scholarly journals Effects of tensile strain and fluid flow on osteoarthritic human chondrocyte metabolism in vitro

2010 ◽  
Vol 28 (7) ◽  
pp. 907-913 ◽  
Author(s):  
Taro Mawatari ◽  
Derek P. Lindsey ◽  
Alex H.S. Harris ◽  
Stuart B. Goodman ◽  
William J. Maloney ◽  
...  
Keyword(s):  
Fluid Flow ◽  
Tensile Strain ◽  
Human Chondrocyte ◽  
Chondrocyte Metabolism

Effects of intermittent hydrostatic pressure and BMP-2 on osteoarthritic human chondrocyte metabolism in vitro

2010 ◽  
Vol 29 (3) ◽  
pp. 361-368 ◽  
Author(s):  
R.L. Smith ◽  
D.P. Lindsey ◽  
L. Dhulipala ◽  
A.H.S. Harris ◽  
S.B. Goodman ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
Human Chondrocyte ◽  
Chondrocyte Metabolism

scholarly journals O23: CHARACTERISING PATIENT-DERIVED COLORECTAL CANCER TISSUE-ORIGINATED ORGANOIDAL SPHEROIDS FOR HIGH-THROUGHPUT MICROFLUIDIC APPLICATIONS

2021 ◽  
Vol 108 (Supplement_1) ◽  
Author(s):  
MI Khot ◽  
M Levenstein ◽  
R Coppo ◽  
J Kondo ◽  
M Inoue ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fluid Flow ◽  
High Throughput ◽  
Microfluidic Devices ◽  
In Vitro Models ◽  
Fluorescent Imaging ◽  
Cancer Tissue ◽  
Cell Models

Abstract Introduction Three-dimensional (3D) cell models have gained reputation as better representations of in vivo cancers as compared to monolayered cultures. Recently, patient tumour tissue-derived organoids have advanced the scope of complex in vitro models, by allowing patient-specific tumour cultures to be generated for developing new medicines and patient-tailored treatments. Integrating 3D cell and organoid culturing into microfluidics, can streamline traditional protocols and allow complex and precise high-throughput experiments to be performed with ease. Method Patient-derived colorectal cancer tissue-originated organoidal spheroids (CTOS) cultures were acquired from Kyoto University, Japan. CTOS were cultured in Matrigel and stem-cell media. CTOS were treated with 5-fluorouracil and cytotoxicity evaluated via fluorescent imaging and ATP assay. CTOS were embedded, sectioned and subjected to H&E staining and immunofluorescence for ABCG2 and Ki67 proteins. HT29 colorectal cancer spheroids were produced on microfluidic devices using cell suspensions and subjected to 5-fluorouracil treatment via fluid flow. Cytotoxicity was evaluated through fluorescent imaging and LDH assay. Result 5-fluorouracil dose-dependent reduction in cell viability was observed in CTOS cultures (p<0.01). Colorectal CTOS cultures retained the histology, tissue architecture and protein expression of the colonic epithelial structure. Uniform 3D HT29 spheroids were generated in the microfluidic devices. 5-fluorouracil treatment of spheroids and cytotoxic analysis was achieved conveniently through fluid flow. Conclusion Patient-derived CTOS are better complex models of in vivo cancers than 3D cell models and can improve the clinical translation of novel treatments. Microfluidics can streamline high-throughput screening and reduce the practical difficulties of conventional organoid and 3D cell culturing. Take-home message Organoids are the most advanced in vitro models of clinical cancers. Microfluidics can streamline and improve traditional laboratory experiments.

ChemInform Abstract: Synthesis and in vitro Evaluation of 5-Arylidene-3-hydroxyalkyl-2-phenylimino-4-thiazolidinones (V) with Antidegenerative Activity on Human Chondrocyte Cultures.

ChemInform ◽  
2008 ◽  
Vol 39 (11) ◽  
Author(s):  
Rosaria Ottana ◽  
Rosanna Maccari ◽  
Rosella Ciurleo ◽  
Maria Gabriella Vigorita ◽  
Anna Maria Panico ◽  
...  
Keyword(s):  
In Vitro Evaluation ◽  
Human Chondrocyte ◽  
Chondrocyte Cultures

In Vitro Stain Transport in Canaliculi of Rat Femora Under Cyclic Loading

2000 ◽  
Author(s):  
Bixia Li ◽  
Timothy L. Norman
Keyword(s):  
Fluid Flow ◽  
Cyclic Loading ◽  
Confocal Microscopy ◽  
Mechanical Loading ◽  
Cyclic Load ◽  
Flow Behavior ◽  
Load Level ◽  
Static Conditions ◽  
Rat Bone

Abstract In this study, rat femurs were used to test the diffusion and mechanical transport properties of a fluroscein stain tracer in microvessels of bone. Fluroscein was used as a tracer to visualize the fluid flow behavior using confocal microscopy. It was found that stain transport occurs due to diffusion under static conditions and due to mechanical loading. The transport increased with cyclic load level and frequency. Our results also show that stain transport at the canaliculi level occurs rapidly in rat bone.

Observations on fluid flow from exposed dentine in primary teeth: An in vitro study

2017 ◽  
Vol 83 ◽  
pp. 312-316 ◽  
Author(s):  
Monticha Rangcharoen ◽  
Varisara Sirimaharaj ◽  
Sitthichai Wanachantararak ◽  
Noppakun Vongsavan ◽  
Bruce Matthews
Keyword(s):  
Fluid Flow ◽  
Primary Teeth ◽  
In Vitro Study ◽  
Vitro Study

Nanog maintains human chondrocyte phenotype and function in vitro

2009 ◽  
Vol 28 (4) ◽  
pp. 516-521 ◽  
Author(s):  
Hongjun Zheng ◽  
Francoise Gourronc ◽  
Joseph A. Buckwalter ◽  
James A. Martin
Keyword(s):  
Chondrocyte Phenotype ◽  
Human Chondrocyte ◽  
And Function

scholarly journals A Human Chondrocyte-Derived In Vitro Model of Alcohol-Induced and Steroid-Induced Femoral Head Necrosis

2018 ◽  
Vol 24 ◽  
pp. 539-547 ◽  
Author(s):  
Xiong Qin ◽  
Pan Jin ◽  
Tongmeng Jiang ◽  
Muyan Li ◽  
Jiachang Tan ◽  
...  
Keyword(s):  
Femoral Head ◽  
In Vitro Model ◽  
Femoral Head Necrosis ◽  
Human Chondrocyte ◽  
Vitro Model

In Vitro Experimental Investigation of the Integrity of the Stem–Cement Interface

2013 ◽  
Vol 647 ◽  
pp. 53-56
Author(s):  
Hong Yu Zhang ◽  
Leigh Fleming ◽  
Liam Blunt
Keyword(s):  
Experimental Investigation ◽  
Fluid Flow ◽  
Hip Replacement ◽  
Hip Prosthesis ◽  
Vitro Experiment ◽  
Cement Interface ◽  
In Vitro Experiment ◽  
Mechanical Bonding

The rationale behind failure of cemented total hip replacement is still far from being well understood in a mechanical and molecular perspective. In the present study, the integrity of the stem–cement interface was investigated through an in vitro experiment monitoring fluid flow along this interface. The results indicated that a good mechanical bonding formed at the stem–cement interface before debonding of this interface was induced by physiological loadings during the in vivo service of the hip prosthesis.

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