Hypoxia upregulates the expression of angiopoietin-like-4 in human articular chondrocytes: Role of angiopoietin-like-4 in the expression of matrix metalloproteinases and cartilage degradation

2009 ◽  
Vol 27 (1) ◽  
pp. 50-57 ◽  
Author(s):  
Minako Murata ◽  
Kazuo Yudo ◽  
Hiroshi Nakamura ◽  
Junji Chiba ◽  
Kazuki Okamoto ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Articular Chondrocytes ◽  
Cartilage Degradation ◽  
Human Articular Chondrocytes ◽  
And Cartilage

scholarly journals The Lysine Specific Demethylase-1 Negatively Regulates the COL9A1 Gene in Human Articular Chondrocytes

2020 ◽  
Vol 21 (17) ◽  
pp. 6322
Author(s):  
Anne-Laure Durand ◽  
Alexandre Dufour ◽  
Elisabeth Aubert-Foucher ◽  
Christine Oger-Desfeux ◽  
Marielle Pasdeloup ◽  
...  
Keyword(s):  
Articular Chondrocytes ◽  
Cartilage Matrix ◽  
Human Articular Chondrocytes ◽  
Collagen Molecule ◽  
Sequencing Analysis ◽  
Loss Of Function ◽  
Gene Encoding ◽  
Lysine Specific Demethylase ◽  
Function Strategy

Osteoarthritis (OA) is a degenerative disease of the joints which is associated with an impaired production of the cartilage matrix by the chondrocytes. Here, we investigated the role of Lysine-Specific Demethylase-1 (LSD1), a chromatin remodeling enzyme whose role in articular chondrocytes was previously associated with a catabolic activity and which is potentially involved during OA. Following a loss of function strategy and RNA sequencing analysis, we detail the genes which are targeted by LSD1 in human articular chondrocytes and identify COL9A1, a gene encoding the α1 chain of the cartilage-specific type IX collagen, as negatively regulated by LSD1. We show that LSD1 interacts with the transcription factor SOX9 and is recruited to the promoter of COL9A1. Interestingly, we observe that OA cartilage displays stronger LSD1 immunostaining compared with normal, and we demonstrate that the depletion of LSD1 in OA chondrocytes prevents the decrease in COL9A1 following Il-1β treatment. These results suggest LSD1 is a new regulator of the anabolic activity of articular chondrocytes potentially destabilizing the cartilage matrix, since it negatively regulates COL9A1, a gene encoding a crucial anchoring collagen molecule. This newly identified role played by LSD1 may thus participate in the alteration of the cartilage matrix during OA.

scholarly journals Role of Hyaluronan in Inflammatory Effects on Human Articular Chondrocytes

Inflammation ◽  
2019 ◽  
Vol 42 (5) ◽  
pp. 1808-1820 ◽  
Author(s):  
Mary K. Cowman ◽  
Claire Shortt ◽  
Shivani Arora ◽  
Yuhong Fu ◽  
Jemma Villavieja ◽  
...  
Keyword(s):  
Articular Chondrocytes ◽  
Human Articular Chondrocytes

scholarly journals The role of norepinephrine in human articular chondrocytes

2012 ◽  
Vol 20 ◽  
pp. S144-S145
Author(s):  
J. Kaps ◽  
R.H. Straub ◽  
M. Böhm ◽  
J. Beckmann ◽  
J. Grifka ◽  
...  
Keyword(s):  
Articular Chondrocytes ◽  
Human Articular Chondrocytes

META060 inhibits osteoclastogenesis and matrix metalloproteinases in vitro and reduces bone and cartilage degradation in a mouse model of rheumatoid arthritis

2010 ◽  
Vol 62 (6) ◽  
pp. 1683-1692 ◽  
Author(s):  
Veera Reddy Konda ◽  
Anuradha Desai ◽  
Gary Darland ◽  
Jeffrey S. Bland ◽  
Matthew L. Tripp
Keyword(s):  
Rheumatoid Arthritis ◽  
Matrix Metalloproteinases ◽  
Mouse Model ◽  
Cartilage Degradation ◽  
And Cartilage
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