Full-length genomic sequence analysis of new subtype 3k hepatitis E virus isolates with 99.97% nucleotide identity obtained from two consecutive acute hepatitis patients in a city in northeast Japan

2016 ◽  
Vol 89 (6) ◽  
pp. 1116-1120 ◽  
Author(s):  
Masahito Miura ◽  
Jun Inoue ◽  
Mio Tsuruoka ◽  
Tsutomu Nishizawa ◽  
Shigeo Nagashima ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Acute Hepatitis ◽  
Hepatitis E Virus ◽  
Genomic Sequence ◽  
Hepatitis E ◽  
Full Length ◽  
Nucleotide Identity ◽  
Genomic Sequence Analysis ◽  
Virus Isolates

Full genomic sequence analysis of swine genotype 3 hepatitis E virus isolated from Shanghai

2009 ◽  
Vol 144 (1-2) ◽  
pp. 290-293 ◽  
Author(s):  
Fu-sheng Si ◽  
Yu-min Zhu ◽  
Shi-juan Dong ◽  
Shui-sheng Yu ◽  
Rui-song Yu ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Hepatitis E Virus ◽  
Genomic Sequence ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Full Genomic Sequence ◽  
Genomic Sequence Analysis

Full-length sequence analysis of hepatitis E virus isolates: showing potential determinants of virus genotype and identity

Virus Genes ◽  
2013 ◽  
Vol 47 (3) ◽  
pp. 414-421 ◽  
Author(s):  
Dong Yang ◽  
Mei Jiang ◽  
Min Jin ◽  
Zhigang Qiu ◽  
Weihong Cui ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Full Length ◽  
Virus Genotype ◽  
Full Length Sequence ◽  
Virus Isolates

Rat Hepatitis E Virus Linked to Severe Acute Hepatitis in an Immunocompetent Patient

2019 ◽  
Vol 220 (6) ◽  
pp. 951-955 ◽  
Author(s):  
Anton Andonov ◽  
Mark Robbins ◽  
Jamie Borlang ◽  
Jingxin Cao ◽  
Todd Hatchette ◽  
...  
Keyword(s):  
Acute Hepatitis ◽  
Hepatitis E Virus ◽  
Genomic Sequence ◽  
Hepatitis E ◽  
The United States ◽  
Immunocompetent Patient ◽  
Health Concern ◽  
Animal Reservoir ◽  
Hepatitis Infection ◽  
Severe Acute Hepatitis

Abstract Hepatitis E virus (HEV) is a major public health concern in developing countries where the primary transmission is via contaminated water. Zoonotic HEV cases have been increasingly described in Europe, Japan, and the United States, with pigs representing the main animal reservoir of infection. We report an unusual acute hepatitis infection in a previously healthy man caused by a rat HEV with a considerably divergent genomic sequence compared with other rat HEV strains. It is possible that rat HEV is an underrecognized cause of hepatitis infection, and further studies are necessary to elucidate its potential risk and mode of transmission.

Analysis of the full-length genome of genotype 4 hepatitis E virus isolates from patients with fulminant or acute self-limited hepatitis E

2006 ◽  
Vol 78 (4) ◽  
pp. 476-484 ◽  
Author(s):  
Jun Inoue ◽  
Tsutomu Nishizawa ◽  
Masaharu Takahashi ◽  
Tatsuya Aikawa ◽  
Hitoshi Mizuo ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Full Length ◽  
Genotype 4 ◽  
Full Length Genome ◽  
Virus Isolates

scholarly journals Analysis of the full-length genome of hepatitis E virus isolates obtained from wild boars in Japan

2005 ◽  
Vol 86 (12) ◽  
pp. 3321-3326 ◽  
Author(s):  
Tsutomu Nishizawa ◽  
Masaharu Takahashi ◽  
Kazunori Endo ◽  
Shinji Fujiwara ◽  
Nobuo Sakuma ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Full Length ◽  
Partial Sequence ◽  
Genotype 3 ◽  
Entire Genome ◽  
Wild Boars ◽  
Full Length Sequence ◽  
Full Length Genome ◽  
Virus Isolates

Two (2·3 %) of 87 wild-caught boars in Japan had detectable hepatitis E virus (HEV) RNA. The two boar HEV isolates (wbJTS1 and wbJYG1) obtained in the present study and a previously reported isolate (wbJSG1) whose partial sequence had been determined were sequenced over the entire genome. The wbJSG1, wbJTS1 and wbJYG1 isolates comprised 7225 or 7226 nt, excluding the poly(A) tail, and segregated into genotype 3. They differed by 8·5–11·2 % from each other and by 8·6–18·4 % from 17 reported genotype 3 HEV isolates, including one boar isolate, in the full-length sequence. When compared with 191 reported genotype 3 HEV isolates whose partial sequences were known, these three boar isolates were closer to Japanese isolates than to isolates of non-Japanese origin (89·2±2·6 vs 85·9±2·2 %; P<0·0001). A proportion of wild boars in Japan are infected with markedly heterogeneous HEV strains that are indigenous to Japan and may serve as reservoirs of HEV.

