures within internal aqueous space are stabilized by water which provides the driving force for spontaneous self-assembly of these structures. Previous assays of damage to these structures which are induced by freeze drying reveal that the two principal deleterious events that occur are 1) fusion of liposomes and 2) leakage of contents trapped within the liposome [1]. In the past the only way to access these events was to examine the liposomes following the dehydration event. This technique allows the event to be monitored in real time as the liposomes destabilize and as water is sublimed at cryo temperatures in the vacuum of the microscope. The method by which liposomes are compromised by freeze-drying are largely unknown. This technique has shown that cryo-protectants such as glycerol and carbohydrates are able to maintain liposomal structure throughout the drying process.

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