Valuable antibody detection method for classifying hepatitis E virus genotypes

2017 ◽  
Vol 90 (1) ◽  
pp. 142-147 ◽  
Author(s):  
Chenyan Zhao ◽  
Yansheng Geng ◽  
Weijing Huang ◽  
Hongxia Ma ◽  
Youchun Wang
Keyword(s):  
Hepatitis E Virus ◽  
Detection Method ◽  
Hepatitis E ◽  
Antibody Detection ◽  
Virus Genotypes

Hepatitis E virus genotypes 1 and 3 in wastewater samples in Tunisia

2014 ◽  
Vol 160 (1) ◽  
pp. 183-189 ◽  
Author(s):  
A. Béji-Hamza ◽  
M. Hassine-Zaafrane ◽  
H. Khélifi-Gharbi ◽  
S. Della Libera ◽  
M. Iaconelli ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genotypes ◽  
Wastewater Samples

scholarly journals Infectivity and pathogenicity of different hepatitis E virus genotypes/subtypes in rabbit model

2020 ◽  
Vol 9 (1) ◽  
pp. 2697-2705
Author(s):  
Shuangshuang Li ◽  
Qiyu He ◽  
Li Yan ◽  
Manyu Li ◽  
Zhaochao Liang ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Rabbit Model ◽  
Hepatitis E ◽  
Virus Genotypes

scholarly journals Interlaboratory Validation of a Detection Method for Hepatitis E Virus RNA in Pig Liver

2020 ◽  
Vol 8 (10) ◽  
pp. 1460
Author(s):  
Eva Trojnar ◽  
Matthias Contzen ◽  
Dominik Moor ◽  
Anja Carl ◽  
Sabine Burkhardt ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Detection Method ◽  
Collaborative Study ◽  
False Negative ◽  
Rna Extraction ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Liver Sample ◽  
Pig Liver ◽  
Pork Liver

Background: In the last years, the number of notified hepatitis E cases in humans has continuously increased in Europe. Foodborne infection with the zoonotic hepatitis E virus (HEV) genotype 3 is considered the major cause of this disease. Undercooked liver and raw sausages containing the liver of pigs and wild boar are at high risk of containing HEV. However, so far, no standardized method for the detection of HEV-RNA in pig liver is available. Methods: An international collaborative study on method reproducibility involving 11 laboratories was performed for an HEV-RNA detection method, which consists of steps of sample homogenization, RNA extraction and real-time RT-PCR detection, including a process control. Naturally contaminated pork liver samples containing two different amounts of HEV and a HEV-negative pork liver sample were tested by all laboratories using the method. Results: Valid results were retrieved from 10 laboratories. A specificity of 100% and a sensitivity of 79% were calculated for the method. False negative results were only retrieved from the sample containing very low HEV amounts near the detection limit. Conclusions: The results show that the method is highly specific, sufficiently sensitive and robust for use in different laboratories. The method can, therefore, be applied to routine food control as well as in monitoring studies.

Detection of hepatitis E virus genotypes 3 and 4 in donkeys in northern China

2019 ◽  
Vol 52 (3) ◽  
pp. 415-419 ◽  
Author(s):  
P. Rui ◽  
F. Zhao ◽  
S. Yan ◽  
C. Wang ◽  
Q. Fu ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Northern China ◽  
Hepatitis E ◽  
Virus Genotypes

Comparison of real-time fluorescent RT-PCR and conventional RT-PCR for the detection of hepatitis E virus genotypes prevalent in China

2007 ◽  
Vol 79 (12) ◽  
pp. 1966-1973 ◽  
Author(s):  
Chenyan Zhao ◽  
Zhuo Li ◽  
Baoshan Yan ◽  
Tim J. Harrison ◽  
Xinhui Guo ◽  
...  
Keyword(s):  
Real Time ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Rt Pcr ◽  
Virus Genotypes

Temporal evolution of the distribution of hepatitis E virus genotypes in Southwestern France

