scholarly journals The effect of oxygen tension on the in vitro assay of human osteoblastic connective tissue progenitor cells

2008 ◽  
Vol 26 (10) ◽  
pp. 1390-1397 ◽  
Author(s):  
Sandra M. Villarruel ◽  
Cynthia A. Boehm ◽  
Mark Pennington ◽  
Jason A. Bryan ◽  
Kimerly A. Powell ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Progenitor Cells ◽  
Oxygen Tension ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Effect Of Oxygen ◽  
Connective Tissue Progenitor Cells

scholarly journals Feeder-free and serum-free in vitro assay for measuring the effect of drugs on acute and chronic myeloid leukemia stem/progenitor cells

2020 ◽  
Vol 90 ◽  
pp. 52-64.e11 ◽  
Author(s):  
Hanyang Lin ◽  
Jackie E. Damen ◽  
Marta A. Walasek ◽  
Stephen J. Szilvassy ◽  
Ali G. Turhan ◽  
...  
Keyword(s):  
Chronic Myeloid Leukemia ◽  
Progenitor Cells ◽  
Myeloid Leukemia ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Serum Free

scholarly journals Release from Quiescence of Primitive Human Hematopoietic Stem/Progenitor Cells by Blocking Their Cell-Surface TGF-β Type II Receptor in a Short-Term In Vitro Assay

Stem Cells ◽  
2000 ◽  
Vol 18 (2) ◽  
pp. 102-111 ◽  
Author(s):  
Nicolas Fortunel ◽  
Jacques Hatzfeld ◽  
Sergueï Kisselev ◽  
Marie-Noëlle Monier ◽  
Karin Ducos ◽  
...  
Keyword(s):  
Cell Surface ◽  
Progenitor Cells ◽  
In Vitro Assay ◽  
Type Ii ◽  
Short Term ◽  
Vitro Assay ◽  
Hematopoietic Stem

scholarly journals Analysis of Patient Factors Affecting In Vitro Characteristics of Subacromial Bursal Connective Tissue Progenitor Cells during Rotator Cuff Repair

2021 ◽  
Vol 10 (17) ◽  
pp. 4006
Author(s):  
Daichi Morikawa ◽  
Benjamin C. Hawthorne ◽  
Mary Beth R. McCarthy ◽  
Nicholas Bellas ◽  
Jeremiah D. Johnson ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Connective Tissue ◽  
Progenitor Cells ◽  
Rank Correlation ◽  
Supraspinatus Tendon ◽  
Patient Demographics ◽  
Spearman’S Rank Correlation ◽  
Tear Size ◽  
Connective Tissue Progenitor Cells

Unsatisfactory failure rates following rotator cuff (RC) repair have led orthopaedic surgeons to explore biological augmentation of the healing enthesis. The subacromial bursa (SB) contains abundant connective tissue progenitor cells (CTPs) that may aid in this process. The purpose of the study was to investigate the influence of patient demographics and tear characteristics on the number of colony-forming units (CFUs) and nucleated cell count (NCC) of SB-derived CTPs. In this study, we harvested SB tissue over the supraspinatus tendon and muscle in 19 patients during arthroscopic RC repair. NCC of each sample was analyzed on the day of the procedure. After 14 days, CFUs were evaluated under a microscope. Spearman’s rank correlation coefficient was then used to determine the relationship between CFUs or NCC and patient demographics or tear characteristics. The study found no significant correlation between patient demographics and the number of CFUs or NCC of CTPs derived from the SB (p > 0.05). The study did significantly observe that increased tear size was negatively correlated with the number of CFUs (p < 0.05). These results indicated that increased tear size, but not patient demographics, may influence the viability of CTPs and should be considered when augmenting RCrepairs with SB.

scholarly journals Isolation and characterization of connective tissue progenitor cells derived from human fracture-induced hemarthrosis in vitro

2008 ◽  
Vol 26 (2) ◽  
pp. 190-199 ◽  
Author(s):  
Sang Yang Lee ◽  
Masahiko Miwa ◽  
Yoshitada Sakai ◽  
Ryosuke Kuroda ◽  
Keisuke Oe ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Progenitor Cells ◽  
Isolation And Characterization ◽  
Connective Tissue Progenitor Cells

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

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