Proteomic identification of biomarkers in the cerebrospinal fluid in a rat model of nigrostriatal dopaminergic degeneration

2007 ◽  
Vol 85 (16) ◽  
pp. 3607-3618 ◽  
Author(s):  
I. Rite ◽  
S. Argüelles ◽  
J.L. Venero ◽  
S. García-Rodriguez ◽  
A. Ayala ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Rat Model ◽  
Proteomic Identification ◽  
Dopaminergic Degeneration

Activation of P2X3 receptors in the cerebrospinal fluid-contacting nucleus neurons reduces formalin-induced pain behavior via PAG in a rat model

Neuroscience ◽  
2017 ◽  
Vol 358 ◽  
pp. 93-102 ◽  
Author(s):  
Peng-Fei Liu ◽  
Hong-Zhi Fang ◽  
Yan Yang ◽  
Qing-Qing Zhang ◽  
Qiang-Qiang Zhou ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Rat Model ◽  
Pain Behavior ◽  
P2x3 Receptors

scholarly journals Comparative Efficacies of Antibiotics in a Rat Model of Meningoencephalitis Due to Listeria monocytogenes

1999 ◽  
Vol 43 (7) ◽  
pp. 1651-1656 ◽  
Author(s):  
Christian Michelet ◽  
Stephen L. Leib ◽  
Daniele Bentue-Ferrer ◽  
Martin G. Täuber
Keyword(s):  
Cerebrospinal Fluid ◽  
Listeria Monocytogenes ◽  
Rat Model ◽  
Antibacterial Effect ◽  
Antibacterial Activities ◽  
The Other ◽  
Active Therapy ◽  
Treatment Regimens ◽  
Infant Rats ◽  
The Brain

ABSTRACT The antibacterial activities of amoxicillin-gentamicin, trovafloxacin, trimethoprim-sulfamethoxazole (TMP-SMX) and the combination of trovafloxacin with TMP-SMX were compared in a model of meningoencephalitis due to Listeria monocytogenes in infant rats. At 22 h after intracisternal infection, the cerebrospinal fluid was cultured to document meningitis, and the treatment was started. Treatment was instituted for 48 h, and efficacy was evaluated 24 h after administration of the last dose. All tested treatment regimens exhibited significant activities in brain, liver, and blood compared to infected rats receiving saline (P < 0.001). In the brain, amoxicillin plus gentamicin was more active than all of the other regimens, and trovafloxacin was more active than TMP-SMX (bacterial titers of 4.1 ± 0.5 log10 CFU/ml for amoxicillin-gentamicin, 5.0 ± 0.4 log10 CFU/ml for trovafloxacin, and 5.8 ± 0.5 log10 CFU/ml for TMP-SMX;P < 0.05). In liver, amoxicillin-gentamicin and trovafloxacin were similarly active (2.8 ± 0.8 and 2.7 ± 0.8 log10 CFU/ml, respectively) but more active than TMP-SMX (4.4 ± 0.6 log10 CFU/ml; P< 0.05). The combination of trovafloxacin with TMP-SMX did not alter the antibacterial effect in the brain, but it did reduce the effect of trovafloxacin in the liver. Amoxicillin-gentamicin was the most active therapy in this study, but the activity of trovafloxacin suggests that further studies with this drug for the treatment ofListeria infections may be warranted.

scholarly journals P2X4R Overexpression Upregulates Interleukin-6 and Exacerbates 6-OHDA-Induced Dopaminergic Degeneration in a Rat Model of PD

2020 ◽  
Vol 12 ◽  
Author(s):  
Jiangnan Ma ◽  
Jinzhao Gao ◽  
Mengyue Niu ◽  
Xiaona Zhang ◽  
Jing Wang ◽  
...  
Keyword(s):  
Rat Model ◽  
Interleukin 6 ◽  
Dopaminergic Degeneration

scholarly journals Proteomic identification of vanin-1 as a marker of kidney damage in a rat model of type 1 diabetic nephropathy

