scholarly journals Improved bioengineered cartilage tissue formation following cyclic compression is dependent on upregulation of MT1-MMP

2010 ◽  
Vol 28 (7) ◽  
pp. 921-927 ◽  
Author(s):  
J. N. Amrith De Croos ◽  
Peter J. Roughley ◽  
Rita A. Kandel
Keyword(s):  
Cartilage Tissue ◽  
Tissue Formation ◽  
Cyclic Compression

Calcium regulates cyclic compression-induced early changes in chondrocytes during in vitro cartilage tissue formation

Cell Calcium ◽  
2010 ◽  
Vol 48 (4) ◽  
pp. 232-242 ◽  
Author(s):  
Igal Raizman ◽  
J.N. Amritha De Croos ◽  
Robert Pilliar ◽  
Rita A. Kandel
Keyword(s):  
Cartilage Tissue ◽  
Tissue Formation ◽  
Cyclic Compression

scholarly journals Cartilage tissue formation from human adipose-derived stem cells via herbal component (Avocado/soybean unsaponifiables) in scaffold-free culture system

2020 ◽  
Vol 17 (1) ◽  
pp. 54
Author(s):  
Nazem Ghasemi ◽  
Arefeh Basiri ◽  
Batool Hashemibeni ◽  
Mohammad Kazemi ◽  
Ali Valiani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Culture System ◽  
Cartilage Tissue ◽  
Adipose Derived Stem Cells ◽  
Tissue Formation

Cartilage tissue formation through assembly of microgels containing mesenchymal stem cells

2018 ◽  
Vol 77 ◽  
pp. 48-62 ◽  
Author(s):  
Fanyi Li ◽  
Vinh X. Truong ◽  
Philipp Fisch ◽  
Clara Levinson ◽  
Veronica Glattauer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cartilage Tissue ◽  
Tissue Formation

Primary chondrocytes enhance cartilage tissue formation upon co-culture with a range of cell types

Soft Matter ◽  
2010 ◽  
Vol 6 (20) ◽  
pp. 5080 ◽  
Author(s):  
Jeanine A. A. Hendriks ◽  
Razvan L. Miclea ◽  
Roka Schotel ◽  
Ewart de Bruijn ◽  
Lorenzo Moroni ◽  
...  
Keyword(s):  
Cell Types ◽  
Cartilage Tissue ◽  
Tissue Formation ◽  
Primary Chondrocytes

scholarly journals In Vitro Fabrication of Hybrid Bone/Cartilage Complex Using Mouse Induced Pluripotent Stem Cells

2020 ◽  
Vol 21 (2) ◽  
pp. 581 ◽  
Author(s):  
Phoonsuk Limraksasin ◽  
Takeru Kondo ◽  
Maolin Zhang ◽  
Hiroko Okawa ◽  
Thanaphum Osathanon ◽  
...  
Keyword(s):  
Cartilage Tissue ◽  
Marker Genes ◽  
Induction Medium ◽  
Mechanical Stimuli ◽  
Tissue Formation ◽  
Osteogenic Induction Medium ◽  
Osteogenic Induction ◽  
Chondrogenic Marker ◽  
Induced Pluripotent

Cell condensation and mechanical stimuli play roles in osteogenesis and chondrogenesis; thus, they are promising for facilitating self-organizing bone/cartilage tissue formation in vitro from induced pluripotent stem cells (iPSCs). Here, single mouse iPSCs were first seeded in micro-space culture plates to form 3-dimensional spheres. At day 12, iPSC spheres were subjected to shaking culture and maintained in osteogenic induction medium for 31 days (Os induction). In another condition, the osteogenic induction medium was replaced by chondrogenic induction medium at day 22 and maintained for a further 21 days (Os-Chon induction). Os induction produced robust mineralization and some cartilage-like tissue, which promoted expression of osteogenic and chondrogenic marker genes. In contrast, Os-Chon induction resulted in partial mineralization and a large area of cartilage tissue, with greatly increased expression of chondrogenic marker genes along with osterix and collagen 1a1. Os-Chon induction enhanced mesodermal lineage commitment with brachyury expression followed by high expression of lateral plate and paraxial mesoderm marker genes. These results suggest that combined use of micro-space culture and mechanical stimuli facilitates hybrid bone/cartilage tissue formation from iPSCs, and that the bone/cartilage tissue ratio in iPSC constructs could be manipulated through the induction protocol.

Cartilage Tissue Formation Using Redifferentiated Passaged ChondrocytesIn Vitro

2009 ◽  
Vol 15 (3) ◽  
pp. 665-673 ◽  
Author(s):  
Nazish Ahmed ◽  
Lu Gan ◽  
Andras Nagy ◽  
Jianing Zheng ◽  
Chen Wang ◽  
...  
Keyword(s):  
Cartilage Tissue ◽  
Tissue Formation

scholarly journals Dual Delivery of TGF-β3 and Ghrelin in Microsphere/Hydrogel Systems for Cartilage Regeneration

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5732
Author(s):  
Jianjing Lin ◽  
Li Wang ◽  
Jianhao Lin ◽  
Qiang Liu
Keyword(s):  
Growth Factors ◽  
Chondrogenic Differentiation ◽  
Transforming Growth Factor ◽  
Double Emulsion ◽  
Cartilage Regeneration ◽  
Cartilage Tissue ◽  
Self Healing ◽  
Tissue Formation ◽  
Extraction Technology

Articular cartilage (AC) damage is quite common, but due to AC’s poor self-healing ability, the damage can easily develop into osteoarthritis (OA). To solve this problem, we developed a microsphere/hydrogel system that provides two growth factors that promote cartilage repair: transforming growth factor-β3 (TGF-β3) to enhance cartilage tissue formation and ghrelin synergy TGF-β to significantly enhance the chondrogenic differentiation. The hydrogel and microspheres were characterized in vitro, and the biocompatibility of the system was verified. Double emulsion solvent extraction technology (w/o/w) is used to encapsulate TGF-β3 and ghrelin into microspheres, and these microspheres are encapsulated in a hydrogel to continuously release TGF-β3 and ghrelin. According to the chondrogenic differentiation ability of mesenchymal stem cells (MSCs) in vitro, the concentrations of the two growth factors were optimized to promote cartilage regeneration.

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