Intermediate chain subunit as a probe for cytoplasmic dynein function: Biochemical analyses and live cell imaging in PC12 cells

2007 ◽  
Vol 85 (12) ◽  
pp. 2640-2647 ◽  
Author(s):  
Kenneth R. Myers ◽  
Kevin W.-H. Lo ◽  
R. John Lye ◽  
John M. Kogoy ◽  
Violetta Soura ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Cytoplasmic Dynein ◽  
Live Cell ◽  
Biochemical Analyses ◽  
Intermediate Chain

scholarly journals Live cell imaging reveals differential modifications to cytoplasmic dynein properties by phospho- and dephosphomimic mutations of the intermediate chain 2C S84

2014 ◽  
Vol 92 (9) ◽  
pp. 1143-1154 ◽  
Author(s):  
Kiev R. Blasier ◽  
Michael K. Humsi ◽  
Junghoon Ha ◽  
Mitchell W. Ross ◽  
W. Russell Smiley ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Cytoplasmic Dynein ◽  
Live Cell ◽  
Intermediate Chain

Role of Doc2α in dense-core vesicle exocytosis in PC12 cells revealed by live cell imaging

2011 ◽  
Vol 71 ◽  
pp. e213
Author(s):  
Takashi Tsuboi ◽  
Yasunori Mori ◽  
Hideki Matsui ◽  
Ryo Aoki ◽  
Manami Oya ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Exocytosis

scholarly journals Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments

2005 ◽  
Vol 168 (5) ◽  
pp. 697-703 ◽  
Author(s):  
Yan He ◽  
Franto Francis ◽  
Kenneth A. Myers ◽  
Wenqian Yu ◽  
Mark M. Black ◽  
...  
Keyword(s):  
Rna Interference ◽  
Axonal Transport ◽  
Heavy Chain ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Sympathetic Neurons ◽  
Cytoplasmic Dynein ◽  
Live Cell ◽  
Cytoskeletal Elements

Recent studies have shown that the transport of microtubules (MTs) and neurofilaments (NFs) within the axon is rapid, infrequent, asynchronous, and bidirectional. Here, we used RNA interference to investigate the role of cytoplasmic dynein in powering these transport events. To reveal transport of MTs and NFs, we expressed EGFP-tagged tubulin or NF proteins in cultured rat sympathetic neurons and performed live-cell imaging of the fluorescent cytoskeletal elements in photobleached regions of the axon. The occurrence of anterograde MT and retrograde NF movements was significantly diminished in neurons that had been depleted of dynein heavy chain, whereas the occurrence of retrograde MT and anterograde NF movements was unaffected. These results support a cargo model for NF transport and a sliding filament model for MT transport.

scholarly journals Label-Free Detection of Neuronal Differentiation in Cell Populations Using High-Throughput Live-Cell Imaging of PC12 Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56690 ◽  
Author(s):  
Sebastian Weber ◽  
María L. Fernández-Cachón ◽  
Juliana M. Nascimento ◽  
Steffen Knauer ◽  
Barbara Offermann ◽  
...  
Keyword(s):  
Neuronal Differentiation ◽  
Pc12 Cells ◽  
High Throughput ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Cell Populations ◽  
Label Free ◽  
Label Free Detection

scholarly journals 2PS044 Live cell imaging of the growth factor-induced nuclear translocation of ERK in PC12 cells(The 50th Annual Meeting of the Biophysical Society of Japan)

2012 ◽  
Vol 52 (supplement) ◽  
pp. S117
Author(s):  
Yuki Shindo ◽  
Kazunari Mouri ◽  
Kayo Hibino ◽  
Masaru Tomita ◽  
Yasushi Sako ◽  
...  
Keyword(s):  
Growth Factor ◽  
Annual Meeting ◽  
Pc12 Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Nuclear Translocation ◽  
Live Cell ◽  
Biophysical Society
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