Astrocytes contribute to the neuronal recovery promoted by high‐frequency repetitive magnetic stimulation in in vitro models of ischemia

2021 ◽  
Author(s):  
Cláudio Roque ◽  
Nuno Pinto ◽  
Maria Vaz Patto ◽  
Graça Baltazar
Keyword(s):  
High Frequency ◽  
Magnetic Stimulation ◽  
In Vitro Models ◽  
Repetitive Magnetic Stimulation

scholarly journals Repetitive low intensity magnetic field stimulation in a neuronal cell line: A metabolomics study

2018 ◽  
Author(s):  
Ivan Hong ◽  
Andrew Garrett ◽  
Garth Maker ◽  
Ian Mullaney ◽  
Jennifer Rodger ◽  
...  
Keyword(s):  
Cell Line ◽  
Magnetic Stimulation ◽  
Neuronal Excitability ◽  
Therapeutic Potential ◽  
Neuronal Cell ◽  
Neural Tissue ◽  
Gaba Release ◽  
Low Intensity ◽  
Repetitive Magnetic Stimulation

Low intensity repetitive magnetic stimulation of neural tissue modulates neuronal excitability and has promising therapeutic potential in the treatment of neurological disorders. However, the underpinning cellular and biochemical mechanisms remain poorly understood. This study investigates the behavioural effects of low intensity repetitive magnetic stimulation (LI-rMS) at a cellular and biochemical level. We delivered LI-rMS (10 mT) at 1 Hz and 10 Hz (n=5 wells per group) to B50 rat neuroblastoma cells in vitro for 10 minutes and measured levels of selected metabolites immediately after stimulation. LI-rMS at both frequencies depleted selected tricarboxylic acid (TCA) cycle metabolites without affecting the main energy supplies. Furthermore, LI-rMS effects were frequency-specific with 1 Hz stimulation having stronger effects than 10 Hz. The observed depletion of metabolites was consistent with an increase in GABA release as a result of higher spontaneous activity. Although the absence of organised neural circuits and other cellular contributors (e.g. excitatory neurons and glia) in the B50 cell line limits the degree to which our results can be extrapolated to the human brain, the changes we describe provide novel insights into how LI-rMS modulates neural tissue.

scholarly journals Repetitive low intensity magnetic field stimulation in a neuronal cell line: a metabolomics study

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4501 ◽  
Author(s):  
Ivan Hong ◽  
Andrew Garrett ◽  
Garth Maker ◽  
Ian Mullaney ◽  
Jennifer Rodger ◽  
...  
Keyword(s):  
Cell Line ◽  
Magnetic Stimulation ◽  
Neuronal Excitability ◽  
Therapeutic Potential ◽  
Neuronal Cell ◽  
Neural Tissue ◽  
Gaba Release ◽  
Low Intensity ◽  
Repetitive Magnetic Stimulation

Low intensity repetitive magnetic stimulation of neural tissue modulates neuronal excitability and has promising therapeutic potential in the treatment of neurological disorders. However, the underpinning cellular and biochemical mechanisms remain poorly understood. This study investigates the behavioural effects of low intensity repetitive magnetic stimulation (LI-rMS) at a cellular and biochemical level. We delivered LI-rMS (10 mT) at 1 Hz and 10 Hz to B50 rat neuroblastoma cellsin vitrofor 10 minutes and measured levels of selected metabolites immediately after stimulation. LI-rMS at both frequencies depleted selected tricarboxylic acid (TCA) cycle metabolites without affecting the main energy supplies. Furthermore, LI-rMS effects were frequency-specific with 1 Hz stimulation having stronger effects than 10 Hz. The observed depletion of metabolites suggested that higher spontaneous activity may have led to an increase in GABA release. Although the absence of organised neural circuits and other cellular contributors (e.g., excitatory neurons and glia) in the B50 cell line limits the degree to which our results can be extrapolated to the human brain, the changes we describe provide novel insights into how LI-rMS modulates neural tissue.

Repetitive Transcranial Magnetic Stimulation of the Brain

2016 ◽  
Vol 23 (1) ◽  
pp. 82-94 ◽  
Author(s):  
Alexander Tang ◽  
Gary Thickbroom ◽  
Jennifer Rodger
Keyword(s):  
Transcranial Magnetic Stimulation ◽  
Magnetic Stimulation ◽  
Therapeutic Potential ◽  
Repetitive Transcranial Magnetic Stimulation ◽  
Experimental Studies ◽  
Basic Research ◽  
In Vitro Models ◽  
In Vivo Studies

Since the development of transcranial magnetic stimulation (TMS) in the early 1980s, a range of repetitive TMS (rTMS) protocols are now available to modulate neuronal plasticity in clinical and non-clinical populations. However, despite the wide application of rTMS in humans, the mechanisms underlying rTMS-induced plasticity remain uncertain. Animal and in vitro models provide an adjunct method of investigating potential synaptic and non-synaptic mechanisms of rTMS-induced plasticity. This review summarizes in vitro experimental studies, in vivo studies with intact rodents, and preclinical models of selected neurological disorders—Parkinson’s disease, depression, and stroke. We suggest that these basic research findings can contribute to the understanding of how rTMS-induced plasticity can be modulated, including novel mechanisms such as neuroprotection and neurogenesis that have significant therapeutic potential.

scholarly journals Repetitive low intensity magnetic field stimulation in a neuronal cell line: A metabolomics study

2018 ◽  
Author(s):  
Ivan Hong ◽  
Andrew Garrett ◽  
Garth Maker ◽  
Ian Mullaney ◽  
Jennifer Rodger ◽  
...  
Keyword(s):  
Cell Line ◽  
Magnetic Stimulation ◽  
Neuronal Excitability ◽  
Therapeutic Potential ◽  
Neuronal Cell ◽  
Neural Tissue ◽  
Gaba Release ◽  
Low Intensity ◽  
Repetitive Magnetic Stimulation

Low intensity repetitive magnetic stimulation of neural tissue modulates neuronal excitability and has promising therapeutic potential in the treatment of neurological disorders. However, the underpinning cellular and biochemical mechanisms remain poorly understood. This study investigates the behavioural effects of low intensity repetitive magnetic stimulation (LI-rMS) at a cellular and biochemical level. We delivered LI-rMS (10 mT) at 1 Hz and 10 Hz (n=5 wells per group) to B50 rat neuroblastoma cells in vitro for 10 minutes and measured levels of selected metabolites immediately after stimulation. LI-rMS at both frequencies depleted selected tricarboxylic acid (TCA) cycle metabolites without affecting the main energy supplies. Furthermore, LI-rMS effects were frequency-specific with 1 Hz stimulation having stronger effects than 10 Hz. The observed depletion of metabolites was consistent with an increase in GABA release as a result of higher spontaneous activity. Although the absence of organised neural circuits and other cellular contributors (e.g. excitatory neurons and glia) in the B50 cell line limits the degree to which our results can be extrapolated to the human brain, the changes we describe provide novel insights into how LI-rMS modulates neural tissue.

Sign in / Sign up

Export Citation Format

Share Document