Treatment of cerebellar granule cell neurons with the neurotrophic factor pigment epithelium-derived factor in vitro enhances expression of other neurotrophic factors as well as cytokines and chemokines

2004 ◽  
Vol 77 (5) ◽  
pp. 642-652 ◽  
Author(s):  
Takeshi Yabe ◽  
J. Taylor Herbert ◽  
Asako Takanohashi ◽  
Joan P. Schwartz
Keyword(s):  
Granule Cell ◽  
Neurotrophic Factors ◽  
Neurotrophic Factor ◽  
Pigment Epithelium ◽  
Cerebellar Granule Cell ◽  
Cerebellar Granule ◽  
Cytokines And Chemokines ◽  
Pigment Epithelium Derived Factor

scholarly journals Neurexins play a crucial role in cerebellar granule cell survival by organizing autocrine machinery for neurotrophins

2020 ◽  
Author(s):  
Takeshi Uemura ◽  
Emi Suzuki ◽  
Shiori Kawase ◽  
Taiga Kurihara ◽  
Misato Yasumura ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Survival ◽  
Granule Cell ◽  
Neurotrophic Factors ◽  
Neurotrophic Factor ◽  
Cell Adhesion Molecules ◽  
Cerebellar Granule Cell ◽  
Cerebellar Granule ◽  
Protein Clusters ◽  
Presynaptic Protein

ABSTRACTNeurexins (NRXNs) are among the key presynaptic cell adhesion molecules that regulate synapse function and formation via trans-synaptic interaction with postsynaptic ligands. Here, we generated cerebellar granule cell (CGC)-specific Nrxn triple-knockout (TKO) mice to allow the deletion of all NRXNs. Unexpectedly, most CGCs died in these mice. The requirement of NRNXs for cell survival was reproduced in cultured CGCs. We showed that the axons of cultured Nrxn TKO CGCs that were not in contact with the postsynaptic structure had defects in the formation of presynaptic protein cluster and action potential-induced Ca2+ influx. Additionally, these cells were impaired in the secretion from axons of depolarization-induced fluorescence-tagged brain-derived neurotrophic factor (BDNF), and the cell-survival defect was rescued by the application of BDNF. Our results suggest that CGC survival is maintained by autocrine neurotrophic factors, and that NRXNs organize the presynaptic protein clusters and the autocrine neurotrophic factor secretory machinery independent of contact with postsynaptic ligands.

Experimental modification of postnatal cerebellar granule cell migration in vitro

1986 ◽  
Vol 377 (2) ◽  
pp. 298-304 ◽  
Author(s):  
Ju¨rgen Lindner ◽  
Gerhard Zinser ◽  
William Werz ◽  
Christo Goridis ◽  
Bernard Bizzini ◽  
...  
Keyword(s):  
Cell Migration ◽  
Granule Cell ◽  
Cerebellar Granule Cell ◽  
Cerebellar Granule ◽  
Experimental Modification
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