Molecular epidemiology of enteric viruses in patients with acute gastroenteritis in Aichi prefecture, Japan, 2008/09−2013/14

2015 ◽  
Vol 88 (7) ◽  
pp. 1180-1186 ◽  
Author(s):  
Noriko Nakamura ◽  
Shinichi Kobayashi ◽  
Hiroko Minagawa ◽  
Tadashi Matsushita ◽  
Wataru Sugiura ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Acute Gastroenteritis ◽  
Enteric Viruses ◽  
Aichi Prefecture

Molecular epidemiology and genetic diversity of norovirus infection in children with acute gastroenteritis in Bangladesh, 2014‐2019

2021 ◽  
Author(s):  
Shuvra Kanti Dey ◽  
Nadim Sharif ◽  
Baki Billah ◽  
Tanjir Tarek Ibn Siddique ◽  
Tarequl Islam ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Epidemiology ◽  
Acute Gastroenteritis

Molecular epidemiology of norovirus from patients with acute gastroenteritis in northwestern Spain

2014 ◽  
Vol 143 (2) ◽  
pp. 316-324 ◽  
Author(s):  
C. F. MANSO ◽  
J. L. ROMALDE
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Epidemiology ◽  
Genetic Drift ◽  
Acute Gastroenteritis ◽  
Age Groups ◽  
Capsid Gene ◽  
High Prevalence ◽  
High Incidence ◽  
Northwestern Spain

SUMMARYThe high incidence of norovirus (NoV) infections seems to be related to the emergence of new variants that evolved by genetic drift of the capsid gene. In this work, that represents a first effort to describe the molecular epidemiology of NoV in the northwest of Spain, a total of eight different NoV genotypes (GII.1, GII.3, GII.4, GII.6, GII.7, GII.12, GII.13, GII.14) were detected. The major genotypes observed were GII.4 (45·42%) and GII.14 (34·9%), being detected in all age groups. In addition, and although most of GII.4 sequences belonged to 2006b (7·2%) and 2010 (50·35%) variants, the presence of new NoV variants was observed. Phylogenetic analysis revealed that a high number of GII.4 sequences (35·24%) could be assigned to the newly emerging Sydney 2012 variant, even during late 2010. The high prevalence of NoV GII.14 observed in this study may indicate the emergence of this genotype in Spain.

Molecular epidemiology of noroviruses in children under 5 years of age with acute gastroenteritis in Yaoundé, Cameroon

2019 ◽  
Vol 91 (5) ◽  
pp. 738-743 ◽  
Author(s):  
Akongnwi E. Mugyia ◽  
Valentine N. Ndze ◽  
Jane-Francis T. K. Akoachere ◽  
Hannah Browne ◽  
Angeline Boula ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Acute Gastroenteritis ◽  
Children Under 5

Molecular epidemiology of human bocavirus infection in hospitalized children with acute gastroenteritis in South Africa, 2009‐2015

2019 ◽  
Vol 92 (8) ◽  
pp. 1124-1132 ◽  
Author(s):  
Rembuluwani Netshikweta ◽  
Lizyben Chidamba ◽  
Sandrama Nadan ◽  
Maureen B. Taylor ◽  
Nicola A. Page
Keyword(s):  
South Africa ◽  
Molecular Epidemiology ◽  
Acute Gastroenteritis ◽  
Human Bocavirus ◽  
Hospitalized Children

scholarly journals Recovirus NS1-2 Has Viroporin Activity That Induces Aberrant Cellular Calcium Signaling To Facilitate Virus Replication

mSphere ◽  
2019 ◽  
Vol 4 (5) ◽  
Author(s):  
Alicia C. Strtak ◽  
Jacob L. Perry ◽  
Mark N. Sharp ◽  
Alexandra L. Chang-Graham ◽  
Tibor Farkas ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Calcium Signaling ◽  
Virus Replication ◽  
Acute Gastroenteritis ◽  
Diarrheal Disease ◽  
Enteric Viruses ◽  
Functional Assay ◽  
Human Noroviruses ◽  
Tulane Virus ◽  
Enteric Caliciviruses

