scholarly journals Live cell imaging reveals differential modifications to cytoplasmic dynein properties by phospho- and dephosphomimic mutations of the intermediate chain 2C S84

2014 ◽  
Vol 92 (9) ◽  
pp. 1143-1154 ◽  
Author(s):  
Kiev R. Blasier ◽  
Michael K. Humsi ◽  
Junghoon Ha ◽  
Mitchell W. Ross ◽  
W. Russell Smiley ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Cytoplasmic Dynein ◽  
Live Cell ◽  
Intermediate Chain

Intermediate chain subunit as a probe for cytoplasmic dynein function: Biochemical analyses and live cell imaging in PC12 cells

2007 ◽  
Vol 85 (12) ◽  
pp. 2640-2647 ◽  
Author(s):  
Kenneth R. Myers ◽  
Kevin W.-H. Lo ◽  
R. John Lye ◽  
John M. Kogoy ◽  
Violetta Soura ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Cytoplasmic Dynein ◽  
Live Cell ◽  
Biochemical Analyses ◽  
Intermediate Chain

scholarly journals Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments

2005 ◽  
Vol 168 (5) ◽  
pp. 697-703 ◽  
Author(s):  
Yan He ◽  
Franto Francis ◽  
Kenneth A. Myers ◽  
Wenqian Yu ◽  
Mark M. Black ◽  
...  
Keyword(s):  
Rna Interference ◽  
Axonal Transport ◽  
Heavy Chain ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Sympathetic Neurons ◽  
Cytoplasmic Dynein ◽  
Live Cell ◽  
Cytoskeletal Elements

Recent studies have shown that the transport of microtubules (MTs) and neurofilaments (NFs) within the axon is rapid, infrequent, asynchronous, and bidirectional. Here, we used RNA interference to investigate the role of cytoplasmic dynein in powering these transport events. To reveal transport of MTs and NFs, we expressed EGFP-tagged tubulin or NF proteins in cultured rat sympathetic neurons and performed live-cell imaging of the fluorescent cytoskeletal elements in photobleached regions of the axon. The occurrence of anterograde MT and retrograde NF movements was significantly diminished in neurons that had been depleted of dynein heavy chain, whereas the occurrence of retrograde MT and anterograde NF movements was unaffected. These results support a cargo model for NF transport and a sliding filament model for MT transport.

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