scholarly journals Isolation and characterization of connective tissue progenitor cells derived from human fracture-induced hemarthrosis in vitro

2008 ◽  
Vol 26 (2) ◽  
pp. 190-199 ◽  
Author(s):  
Sang Yang Lee ◽  
Masahiko Miwa ◽  
Yoshitada Sakai ◽  
Ryosuke Kuroda ◽  
Keisuke Oe ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Progenitor Cells ◽  
Isolation And Characterization ◽  
Connective Tissue Progenitor Cells

scholarly journals The effect of oxygen tension on the in vitro assay of human osteoblastic connective tissue progenitor cells

2008 ◽  
Vol 26 (10) ◽  
pp. 1390-1397 ◽  
Author(s):  
Sandra M. Villarruel ◽  
Cynthia A. Boehm ◽  
Mark Pennington ◽  
Jason A. Bryan ◽  
Kimerly A. Powell ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Progenitor Cells ◽  
Oxygen Tension ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Effect Of Oxygen ◽  
Connective Tissue Progenitor Cells

scholarly journals Isolation and Characterization of Fetal Leydig Progenitor Cells of Male Mice

Endocrinology ◽  
2015 ◽  
Vol 157 (3) ◽  
pp. 1222-1233 ◽  
Author(s):  
Miki Inoue ◽  
Yuichi Shima ◽  
Kanako Miyabayashi ◽  
Kaori Tokunaga ◽  
Tetsuya Sato ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Leydig Cells ◽  
Fluorescent Protein ◽  
Growth Factor Receptor ◽  
Citrate Cycle ◽  
Isolation And Characterization ◽  
Expression Of Genes ◽  
Green Fluorescent

Abstract Fetal and adult Leydig cells develop in mammalian prenatal and postnatal testes, respectively. In mice, fetal Leydig cells (FLCs) emerge in the interstitial space of the testis at embryonic day 12.5 and thereafter increase in number, possibly through differentiation from progenitor cells. However, the progenitor cells have not yet been identified. Previously, we established transgenic mice in which FLCs are labeled strongly with enhanced green fluorescent protein (EGFP). Interestingly, fluorescence-activated cell sorting provided us with weakly EGFP-labeled cells as well as strongly EGFP-labeled FLCs. In vitro reconstruction of fetal testes demonstrated that weakly EGFP-labeled cells contain FLC progenitors. Transcriptome from the 2 cell populations revealed, as expected, marked differences in the expression of genes required for growth factor/receptor signaling and steroidogenesis. In addition, genes for energy metabolisms such as glycolytic pathways and the citrate cycle were activated in strongly EGFP-labeled cells, suggesting that metabolism is activated during FLC differentiation.

scholarly journals Analysis of Patient Factors Affecting In Vitro Characteristics of Subacromial Bursal Connective Tissue Progenitor Cells during Rotator Cuff Repair

2021 ◽  
Vol 10 (17) ◽  
pp. 4006
Author(s):  
Daichi Morikawa ◽  
Benjamin C. Hawthorne ◽  
Mary Beth R. McCarthy ◽  
Nicholas Bellas ◽  
Jeremiah D. Johnson ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Connective Tissue ◽  
Progenitor Cells ◽  
Rank Correlation ◽  
Supraspinatus Tendon ◽  
Patient Demographics ◽  
Spearman’S Rank Correlation ◽  
Tear Size ◽  
Connective Tissue Progenitor Cells

Unsatisfactory failure rates following rotator cuff (RC) repair have led orthopaedic surgeons to explore biological augmentation of the healing enthesis. The subacromial bursa (SB) contains abundant connective tissue progenitor cells (CTPs) that may aid in this process. The purpose of the study was to investigate the influence of patient demographics and tear characteristics on the number of colony-forming units (CFUs) and nucleated cell count (NCC) of SB-derived CTPs. In this study, we harvested SB tissue over the supraspinatus tendon and muscle in 19 patients during arthroscopic RC repair. NCC of each sample was analyzed on the day of the procedure. After 14 days, CFUs were evaluated under a microscope. Spearman’s rank correlation coefficient was then used to determine the relationship between CFUs or NCC and patient demographics or tear characteristics. The study found no significant correlation between patient demographics and the number of CFUs or NCC of CTPs derived from the SB (p > 0.05). The study did significantly observe that increased tear size was negatively correlated with the number of CFUs (p < 0.05). These results indicated that increased tear size, but not patient demographics, may influence the viability of CTPs and should be considered when augmenting RCrepairs with SB.

