scholarly journals Effect of highly purified capsaicin on articular cartilage and rotator cuff tendon healing: An in vivo rabbit study

2015 ◽  
Vol 33 (12) ◽  
pp. 1854-1860
Author(s):  
Nicole A. Friel ◽  
Allison G. McNickle ◽  
Michael J. DeFranco ◽  
FanChia Wang ◽  
Elizabeth F. Shewman ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Rotator Cuff ◽  
Tendon Healing ◽  
Rotator Cuff Tendon

Decellularized Human Umbilical Cord Wharton Jelly Scaffold Improves Tendon Regeneration in a Rabbit Rotator Cuff Tendon Defect Model

2021 ◽  
pp. 036354652110557
Author(s):  
Zhiguo Yuan ◽  
Fuyang Cao ◽  
Cangjian Gao ◽  
Zhen Yang ◽  
Quanyi Guo ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Umbilical Cord ◽  
Tendon Healing ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Rotator Cuff Tendon ◽  
Tendon Regeneration ◽  
Tendon Defect

Background: Owing to limited self-healing capacity, failure of rotator cuff tendon healing is a common complication after surgery. Biological scaffolds have garnered attention owing to their potential to enhance healing outcomes. Purpose: To verify the effect of the decellularized umbilical cord Wharton jelly (DUCWJ) scaffold as a bridging scaffold in a rabbit model of acute rotator cuff tendon defect. Study Design: Controlled laboratory study. Methods: We fabricated a DUCWJ scaffold using a physicochemical decellularized method, evaluating changes in the umbilical cord Wharton jelly before and after decellularization. Scanning electron microscopy and biomechanical testing were performed to determine the microstructure and mechanical properties. We assessed cytocompatibility and cell regulatory behavior of the scaffold toward tendon stem/progenitor cells (TSPCs). A supraspinatus tendon defect was created in 54 New Zealand White rabbits, allocated to the DUCWJ scaffold repair group and the negative control group (without scaffold). Histology, reverse transcription polymerase chain reaction, and biomechanical tensile strength were assessed at 4, 8, and 12 weeks postoperatively. Results: Decellularization completely removed cells from the umbilical cord Wharton jelly, retained a considerable amount of glycosaminoglycan and collagen, and preserved the microstructure and tensile strength. The DUCWJ scaffold facilitated migration and proliferation of TSPCs in vitro. Tendon-related gene expression revealed that the DUCWJ scaffold could maintain the tenocyte phenotype of TSPCs. In the in vivo study, the DUCWJ scaffold improved tendon healing and enhanced the biomechanical strength of repaired tendons. Histological evaluation scores of the DUCWJ group were significantly higher than those of the negative control at 4, 8, and 12 weeks after surgery ( P < .05). In repaired tendon tissues, reverse transcription polymerase chain reaction findings revealed that the DUCWJ scaffold stimulated tendon development and maturation. Furthermore, an overall increase in ultimate load and tensile modulus was noted over time; the DUCWJ group presented better results than the negative control group ( P < .05). Conclusion: The DUCWJ scaffold has an excellent 3-dimensional porous structure, good biocompatibility, and fundamental biomechanical characteristics, and it promotes migration, attachment, and proliferation of TSPCs. The in vivo animal study demonstrated that the DUCWJ scaffold has potential for tendon regeneration in an acute rotator cuff tendon defect model Clinical Relevance: DUCWJ scaffolds have potential as a regenerative material to augment rotator cuff healing in the clinical setting.

Bioactive LbL-assembled multilayer nanofilms upregulate tenogenesis and angiogenesis enabling robust healing of degenerative rotator cuff tendons in vivo

2019 ◽  
Vol 7 (10) ◽  
pp. 4388-4398 ◽  
Author(s):  
Fei Han ◽  
Peng Zhang ◽  
Xuejun Wen ◽  
Chao Lin ◽  
Peng Zhao
Keyword(s):  
Rotator Cuff ◽  
Multilayer Film ◽  
Tendon Healing

The aim of this work was to fabricate LbL-assembled multilayer film-modified aligned scaffolds and investigate their regulation of TSPCs and potential for degenerative tendon healing.

scholarly journals Therapeutic potential of exosomes in rotator cuff tendon healing

2019 ◽  
Vol 37 (5) ◽  
pp. 759-767 ◽  
Author(s):  
Denton E. Connor ◽  
Jordan A. Paulus ◽  
Parinaz Jila Dabestani ◽  
Finosh K. Thankam ◽  
Matthew F. Dilisio ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Therapeutic Potential ◽  
Tendon Healing ◽  
Rotator Cuff Tendon

scholarly journals In vitro effects of alendronate on fibroblasts of the human rotator cuff tendon