Reevaluation of a North India isolate of hepatitis E virus based on the full-length genomic sequence obtained following long RT-PCR

2002 ◽  
Vol 86 (1-2) ◽  
pp. 53-58 ◽  
Author(s):  
Shahid Jameel ◽  
Mohammad Zafrullah ◽  
Yogesh K. Chawla ◽  
Jang B. Dilawari
Keyword(s):  
Hepatitis E Virus ◽  
Genomic Sequence ◽  
Hepatitis E ◽  
Full Length ◽  
North India ◽  
Rt Pcr

scholarly journals Recombinant Hepatitis E Viruses Harboring Tags in the ORF1 Protein

2019 ◽  
Vol 93 (19) ◽  
Author(s):  
Dagmara Szkolnicka ◽  
Angela Pollán ◽  
Nathalie Da Silva ◽  
Noémie Oechslin ◽  
Jérôme Gouttenoire ◽  
...  
Keyword(s):  
Life Cycle ◽  
Acute Hepatitis ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Viral Life Cycle ◽  
Full Length ◽  
Epitope Tag ◽  
Replication Complex ◽  
Orf1 Protein ◽  
Limited Availability

ABSTRACT Hepatitis E virus (HEV) is one of the most common causes of acute hepatitis and jaundice in the world. Current understanding of the molecular virology and pathogenesis of hepatitis E is incomplete, due particularly to the limited availability of functional tools. Here, we report the development of tagged HEV genomes as a novel tool to investigate the viral life cycle. A selectable subgenomic HEV replicon was subjected to random 15-nucleotide sequence insertion using transposon-based technology. Viable insertions in the open reading frame 1 (ORF1) protein were selected in a hepatoblastoma cell line. Functional insertion sites were identified downstream of the methyltransferase domain, in the hypervariable region (HVR), and between the helicase and RNA-dependent RNA polymerase domains. HEV genomes harboring a hemagglutinin (HA) epitope tag or a small luciferase (NanoLuc) in the HVR were found to be fully functional and to allow the production of infectious virus. NanoLuc allowed quantitative monitoring of HEV infection and replication by luciferase assay. The use of HA-tagged replicons and full-length genomes allowed localization of putative sites of HEV RNA replication by the simultaneous detection of viral RNA by fluorescence in situ hybridization and of ORF1 protein by immunofluorescence. Candidate HEV replication complexes were found in cytoplasmic dot-like structures which partially overlapped ORF2 and ORF3 proteins as well as exosomal markers. Hence, tagged HEV genomes yield new insights into the viral life cycle and should allow further investigation of the structure and composition of the viral replication complex. IMPORTANCE Hepatitis E virus (HEV) infection is an important cause of acute hepatitis and may lead to chronic infection in immunocompromised patients. Knowledge of the viral life cycle is incomplete due to the limited availability of functional tools. In particular, low levels of expression of the ORF1 protein or limited sensitivity of currently available antibodies or both limit our understanding of the viral replicase. Here, we report the successful establishment of subgenomic HEV replicons and full-length genomes harboring an epitope tag or a functional reporter in the ORF1 protein. These novel tools should allow further characterization of the HEV replication complex and to improve our understanding of the viral life cycle.

Full-Length Sequences of Two Hepatitis E Virus Isolates Representing an Eastern China-Indigenous Subgroup of Genotype 4

Intervirology ◽  
2007 ◽  
Vol 50 (3) ◽  
pp. 181-189 ◽  
Author(s):  
Masami Koike ◽  
Kazuaki Takahashi ◽  
Shunji Mishiro ◽  
Atsushi Matsui ◽  
Mie Inao ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Eastern China ◽  
Hepatitis E ◽  
Full Length ◽  
Genotype 4 ◽  
Virus Isolates

scholarly journals Complete Genome Sequences of Two Porcine Deltacoronavirus Strains from Henan Province, China

2019 ◽  
Vol 8 (10) ◽  
Author(s):  
Qingqing Liang ◽  
Bingxiao Li ◽  
Honglei Zhang ◽  
Hui Hu
Keyword(s):  
Sequence Analysis ◽  
Complete Genome ◽  
Genomic Sequence ◽  
Henan Province ◽  
Full Length ◽  
The Other ◽  
Genome Sequences ◽  
Fecal Samples ◽  
Genomic Sequence Analysis

In 2016 and 2018, two porcine deltacoronavirus (PDCoV) strains, CH-01 and HNZK-02, were identified from fecal samples of piglets with diarrhea in Henan Province, China. The full-length genomic sequence analysis indicated that these two strains had high nucleotide identities with the other Chinese PDCoV epidemic strains.

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