PENGEMBANGAN TEKNOLOGI SELEKSI IN VITRO BERULANG (REPEAT CYCLING–IN VITRO SELECTION) TOLERAN STRES KEKERINGAN UNTUK PERBAIKAN MUTU GENETIK DAN PRODUKSI TANAMAN KEDELAI BERMIKORIZA

2017 ◽  
Vol 1 (1) ◽  
pp. 74-84
Author(s):  
Ahmad Riduan ◽  
Rainiyati Rainiyati ◽  
Yulia Alia
Keyword(s):  
Arbuscular Mycorrhizae ◽  
In Vitro Selection ◽  
Soil Samples ◽  
Single Spore ◽  
Isolation And Characterization ◽  
Single Spore Culture ◽  
Spore Culture ◽  
Glomus Sp

Every plant rhizospheres in any ecosystem there are various living microorganisms including Arbuscular Mycorrhizae Fungi (AMF).  An isolation and characterization is required to investigate the species or type of the AMF. This research was aimed at studying the isolation and characterization of AMF sporulation in soybean rhizospheres in Jambi Province. The results of evaluation on soil samples before trapping showed that there are spores from three genus of AMF twelve types Glomus , two types Acaulospora and one type of Enthrophospora.  Following single spore culture in soybean rhizosphere, 5 spore types were obtained:  Glomus sp-1, Glomus sp-4, Glomus sp-7, Glomus sp-8 Glomus sp-10.

scholarly journals Isolation and characterization of bone marrow-derived mesenchymal progenitor cells with myogenic and neuronal properties

2007 ◽  
Vol 313 (5) ◽  
pp. 1008-1023 ◽  
Author(s):  
Mitsutaka Shiota ◽  
Toshio Heike ◽  
Munetada Haruyama ◽  
Shiro Baba ◽  
Atsunori Tsuchiya ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Mesenchymal Progenitor Cells ◽  
Isolation And Characterization

Characterization of Apoptosis Signaling Cascades During the Differentiation Process of Human Neural ReNcell VM Progenitor Cells In Vitro

2015 ◽  
Vol 35 (8) ◽  
pp. 1203-1216 ◽  
Author(s):  
Alexandra Jaeger ◽  
Michael Fröhlich ◽  
Susanne Klum ◽  
Margareta Lantow ◽  
Torsten Viergutz ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Signaling Cascades ◽  
Differentiation Process

Arginine synthesis by hepatomas in vitro. II. Isolation and characterization of Morris hepatoma variants unable to convert ornithine to arginine, and modulation of urea-cycle enzymes by dexamethasone and cyclic-AMP

1984 ◽  
Vol 68 (1) ◽  
pp. 305-319
Author(s):  
S.J. Goss
Keyword(s):  
Cyclic Amp ◽  
Liver Cells ◽  
Isolation And Characterization ◽  
Rat Liver Cells ◽  
Morris Hepatoma ◽  
Ornithine Transcarbamoylase ◽  
Cellular Incorporation

‘77orn’, a derivative of the Morris rat hepatoma 7777, stably expresses high levels of ornithine transcarbamoylase (OTC) and carbamoylphosphate synthetase I (CPS-I), and is able to grow indefinitely in ornithine-medium (medium with ornithine in place of arginine). Variants that have lost this ability are isolated from 77orn by a ‘suicide’ selective technique dependent on the cellular incorporation of [3H]ornithine. These variants, which have reduced levels of CPS-I, or of both CPS-I and OTC, are shown to have developed multiple hormonal requirements; their enzyme deficiencies can be reversed by use of an appropriately supplemented medium. In particular, CPS-I is inducible by dexamethasone and dibutyryl-cyclic-AMP in combination. Cholera toxin can be used instead of cyclic-AMP, but then butyrate is additionally required if the induction is to be maintained in the long term. The use of these agents in excess can depress OTC. Several other hepatomas, and alos explanted foetal rat liver cells, have similar requirements for CPS-I expression. It is argued that multiple hormonal requirements for CPS-I production are normal in liver cells in vitro, and that hormone-independent hepatomas should be regarded as abnormal. The implications of this for the somatic cell genetic investigation of differentiation are briefly discussed.

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