ABSTRACT Enteric viruses in the Caliciviridae family cause acute gastroenteritis in humans and animals, but the cellular processes needed for virus replication and disease remain unknown. A common strategy among enteric viruses, including rotaviruses and enteroviruses, is to encode a viral ion channel (i.e., viroporin) that is targeted to the endoplasmic reticulum (ER) and disrupts host calcium (Ca2+) homeostasis. Previous reports have demonstrated genetic and functional similarities between the nonstructural proteins of caliciviruses and enteroviruses, including the calicivirus NS1-2 protein and the 2B viroporin of enteroviruses. However, it is unknown whether caliciviruses alter Ca2+ homeostasis for virus replication or whether the NS1-2 protein has viroporin activity like its enterovirus counterpart. To address these questions, we used Tulane virus (TV), a rhesus enteric calicivirus, to examine Ca2+ signaling during infection and determine whether NS1-2 has viroporin activity that disrupts Ca2+ homeostasis. We found that TV increases Ca2+ signaling during infection and that increased cytoplasmic Ca2+ levels are important for efficient replication. Further, TV NS1-2 localizes to the endoplasmic reticulum, the predominant intracellular Ca2+ store, and the NS2 region has characteristics of a viroporin domain (VPD). NS1-2 had viroporin activity in a classic bacterial functional assay and caused aberrant Ca2+ signaling when expressed in mammalian cells, but truncation of the VPD abrogated these activities. Together, our data provide new mechanistic insights into the function of the NS2 region of NS1-2 and support the premise that enteric viruses, including those within Caliciviridae, exploit host Ca2+ signaling to facilitate their replication. IMPORTANCE Tulane virus is one of many enteric caliciviruses that cause acute gastroenteritis and diarrheal disease. Globally, enteric caliciviruses affect both humans and animals and amass >65 billion dollars per year in treatment and health care-associated costs, thus imposing an enormous economic burden. Recent progress has resulted in several cultivation systems (B cells, enteroids, and zebrafish larvae) to study human noroviruses, but mechanistic insights into the viral factors and host pathways important for enteric calicivirus replication and infection are still largely lacking. Here, we used Tulane virus, a calicivirus that is biologically similar to human noroviruses and can be cultivated by conventional cell culture, to identify and functionally validate NS1-2 as an enteric calicivirus viroporin. Viroporin-mediated calcium signaling may be a broadly utilized pathway for enteric virus replication, and its existence within caliciviruses provides a novel approach to developing antivirals and comprehensive therapeutics for enteric calicivirus diarrheal disease outbreaks.

scholarly journals Molecular epidemiology of norovirus associated with acute gastroenteritis in Taizhou, China: A retrospective study

2020 ◽  
Vol 13 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Weiwei Shen ◽  
Ying Sheng ◽  
Jian Weng ◽  
Guixia Li ◽  
Donghong Wang ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Molecular Epidemiology ◽  
Acute Gastroenteritis

Detection of enteric viruses in rectal swabs from children with acute gastroenteritis attending the pediatric outpatient clinics in Sapporo, Japan

2009 ◽  
Vol 46 (1) ◽  
pp. 94-97 ◽  
Author(s):  
Kaori Nakanishi ◽  
Takeshi Tsugawa ◽  
Shinjiro Honma ◽  
Shuji Nakata ◽  
Masatoshi Tatsumi ◽  
...  
Keyword(s):  
Acute Gastroenteritis ◽  
Enteric Viruses ◽  
Outpatient Clinics ◽  
Rectal Swabs

Molecular epidemiology of enteric viruses in children with sporadic gastroenteritis in Valencia, Venezuela

2011 ◽  
Vol 83 (11) ◽  
pp. 1972-1982 ◽  
Author(s):  
Germán G. González ◽  
Ferdinando Liprandi ◽  
Juan E. Ludert
Keyword(s):  
Molecular Epidemiology ◽  
Enteric Viruses

Prevalence and Molecular Epidemiology of Noroviruses in Hospitalized Children With Acute Gastroenteritis in Rio de Janeiro, Brazil, 2004

2007 ◽  
Vol 26 (7) ◽  
pp. 602-606 ◽  
Author(s):  
Mat??as Victoria ◽  
Filipe An??bal Carvalho-Costa ◽  
Marcos Bryan Heinemann ◽  
Jos?? Paulo Leite ◽  
Marize Miagostovich
Keyword(s):  
Molecular Epidemiology ◽  
Acute Gastroenteritis ◽  
Rio De Janeiro ◽  
Hospitalized Children

scholarly journals A large infantile gastroenteritis outbreak in Albania caused by multiple emerging rotavirus genotypes

2003 ◽  
Vol 131 (3) ◽  
pp. 1105-1110 ◽  
Author(s):  
C. VILLENA ◽  
R. GABRIELI ◽  
R. M. PINTÓ ◽  
S. GUIX ◽  
D. DONIA ◽  
...  
Keyword(s):  
Acute Gastroenteritis ◽  
Distribution System ◽  
Water Distribution ◽  
Water Distribution System ◽  
Age Groups ◽  
Enteric Viruses ◽  
Nucleic Acid Amplification ◽  
The Public ◽  
Serotype 1 ◽  
Public Health Institute

By the end of December 2000, the epidemiological system ‘Alert’ of the Public Health Institute in Tirane reported an outbreak of acute gastroenteritis. The outbreak involved children in Tirane and in the rural area. In total, 2722 children were seen in Tirane Hospital and 982 (56·4%) were treated for acute gastroenteritis. The age group with the highest morbidity was 0–5 years (89·7%), followed by the 6–9 (6·2%) and 10–15 years age groups (4·1%). The distribution of acute gastroenteritis cases, which occurred along the same water distribution system, suggests a waterborne origin. The nucleic acid amplification confirmed the co-circulation of different genotypes of rotavirus, mainly P[8]G9 and P[8]G3, responsible for the outbreak. Other enteric viruses such as astrovirus serotype 1, adenovirus and Norovirus, genogroups I and II were detected. Co-infections with different rotavirus genotypes and even with different enteric viruses were detected in several samples.

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