In vitro growth of bovine articular cartilage chondrocytes in various capacitively coupled electrical fields

1984 ◽  
Vol 2 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Carl T. Brighton ◽  
Anthony S. Unger ◽  
Jeffery L. Stambough
Keyword(s):  
Articular Cartilage ◽  
In Vitro Growth ◽  
Capacitively Coupled ◽  
Electrical Fields ◽  
Bovine Articular Cartilage

Regulation of Articular Cartilage Volumetric and Compressive Properties by Sequential Application of IGF-1 and TGF-β1 During In Vitro Growth

2010 ◽  
Author(s):  
Kristin J. Dills ◽  
Jennifer R. Van Donk ◽  
Gregory M. Williams ◽  
Stephanie A. Smith ◽  
Albert C. Chen ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
In Vitro Growth ◽  
Compressive Properties ◽  
Low Friction ◽  
Volumetric Growth ◽  
Bearing Material ◽  
Mechanical Integrity ◽  
Naturally Occurring ◽  
Tgf Β1

Articular cartilage (AC) is a load bearing material that provides a low friction wear resistant interface in synovial joints. Naturally-occurring and stimulated intrinsic repair of damaged AC is challenging [1]. Thus, there is a need to guide the formation of tissue of specific size and mechanical properties for AC repair. Previous studies [2,3] with immature cartilage have shown that culture with medium containing TGF-β1 results in enhanced mechanical integrity while culture with IGF-1 results in diminished mechanical integrity. Conversely, culture with medium containing IGF-1 results in substantial volumetric growth while culture with TGF-β 1 results in little or no volumetric growth.

scholarly journals 206 THE RESPONSE OF BOVINE ARTICULAR CARTILAGE TO CYCLIC LOADING IN VITRO

2009 ◽  
Vol 17 ◽  
pp. S117-S118
Author(s):  
Z. Robertson ◽  
J.E. Jeffrey ◽  
A.S. Plumb ◽  
R.M. Aspden
Keyword(s):  
Articular Cartilage ◽  
Cyclic Loading ◽  
Bovine Articular Cartilage

scholarly journals Articular cartilage mechanical and biochemical property relations before and after in vitro growth

2007 ◽  
Vol 40 (16) ◽  
pp. 3607-3614 ◽  
Author(s):  
Timothy Ficklin ◽  
Gregory Thomas ◽  
James C. Barthel ◽  
Anna Asanbaeva ◽  
Eugene J. Thonar ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Biochemical Property ◽  
In Vitro Growth ◽  
Property Relations ◽  
Before And After

scholarly journals In vitro Articular Cartilage Growth with Sequential Application of IGF-1 and TGF-β1 Enhances Volumetric Growth and Maintains Compressive Properties

2012 ◽  
Vol 134 (3) ◽  
Author(s):  
Nathan T. Balcom ◽  
Britta Berg-Johansen ◽  
Kristin J. Dills ◽  
Jennifer R. Van Donk ◽  
Gregory M. Williams ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage ◽  
Growth Factor ◽  
Extreme Values ◽  
Volumetric Growth ◽  
Bovine Articular Cartilage ◽  
Poisson's Ratios ◽  
Poisson’S Ratios ◽  
Tgf Β1

In vitro cultures with insulin-like growth factor-1 (IGF-1) and transforming growth factor-β1 (TGF-β1) have previously been shown to differentially modulate the growth of immature bovine articular cartilage. IGF-1 stimulates expansive growth yet decreases compressive moduli and increases compressive Poisson’s ratios, whereas TGF-β1 maintains tissue size, increases compressive moduli, and decreases compressive Poisson’s ratios. The current study’s hypothesis was that sequential application of IGF-1 and TGF-β1 during in vitro culture produces geometric and compressive mechanical properties that lie between extreme values produced when using either growth factor alone. Immature bovine articular cartilage specimens were harvested and either untreated (D0, i.e., day zero) or cultured in vitro for either 6 days with IGF-1 (D6 IGF), 12 days with IGF-1 (D12 IGF), or 6 days with IGF-1 followed by 6 days with TGF-β1 (D12 SEQ, i.e., sequential). Following treatment, all specimens were tested for geometric, biochemical, and compressive mechanical properties. Relative to D0, D12 SEQ treatment enhanced volumetric growth, but to a lower value than that for D12 IGF. Furthermore, D12 SEQ treatment maintained compressive moduli and Poisson’s ratios at values higher and lower, respectively, than those for D12 IGF. Considering the previously described effects of 12 days of treatment with TGF-β1 alone, D12 SEQ induced both growth and mechanical property changes between those produced with either IGF-1 or TGF-β1 alone. The results suggest that it may be possible to vary the durations of select growth factors, including IGF-1 and TGF-β1, to more precisely modulate the geometric, biochemical, and mechanical properties of immature cartilage graft tissue in clinical repair strategies.

The effects of radiofrequency energy probe speed and application force on chondrocyte viability

2007 ◽  
Vol 20 (01) ◽  
pp. 34-37 ◽  
Author(s):  
M. L. Meyer ◽  
J. J Bogdanske ◽  
M. D. Markel ◽  
Y. Lu
Keyword(s):  
Articular Cartilage ◽  
In Vitro Study ◽  
Confocal Laser ◽  
Radiofrequency Energy ◽  
Chondrocyte Viability ◽  
Bovine Articular Cartilage ◽  
Cell Staining ◽  
Chondrocyte Death ◽  
Improper Use

Summary Objective: To determine the thermal effects of monopolar radiofrequency energy (mRFE) on bovine articular cartilage when it was moved at different speeds and using varying application forces. Methods: Thirty-six fresh osteochondral sections divided into two groups (18 sections/group) were used in this study. The first group was tested at three speed rates of mRFE probe (1 mm/sec, 5 mm/sec and 10 mm/sec) at a constant force (50 g) applied to the probe tip. In the second group, three application forces of the probe tip were tested (25 g, 50 g and 75 g) at a constant speed (5 mm/sec) (n=6/test). All tests were performed using a custom-built jig to control the mRFE (Vulcan EAS™) probe during a 20-mm pass on each section. After treatment, viability of osteochondral sections was determined by confocal laser microscopy (CLM) combined with vital cell staining. Results: There were not any significant differences in cartilage thickness of tested osteochondral sections among the different speeds or forces. During the mRFE probe treatments at different speeds, CLM demonstrated that probe application at the speed of 1 mm/ sec caused significantly greater chondrocyte death than at the speeds of 5 and 10 mm/sec, whereas there were no significant differences in chondrocyte death among the variable application forces (p>0.05). Discussion: This in vitro study demonstrated that RFE thermal penetration correlated most closely with probe application speed than application force for this mRFE probe. Clinical relevance: Improper use of mRFE may cause thermal injury on articular cartilage.

Comparison of inhibitory effects of glucosamine and mannosamine on bovine articular cartilage degradation in vitro

2004 ◽  
Vol 65 (10) ◽  
pp. 1440-1445 ◽  
Author(s):  
David M. Mello ◽  
Brian D. Nielsen ◽  
Tonia L. Peters ◽  
John P. Caron ◽  
Michael W. Orth
Keyword(s):  
Articular Cartilage ◽  
Cartilage Degradation ◽  
Inhibitory Effects ◽  
Bovine Articular Cartilage
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