Presence of different hepatitis C virus genotypes in plasma and peripheral blood mononuclear cell samples of Iranian patients with HIV infection

2018 ◽  
Vol 90 (8) ◽  
pp. 1343-1351 ◽  
Author(s):  
Farzaneh Dehghani-Dehej ◽  
Jamal Sarvari ◽  
Maryam Esghaei ◽  
Seyed Y. Hosseini ◽  
Saba Garshasbi ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood Mononuclear Cell ◽  
Hiv Infection ◽  
Mononuclear Cell ◽  
Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Virus Genotypes ◽  
Hepatitis C Virus Genotypes ◽  
Iranian Patients

scholarly journals Analysis of the binding of hepatitis C virus genotype 1a and 1b E2 glycoproteins to peripheral blood mononuclear cell subsets

2005 ◽  
Vol 86 (9) ◽  
pp. 2507-2512 ◽  
Author(s):  
Eriko Yamada ◽  
Maria Montoya ◽  
Christian G. Schuettler ◽  
Timothy P. Hickling ◽  
Alexander W. Tarr ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood Mononuclear Cell ◽  
Mononuclear Cell ◽  
Peripheral Blood ◽  
Type I ◽  
Cell Infection ◽  
Virus Genotype ◽  
Peripheral Blood Mononuclear ◽  
Genotype 1A

Hepatitis C virus (HCV) binding to hepatocytes is thought to be mediated via interaction of the E2 glycoprotein with (co-)receptors including CD81 and scavenger receptor class B type I (SR-BI). Here, the expression of CD81 and SR-BI was analysed on peripheral blood mononuclear cell (PBMC) subsets, and the binding of genotype 1 soluble truncated E2 (sE2) proteins to these cells was investigated. All PBMC subsets expressed CD81, although at varying levels. In contrast, SR-BI was only detected on monocytes and dendritic cells (DCs). The genotype 1a H77c sE2 protein showed higher PBMC binding than other genotype 1a/b sE2s. H77c sE2 binding to different PBMC subsets largely paralleled their level of CD81 expression, and could be inhibited by blocking E2–CD81 interaction. However, those PBMC subsets reported to be infected by HCV in vivo (monocytes, DCs and B cells) also exhibited residual, CD81-independent binding, indicating roles for SR-BI/other receptor(s) in mediating haematopoietic cell infection.

New evidence of replication of hepatitis C virus in short-term peripheral blood mononuclear cell cultures

2014 ◽  
Vol 191 ◽  
pp. 1-9 ◽  
Author(s):  
Federico Alejandro Di Lello ◽  
Andrés Carlos Alberto Culasso ◽  
Cecilia Parodi ◽  
Patricia Baré ◽  
Rodolfo Héctor Campos ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood Mononuclear Cell ◽  
Mononuclear Cell ◽  
Cell Cultures ◽  
Peripheral Blood ◽  
Short Term ◽  
Peripheral Blood Mononuclear ◽  
New Evidence

Peripheral blood mononuclear cell abnormalities and their relationship to clinical course in homosexual men with HIV infection

1988 ◽  
Vol 46 (2) ◽  
pp. 258-271 ◽  
Author(s):  
Renato M. de Martini ◽  
Roderick R. Turner ◽  
Silvia C. Formenti ◽  
Donna C. Boone ◽  
Philippe C. Bishop ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cell ◽  
Hiv Infection ◽  
Mononuclear Cell ◽  
Peripheral Blood ◽  
Clinical Course ◽  
Homosexual Men ◽  
Peripheral Blood Mononuclear

scholarly journals Compartmentalization of Hepatitis C Virus Genotypes between Plasma and Peripheral Blood Mononuclear Cells

2005 ◽  
Vol 79 (10) ◽  
pp. 6349-6357 ◽  
Author(s):  
Anne-Marie Roque-Afonso ◽  
Delphine Ducoulombier ◽  
Gaëtana Di Liberto ◽  
Rachid Kara ◽  
Michele Gigou ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Significant Proportion ◽  
Single Strand Conformation Polymorphism ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Virus Genotypes

ABSTRACT Differences in hepatitis C virus (HCV) variants of the highly conserved 5′ untranslated region (UTR) have been observed between plasma and peripheral blood mononuclear cells (PBMC). The prevalence and the mechanisms of this compartmentalization are unknown. Plasma and PBMC HCV variants were compared by single-strand conformation polymorphism (SSCP) and by cloning or by genotyping with a line probe assay (LiPA) in 116 chronically infected patients, including 44 liver transplant recipients. SSCP patterns differed between compartments in 43/109 analyzable patients (39%). Differences were significantly more frequent in patients with transplants (21/38 [55%] versus 22/71 [31%]; P < 0.01) and in those who acquired HCV through multiple transfusions before 1991 (15/20; 75%) or through drug injection (16/31; 52%) than in those infected through an unknown route (7/29; 24%) or through a single transfusion (5/29; 17%; P < 0.001). Cloning of the 5′ UTR, LiPA analysis, and nonstructural region 5B sequencing revealed different genotypes in the two compartments from 10 patients (9%). In nine patients, the genotype detected in PBMC was not detected in plasma and was weak or undetectable in the liver in three cases. This genotypic compartmentalization persisted for years in three patients and after liver transplantation in two. The present study shows that a significant proportion of HCV-infected subjects harbor in their PBMC highly divergent variants which were likely acquired through superinfections.

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