2011 ◽  
Vol 80 (3) ◽  
pp. 272-281 ◽  
Author(s):  
Tim Fugmann ◽  
Beatrice Borgia ◽  
Csaba Révész ◽  
Mária Godó ◽  
Carol Forsblom ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Rat Model ◽  
Kidney Damage ◽  
Proteomic Identification

scholarly journals Proteomic Identification of Biomarkers in the Cerebrospinal Fluid (CSF) of Astrocytoma Patients

2007 ◽  
Vol 6 (2) ◽  
pp. 559-570 ◽  
Author(s):  
Fatima W. Khwaja ◽  
Matthew S. Reed ◽  
Jeffrey J. Olson ◽  
Brian J. Schmotzer ◽  
G. Yancey Gillespie ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Proteomic Identification

scholarly journals Sporadic amyotrophic lateral sclerosis (SALS) – skeletal muscle response to cerebrospinal fluid from SALS patients in a rat model

2018 ◽  
Vol 11 (4) ◽  
pp. dmm031997 ◽  
Author(s):  
Shruthi Shanmukha ◽  
Gayathri Narayanappa ◽  
Atchayaram Nalini ◽  
Phalguni Anand Alladi ◽  
Trichur R. Raju
Keyword(s):  
Skeletal Muscle ◽  
Amyotrophic Lateral Sclerosis ◽  
Cerebrospinal Fluid ◽  
Rat Model ◽  
Sporadic Amyotrophic Lateral Sclerosis ◽  
Muscle Response ◽  
Lateral Sclerosis

scholarly journals Characterization of an APP/tau rat model of Alzheimer’s disease by positron emission tomography and immunofluorescent labeling

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Thomas Filip ◽  
Severin Mairinger ◽  
Joerg Neddens ◽  
Michael Sauberer ◽  
Stefanie Flunkert ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Cerebrospinal Fluid ◽  
Rat Model ◽  
Brain Regions ◽  
Emission Tomography ◽  
Transgenic Rats ◽  
Positive Correlation ◽  
Cross Breeding ◽  
Regional Brain ◽  
Positron Emission

Abstract Background To better understand the etiology and pathomechanisms of Alzheimer’s disease, several transgenic animal models that overexpress human tau or human amyloid-beta (Aβ) have been developed. In the present study, we generated a novel transgenic rat model by cross-breeding amyloid precursor protein (APP) rats with tau rats. We characterized this model by performing positron emission tomography scans combined with immunofluorescent labeling and cerebrospinal fluid analyses. Methods APP/Tau rats were generated by cross-breeding male McGill-R-Thy1-APP transgenic rats with female hTau-40/P301L transgenic rats. APP/Tau double transgenic rats and non-transgenic (ntg) littermates aged 7, 13, and 21 months were subjected to dynamic [11C] PiB scan and dynamic [18F]THK-5317 scans. For regional brain analysis, a template was generated from anatomical MR images of selected animals, which was co-registered with the PET images. Regional analysis was performed by application of the simplified reference tissue model ([11C]PiB data), whereas [18F]THK-5317 data were analyzed using a 2-tissue compartment model and Logan graphical analysis. In addition, immunofluorescent labeling (tau, amyloid) and cerebrospinal fluid analyses were performed. Results [11C]PiB binding potential (BPND) and [18F]THK-5317 volume of distribution (VT) showed an increase with age in several brain regions in the APP/Tau group but not in the ntg control group. Immunohistochemical analysis of brain slices of PET-scanned animals revealed a positive correlation between Aβ labeling and [11C]PiB regional BPND. Tau staining yielded a trend towards higher levels in the cortex and hippocampus of APP/Tau rats compared with ntg littermates, but without reaching statistical significance. No correlation was found between tau immunofluorescence labeling results and the respective [18F]THK-5317 VT values. Conclusions We thoroughly characterized a novel APP/Tau rat model using combined PET imaging and immunofluorescence analysis. We observed an age-related increase in [11C]PiB and [18F]THK-5317 binding in several brain regions in the APP/Tau group but not in the ntg group. Although we were able to reveal a positive correlation between amyloid labeling and [11C]PiB regional brain uptake, we observed relatively low human tau and amyloid fibril expression levels and a somewhat unstable brain pathology which questions the utility of this animal model for further studies.

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