scholarly journals Mechanical stretch enhances COL2A1 expression on chromatin by inducing SOX9 nuclear translocalization in inner meniscus cells

2011 ◽  
Vol 30 (3) ◽  
pp. 468-474 ◽  
Author(s):  
Tomoko Kanazawa ◽  
Takayuki Furumatsu ◽  
Motomi Hachioji ◽  
Toshitaka Oohashi ◽  
Yoshifumi Ninomiya ◽  
...  
Keyword(s):  
Mechanical Stretch ◽  
Col2a1 Expression ◽  
Meniscus Cells

scholarly journals ROCK inhibition stimulates SOX9/Smad3-dependent COL2A1 expression in inner meniscus cells

2016 ◽  
Vol 21 (4) ◽  
pp. 524-529 ◽  
Author(s):  
Takayuki Furumatsu ◽  
Ami Maehara ◽  
Toshifumi Ozaki
Keyword(s):  
Col2a1 Expression ◽  
Meniscus Cells ◽  
Rock Inhibition

scholarly journals Mechanical stretch increases Smad3-dependent CCN2 expression in inner meniscus cells

2012 ◽  
Vol 30 (11) ◽  
pp. 1738-1745 ◽  
Author(s):  
Takayuki Furumatsu ◽  
Tomoko Kanazawa ◽  
Yoshiaki Miyake ◽  
Satoshi Kubota ◽  
Masaharu Takigawa ◽  
...  
Keyword(s):  
Mechanical Stretch ◽  
Meniscus Cells ◽  
Ccn2 Expression

scholarly journals Cyclic Mechanical Stretch Ameliorates the Degeneration of Nucleus Pulposus Cells through Promoting the ITGA2/PI3K/AKT Signaling Pathway

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Dandan Wang ◽  
Yuanzhen Chen ◽  
Shengnan Cao ◽  
Pengcheng Ren ◽  
Haojun Shi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Tensile Stress ◽  
Differentially Expressed Genes ◽  
Mechanical Stretch ◽  
Akt Signaling ◽  
Akt Pathway ◽  
Control Group ◽  
Akt Signaling Pathway ◽  
Stress Group ◽  
Col2a1 Expression

Background. Intervertebral disc degeneration (IVDD) is one of the major causes of low back pain and motor deficiency. Nucleus pulposus (NP) degeneration plays a key role in the process of IVDD. The mechanical and biological interactions involved in NP degeneration have not been elucidated. The present study is aimed at investigating the effect and mechanism of cyclic mechanical stretch in regulating the function and degeneration of NP cells. Methods. NP cells were subjected to cyclic tensile stress (10% deformation) of 0.1 Hz for 8640 cycles. Cell proliferation was conducted through the MTT assay. The cell cycle and apoptosis were detected by flow cytometry. A gene expression profile chip was used to analyze the differentially expressed genes between the tensile stress group and the control group. Enrichment analysis of Gene Ontology (GO) annotation and signaling pathways were analyzed. Western blot and RNA interference were carried out to investigate the role of the ITGA2/PI3K/AKT pathway in the effect of cyclic mechanical stretch on NP cells. Results. NP cells exhibited a greater ( P < 0.05 ) growth rate in the tensile stress group compared to the control group. Cyclic mechanical stress significantly promoted the cell cycle transition of NP cells from the S phase to the G2/M phase. A fewer proportion of apoptotic cells were found in the tensile stress group ( P < 0.05 ), indicating that cyclic mechanical stretch inhibits NP cell apoptosis. Microarray analysis revealed 689 significant differentially expressed genes between the two groups ( P < 0.05 ), of which 333 genes were upregulated and another 356 genes were downregulated. Cyclic mechanical stretch altered the expression of 31 genes involved in the ITGA2/PI3K/AKT pathway and remarkably promoted this pathway in NP cells. Downregulation of ITGA2 and AKT further demonstrated that the PI3K/AKT pathway was responsible for the proliferation and COL2A1 expression of NP cells upon cyclic mechanical stretch. Conclusions. Cyclic mechanical stretch promoted the proliferation and cell cycle and reversely inhibited the apoptosis of NP cells. Cyclic mechanical stretch promoted COL2A1 expression and ameliorated the degeneration of NP cells via regulation of the ITGA2/PI3K/AKT signaling pathway. Our results may provide a potential target and a possibility of IVDD disease treatment by ameliorating the degenerative changes.

How do cardiomyocytes sense stretch? Effects of cyclic mechanical stretch on cardiomyocyte cellular organization

2014 ◽  
Vol 62 (S 01) ◽  
Author(s):  
S. Dhein ◽  
A. Schreiber ◽  
S. Steinbach ◽  
M. Kostelka ◽  
F.W. Mohr
Keyword(s):  
Mechanical Stretch ◽  
Cellular Organization

Mechanical stretch upregulates connexin43 in human trabecular meshwork cells

2019 ◽  
Author(s):  
Nikoleta Tellios ◽  
Mary Feng ◽  
Nancy Chen ◽  
Hong Liu ◽  
Vasiliki Tellios ◽  
...  
Keyword(s):  
Trabecular Meshwork ◽  
Mechanical Stretch ◽  
Trabecular Meshwork Cells

Influence of the mechanical stretch property of fabrics on the wear comfort of men’s suit pants

2021 ◽  
pp. 004051752110191
Author(s):  
Hiroyuki Kanai ◽  
Kentaro Ogawa ◽  
Tetsu Sasagawa ◽  
Kiyohiro Shibata
Keyword(s):  
Mechanical Properties ◽  
Pressure Measurement ◽  
Mechanical Stretch ◽  
The Body ◽  
Weft Yarn ◽  
Elongation Ratio ◽  
Weft Direction ◽  
Wear Comfort ◽  
Finishing Process ◽  
Elastic Thread

The stretch property of fabrics is one of the most important factors that provide comfort to wearers. It is expected that tension building up in the fabric can be relaxed and the garment pressure on the body can be reduced by appropriately exploiting its stretch property. Currently, the stretch property is predominantly realized using spandex. However, weaving or knitting elastic threads cannot be employed for the worsted fabric used to design men’s suits because of their effects on the mechanical properties of the fabric (e.g., embrittlement), which deteriorate with time. In this study, worsted fabric with a graded mechanical stretch property was produced, and the effect of the mechanical stretch property on comfort was verified. The mechanical stretch property is developed from the tension relaxation and fabric shrinkage along the weft yarn during the crabbing, scouring, and drying stages of the finishing process. Then, the form of the fabric is set by heating. In this study, the worsted fabric had an elongation ratio varying from 5.9% to 16.1% along the weft direction that was produced without using elastic thread. Furthermore, men’s suit pants were made from the fabrics. The effect of the stretch property on the garment comfort was verified through sensory evaluation and garment pressure measurement. The contribution of the mechanical stretch property in improving the garment comfort of men’s suit pants is discussed.

Mechanical stretch induces osteogenesis through the alternative activation of macrophages

2021 ◽  
Author(s):  
Lili Dong ◽  
Yang Song ◽  
Yajie Zhang ◽  
Weikang Zhao ◽  
Chunli Wang ◽  
...  
Keyword(s):  
Mechanical Stretch ◽  
Alternative Activation
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