2015 ◽  
Vol 35 ◽  
pp. 50-55 ◽  
Author(s):  
Sebastien Lhomme ◽  
Florence Abravanel ◽  
Martine Dubois ◽  
Sabine Chapuy-Regaud ◽  
Karine Sandres-Saune ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Temporal Evolution ◽  
Hepatitis E ◽  
Virus Genotypes

scholarly journals Sequence Variation in Hepatitis E Virus Genotypes 3 and 4 from Swine Fecal Samples in Japan

2009 ◽  
Vol 3 (1) ◽  
pp. 68-75 ◽  
Author(s):  
Sompong Sapsutthipas ◽  
Takeru Urayama ◽  
Masanobu Yamate ◽  
Muneo Tsujikawa ◽  
Hiromi Nishigaki ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Sequence Variation ◽  
Hepatitis E ◽  
Fecal Samples ◽  
Virus Genotypes

scholarly journals Hepatitis E virus in humans, farm animals and animals from the sylvatic environment

2017 ◽  
Vol 73 (8) ◽  
pp. 456-461
Author(s):  
Ewelina Bigoraj ◽  
Artur Rzeżutka
Keyword(s):  
Hepatitis E Virus ◽  
Animal Species ◽  
Current Knowledge ◽  
Animal Health ◽  
Hepatitis E ◽  
Farm Animals ◽  
Animal Reservoir ◽  
Full Spectrum ◽  
Virus Genotypes ◽  
Public Health Protection

Hepatitis E virus (HEV) is a hepatovirus causing infections in humans and in many animal species. According to the current knowledge, HEV strains have been classified in the genus Orthohepevirus, family Hepeviridae, which encompasses strains belonging to one of seven virus genotypes. Genotypes 1 and 2 have only been found in humans, while genotypes 3 and 4 have been detected in humans, pigs, deer, rabbits and mongoose. The other HEV genotypes infect wild animals. However, the full spectrum of animal species being the natural reservoir of HEV has not been fully recognized. The clinical course of hepatitis in animals is asymptomatic, and infections do not cause significant losses in animal farming. Unlike in animals, infections in humans, and especially in pregnant women, can cause serious health problems. The identification of new virus strains in the animal reservoir and the possibility of transmission of some animal HEV strains to humans make the issue of public health protection and food safety even more important. This article provides an overview of data on the prevalence of HEV infections in animals and their impact on human and animal health

scholarly journals A Comparative Study of Assay Performance of Commercial Hepatitis E Virus Enzyme-Linked Immunosorbent Assay Kits in Australian Blood Donor Samples

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Ashish C. Shrestha ◽  
Robert L. P. Flower ◽  
Clive R. Seed ◽  
Susan L. Stramer ◽  
Helen M. Faddy
Keyword(s):  
Blood Donor ◽  
Disease Burden ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Poor Agreement ◽  
Igm Elisa ◽  
Assay Performance ◽  
Antibody Elisa

Hepatitis E virus (HEV) is transfusion-transmissible and therefore poses a risk to blood transfusion safety. Seroprevalence studies are useful for estimating disease burden and determining risk factors. Considerable variability in the sensitivity of HEV antibody detection assays exists. This study aimed to compare the performances of commercially available HEV enzyme-linked immunosorbent assays (ELISA) in Australian blood donor samples. Plasma samples that tested positive (n=194) or negative (n=200) for HEV IgG (Wantai HEV IgG ELISA) were selected. Of the 194 HEV IgG positive samples, 4 were positive for HEV IgM (Wantai HEV IgM ELISA). All samples were tested with the MP Diagnostics: HEV IgG ELISA, total (IgG, IgM, and IgA) HEV antibody ELISA, and HEV IgM ELISA. Of the 194 Wantai HEV IgG positive samples, 92 (47%) tested positive with the MP Diagnostics HEV IgG ELISA (κ=0.47) and 126 (65%) with MP Diagnostics total HEV antibody assay (κ=0.65). There was poor agreement between Wantai and MP Diagnostics HEV IgM assays. This study demonstrated poor agreement between the assays tested. These observations are consistent with previous reports demonstrating significant variability between HEV ELISAs, highlighting that results of HEV serology should be interpreted with caution.

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