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Chang-Meen Sung ◽  
Ra Jeong Kim ◽  
Young-Sool Hah ◽  
Ji-Yong Gwark ◽  
Hyung Bin Park
Keyword(s):  
Wound Healing ◽  
Rotator Cuff ◽  
Cell Viability ◽  
Caspase 3 ◽  
Tendon Healing ◽  
Cellular Migration ◽  
Mineral Density ◽  
Rotator Cuff Tendon ◽  
Human Rotator Cuff

Abstract Background Bone mineral density of the humeral head is an independent determining factor for postoperative rotator cuff tendon healing. Bisphosphonates, which are commonly used to treat osteoporosis, have raised concerns regarding their relationships to osteonecrosis of the jaw and to atypical fracture of the femur. In view of the prevalence of rotator cuff tear in osteoporotic elderly people, it is important to determine whether bisphosphonates affect rotator cuff tendon healing. However, no studies have investigated bisphosphonates’ cytotoxicity to human rotator cuff tendon fibroblasts (HRFs) or bisphosphonates’ effects on rotator cuff tendon healing. The purpose of this study was to evaluate the cytotoxicity of alendronate (Ald), a bisphosphonate, and its effects on HRF wound healing. Methods HRFs were obtained from human supraspinatus tendons, using primary cell cultures. The experimental groups were control, 0.1 μM Ald, 1 μM Ald, 10 μM Ald, and 100 μM Ald. Alendronate exposure was for 48 h, except during a cell viability analysis with durations from 1 day to 6 days. The experimental groups were evaluated for cell viability, cell cycle and cell proliferation, type of cell death, caspase activity, and wound-healing ability. Results The following findings regarding the 100 μM Ald group contrasted with those for all the other experimental groups: a significantly lower rate of live cells (p < 0.01), a higher rate of subG1 population, a lower rate of Ki-67 positive cells, higher rates of apoptosis and necrosis, a higher number of cells with DNA fragmentation, higher caspase-3/7 activity (p < 0.001), and a higher number of caspase-3 positive staining cells. In scratch-wound healing analyses of all the experimental groups, all the wounds healed within 48 h, except in the 100 μM Ald group (p < 0.001). Conclusions Low concentrations of alendronate appear to have little effect on HRF viability, proliferation, migration, and wound healing. However, high concentrations are significantly cytotoxic, impairing cellular proliferation, cellular migration, and wound healing in vitro.

Tissue engineering and rotator cuff tendon healing

2007 ◽  
Vol 16 (5) ◽  
pp. S204-S207 ◽  
Author(s):  
Joshua S. Dines ◽  
Daniel A. Grande ◽  
David M. Dines
Keyword(s):  
Tissue Engineering ◽  
Rotator Cuff ◽  
Tendon Healing ◽  
Rotator Cuff Tendon

scholarly journals The Effect of Purified Multi-potent Human Bone-marrow Derived Mesenchymal Stem Cells on Rotator Cuff Tendon Healing in an Athymic Rat

2016 ◽  
Vol 4 (7_suppl4) ◽  
pp. 2325967116S0014
Author(s):  
Ryan Degen ◽  
Andrew Carbone ◽  
Camilia Carballo ◽  
Jianchun Zong ◽  
Tony Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Rotator Cuff ◽  
Tendon Healing ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Rotator Cuff Tendon

Biologic Augmentation of Rotator Cuff Tendon-Healing with Use of a Mixture of Osteoinductive Growth Factors*

2007 ◽  
Vol 89 (11) ◽  
pp. 2485-2497 ◽  
Author(s):  
Scott A. Rodeo ◽  
Hollis G. Potter ◽  
Sumito Kawamura ◽  
A. Simon Turner ◽  
Hyon Jeong Kim ◽  
...  
Keyword(s):  
Growth Factors ◽  
Rotator Cuff ◽  
Tendon Healing ◽  
Rotator Cuff Tendon

Biologic Augmentation of Rotator Cuff Tendon-Healing with Use of a Mixture of Osteoinductive Growth Factors*

2007 ◽  
Vol 89 (11) ◽  
pp. 2485-2497 ◽  
Author(s):  
Scott A. Rodeo ◽  
Hollis G. Potter ◽  
Sumito Kawamura ◽  
A. Simon Turner ◽  
Hyon Jeong Kim ◽  
...  
Keyword(s):  
Growth Factors ◽  
Rotator Cuff ◽  
Tendon Healing ◽  
Rotator Cuff Tendon
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