scholarly journals Canine ACL fibroblast integrin expression and cell alignment in response to cyclic tensile strain in three-dimensional collagen gels

2006 ◽  
Vol 24 (3) ◽  
pp. 481-490 ◽  
Author(s):  
D. Ross Henshaw ◽  
Erik Attia ◽  
Madhu Bhargava ◽  
Jo A. Hannafin
Keyword(s):  
Tensile Strain ◽  
Three Dimensional ◽  
Integrin Expression ◽  
Cell Alignment ◽  
Collagen Gels ◽  
Cyclic Tensile Strain

scholarly journals Alteration of collagen-dependent adhesion, motility, and morphogenesis by the expression of antisense alpha 2 integrin mRNA in mammary cells

1995 ◽  
Vol 108 (2) ◽  
pp. 595-607 ◽  
Author(s):  
P.J. Keely ◽  
A.M. Fong ◽  
M.M. Zutter ◽  
S.A. Santoro
Keyword(s):  
Cell Surface ◽  
Three Dimensional ◽  
Cellular Growth ◽  
Laminin Receptor ◽  
Breast Carcinoma Cell Line ◽  
Integrin Expression ◽  
Mammary Cells ◽  
Collagen Gels ◽  
T47d Cells ◽  
Beta 1 Integrin

Although integrins are known to mediate adhesive binding of cells to the extracellular matrix, their role in mediating cellular growth, morphology, and differentiation is less clear. To determine more directly the role of the alpha 2 beta 1 integrin, a collagen and laminin receptor, in mediating the collagen-dependent differentiation of mammary cells, we reduced expression of the integrin by the well differentiated human breast carcinoma cell line, T47D, by stably expressing alpha 2 integrin antisense mRNA. Flow cytometry demonstrated that the antisense-expressing clones had levels of alpha 2 beta 1 integrin on their surfaces that were decreased by 30–70%. Adhesion of antisense-expressing clones to both collagens I and IV was decreased relative to controls in a manner that correlated with the level of cell surface alpha 2 beta 1 integrin expression. Adhesion to fibronectin and laminin were not affected. Motility across collagen-coated filters in haptotaxis assays was increased for only those clones that exhibited intermediate levels of adhesion to collagen, suggesting that an intermediate density of cell-surface alpha 2 beta 1 integrin optimally supports cell motility. When cultured in three-dimensional collagen gels, T47D cells organized in a manner suggestive of a glandular epithelium. In contrast, antisense-expressing clones with decreased alpha 2 beta 1 integrin were not able to organize in three-dimensional collagen gels. The growth rate of T47D cells was reduced when the cells were cultured in three-dimensional collagen gels. Unlike adhesion, motility, and morphogenesis, growth rates were unaffected by reduction of alpha 2 beta 1 integrin expression. Our results suggest that adhesive interactions mediated by a critical level of surface alpha 2 beta 1 integrin expression are key determinants of the collagen-dependent morphogenetic capacity of mammary epithelial cells.

scholarly journals Lymphocyte locomotion and attachment on two-dimensional surfaces and in three-dimensional matrices

1982 ◽  
Vol 92 (3) ◽  
pp. 747-752 ◽  
Author(s):  
WS Haston ◽  
JM Shields ◽  
PC Wilkinson
Keyword(s):  
Three Dimensional ◽  
Two Dimensional ◽  
Collagen Gels ◽  
Peripheral Lymph Node ◽  
Cellulose Esters ◽  
Peripheral Lymph ◽  
The Matrix ◽  
Identical Material ◽  
Variable Morphology ◽  
Collagen Lattices

The adhesion and locomotion of mouse peripheral lymph node lymphocytes on 2-D protein- coated substrata and in 3-D matrices were compared. Lymphocytes did not adhere to, or migrate on, 2-D substrata suck as serum- or fibronectin-coated glass. They did attach to and migrate in hydrated 3-D collagen lattices. When the collagen was dehydrated to form a 2-D surface, lymphocyte attachment to it was reduced. We propose that lymphocytes, which are poorly adhesive, are able to attach to and migrate in 3-D matrices by a nonadhesive mechanism such as the extension and expansion of pseudopodia through gaps in the matrix, which could provide purchase for movement in the absence of discrete intermolecular adhesions. This was supported by studies using serum-coated micropore filters, since lymphocytes attached to and migrated into filters with pore sizes large enough (3 or 8 mum) to allow pseudopod penetration but did not attach to filters made of an identical material (cellulose esters) but of narrow pore size (0.22 or 0.45 mum). Cinematographic studies of lymphocyte locomotion in collagen gels were also consistent with the above hypothesis, since lymphocytes showed a more variable morphology than is typically seen on plane surfaces, with formation of many small pseudopodia expanded to give a marked constriction between the cell and the pseudopod. These extensions often remained fixed with respect to the environment as the lymphocyte moved away from or past them. This suggests that the pseudopodia were inserted into gaps in the gel matrix and acted as anchorage points for locomotion.

Strain Engineering of Thermal Conductivity of Two-Dimensional MoS2 and h-BN

MRS Advances ◽  
2016 ◽  
Vol 1 (32) ◽  
pp. 2297-2302 ◽  
Author(s):  
Xiaonan Wang ◽  
Alireza Tabarraei
Keyword(s):  
Thermal Conductivity ◽  
Tensile Strain ◽  
Phonon Dispersion ◽  
Hexagonal Boron Nitride ◽  
Three Dimensional ◽  
Strain Engineering ◽  
Normal Strain ◽  
Dynamics Modeling ◽  
Molecular Dynamics Modeling ◽  
The Impact

ABSTRACTWe have used reverse nonequlibrium molecular dynamics modeling to study the impact of uniaxial stretching on the thermal conductivity of monolayer molybdenum disulfide (MoS2) and hexagonal boron nitride (h-BN). Our results predict an anomalous response of the thermal conductivity of these materials to normal strain. Thermal conductivity of h-BN increases under a tensile strain whereas thermal conductivity of MoS2 remains fairly constant. These are in striking contrast to the impact of tensile strain on the thermal conductivity of three dimensional materials whose thermal conductivity decreases under tensile strain. We investigate the mechanism responsible for this unexpected behavior by studying the impact of tensile strain on the phonon dispersion curves and group velocities of these materials.

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