scholarly journals Suppression of Notch Signaling in Osteoclasts Improves Bone Regeneration and Healing

2019 ◽  
Vol 37 (10) ◽  
pp. 2089-2103 ◽  
Author(s):  
Peeyush N. Goel ◽  
Yasaman Moharrer ◽  
John H. Hebb ◽  
Alexander J. Egol ◽  
Gurpreet Kaur ◽  
...  
Keyword(s):  
Notch Signaling ◽  
Bone Regeneration

scholarly journals Notch-Wnt signal crosstalk regulates proliferation and differentiation of osteoprogenitor cells during intramembranous bone healing

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
S. Lee ◽  
L. H. Remark ◽  
A. M. Josephson ◽  
K. Leclerc ◽  
E. Muiños Lopez ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
Notch Signaling ◽  
Bone Regeneration ◽  
Bone Healing ◽  
Canonical Wnt Signaling ◽  
Osteoprogenitor Cells ◽  
Intramembranous Bone ◽  
Proliferation And Differentiation ◽  
Canonical Wnt ◽  
Wnt Signal

AbstractAdult bone regeneration is orchestrated by the precise actions of osteoprogenitor cells (OPCs). However, the mechanisms by which OPC proliferation and differentiation are linked and thereby regulated are yet to be defined. Here, we present evidence that during intramembranous bone formation OPC proliferation is controlled by Notch signaling, while differentiation is initiated by activation of canonical Wnt signaling. The temporospatial separation of Notch and Wnt signal activation during the early stages of bone regeneration suggests crosstalk between the two pathways. In vitro and in vivo manipulation of the two essential pathways demonstrate that Wnt activation leads to initiation of osteogenic differentiation and at the same time inhibits Notch signaling, which results in termination of the proliferative phase. Here, we establish canonical Wnt signaling as a key regulator that facilitates the crosstalk between OPC proliferation and differentiation during intramembranous, primary bone healing.

scholarly journals JAGGED1 stimulates cranial neural crest cell osteoblast commitment pathways and bone regeneration independent of canonical NOTCH signaling

Bone ◽  
2021 ◽  
Vol 143 ◽  
pp. 115657
Author(s):  
Archana Kamalakar ◽  
Jay M. McKinney ◽  
Daniel Salinas Duron ◽  
Angelica M. Amanso ◽  
Samir A. Ballestas ◽  
...  
Keyword(s):  
Neural Crest ◽  
Notch Signaling ◽  
Bone Regeneration ◽  
Neural Crest Cell ◽  
Cranial Neural Crest ◽  
Cranial Neural Crest Cell ◽  
Crest Cell

Biomaterial-Mediated Presentation of Jagged-1 Mimetic Ligand Enhances Cellular Activation of Notch Signaling and Bone Regeneration

ACS Nano ◽  
2021 ◽  
Author(s):  
Yingrui Deng ◽  
Rui Li ◽  
Haixing Wang ◽  
Boguang Yang ◽  
Peng Shi ◽  
...  
Keyword(s):  
Notch Signaling ◽  
Bone Regeneration ◽  
Cellular Activation

scholarly journals Silibinin Promotes Bone Regeneration Through HIF-1α/VEGF and Notch Signaling Pathway in Ovariectomized Rats

2021 ◽  
Author(s):  
Zhoushan Tao ◽  
Tian-Lin Li ◽  
Min Yang ◽  
Hong-Guang Xu
Keyword(s):  
Notch Signaling ◽  
Bone Regeneration ◽  
Signaling Pathway ◽  
Notch Signaling Pathway ◽  
Systemic Administration ◽  
Ovariectomized Rats ◽  
Micro Ct ◽  
Notch 1 ◽  
Osteogenic Activity ◽  
Qpcr Analysis

Abstract Objective The purpose was to observe whether systemic administration with silibinin(SIL) have an positive effect on bone defect regeneration through HIF-1α/VEGF and Notch signaling pathway in an ovariectomized(OVX) rat model. Methods The MC3T3-E1 cells were co-cultured with lower SIL and higher SIL and induced to osteogenesis, and the cell viability, osteogenic activity were observed by Cell Count Kit-8(CCK-8), Alkaline phosphatase (ALP) staining, Alizarin Red(RES) staining and Western blotting(WB). After the drilling defect model was established, the OVX rats were treated with SIL for 12 weeks. Micro-CT, histology and Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis were used to observe the therapeutic effect and explore the possible mechanism. Results CCK-8, ALP and ARS staining results show that the cell mineralization and osteogenic activity of LSIL and HSIL group is significantly higher than the Con group. Protein expressions show that related regulatory proteins such as ALP, OPN, RUNX-2, OC, VEGFA, HIF-1α, Notch 1, JAG 1, HEY 1 and HES 1 of LSIL and HSIL group are significantly higher than Con group. Micro-CT and Histological analysis evaluation show that group SIL + OVX presented the stronger effect on bone regeneration, bone mineralization, higher expression of VEGFA and HIF-1α, when compared with OVX group. RT-qPCR analysis shows that SIL + OVX group showed increased Notch 1, HES1, HEY1 and JAG1 than the OVX group(p < 0.05). Conclusions Our current study demonstrated that systemic administration with SIL is a scheme for rapid repair of femoral condylar defects, and these effects may be achieved by activating HIF-1α/VEGF and Notch signaling pathway.

Notch Signaling in Bone Regeneration

2011 ◽  
Author(s):  
Samir Mehta ◽  
Kurt Hankenson
Keyword(s):  
Notch Signaling ◽  
Bone Regeneration

scholarly journals Notch signaling components are upregulated during both endochondral and intramembranous bone regeneration

2011 ◽  
Vol 30 (2) ◽  
pp. 296-303 ◽  
Author(s):  
Michael I. Dishowitz ◽  
Shawn P. Terkhorn ◽  
Sandra A. Bostic ◽  
Kurt D. Hankenson
Keyword(s):  
Notch Signaling ◽  
Bone Regeneration ◽  
Intramembranous Bone ◽  
Signaling Components

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

Placenta mesenchymal stem cells on a chorion scaffold as a potential osteogenic graft for peripartal bone regeneration

2008 ◽  
Vol 68 (S 01) ◽  
Author(s):  
S Mohr ◽  
BC Portmann-Lanz ◽  
A Schoeberlein ◽  
R Sager ◽  
DV Surbek
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration

The Role of Activated NOTCH Signaling in Childhood T-ALL

2010 ◽  
Vol 223 (01) ◽  
Author(s):  
AE Kulozik ◽  
C Kox ◽  
M Remke ◽  
S Breit ◽  
M Zimmermann ◽  
...  
Keyword(s):  
Notch Signaling
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