Molecular epidemiology of human bocavirus infection in hospitalized children with acute gastroenteritis in South Africa, 2009‐2015

2019 ◽  
Vol 92 (8) ◽  
pp. 1124-1132 ◽  
Author(s):  
Rembuluwani Netshikweta ◽  
Lizyben Chidamba ◽  
Sandrama Nadan ◽  
Maureen B. Taylor ◽  
Nicola A. Page
Keyword(s):  
South Africa ◽  
Molecular Epidemiology ◽  
Acute Gastroenteritis ◽  
Human Bocavirus ◽  
Hospitalized Children

Prevalence and Molecular Epidemiology of Noroviruses in Hospitalized Children With Acute Gastroenteritis in Rio de Janeiro, Brazil, 2004

2007 ◽  
Vol 26 (7) ◽  
pp. 602-606 ◽  
Author(s):  
Mat??as Victoria ◽  
Filipe An??bal Carvalho-Costa ◽  
Marcos Bryan Heinemann ◽  
Jos?? Paulo Leite ◽  
Marize Miagostovich
Keyword(s):  
Molecular Epidemiology ◽  
Acute Gastroenteritis ◽  
Rio De Janeiro ◽  
Hospitalized Children

scholarly journals Human Bocavirus Prevalence In Children With Acute Gastroenteritis From Rural Communities In The Northen Region Of South Africa

2019 ◽  
Author(s):  
Mpumelelo Casper Rikhotso ◽  
Ronewa Khumela ◽  
Jean Pierre Kabue ◽  
Afsatou Ndama Traoré ◽  
Natasha Potgieter
Keyword(s):  
South Africa ◽  
Rural Communities ◽  
Real Time ◽  
Real Time Pcr ◽  
Acute Gastroenteritis ◽  
Enteric Viruses ◽  
Health Care Facilities ◽  
Human Bocavirus ◽  
Stool Samples ◽  
Pcr Targeting

AbstractBACKGROUNDAcute gastroenteritis (AGE) is a leading cause of morbidity and mortality in young children worldwide. Human Bocavirus (HBoV) is an emerging virus globally associated with diarrhea. The aim of this study was to demonstrate the prevalence of HBoV genotypes in children (≤5 years) from rural communities in South Africa (SA) suffering from AGE.MATERIAL AND METHODA total of 141 fecal samples of children ≤5 years with acute gastroenteritis (AGE) were collected from rural Primary Health Care facilities in the Vhembe district of SA between June 2017 and July 2018. Clinical symptoms and demographic data were also recorded. A total of 102 (72%) were outpatients and 39 (28%) were hospitalized patients. Human Bocavirus (HBoV) genotypes were determined using Real-Time Multiplex PCR. DNA extracts of positive samples were confirmed by conventional PCR targeting the NS1 gene. Co-infection with other enteric viruses were determined in HBoV positive samples using Real-Time PCR.RESULTSHBoV was detected in 8 (5.7%) children with AGE. Children were in the age group between 1-24 months. HBoV1 and HBoV3 genotypes were each detected in 3 (37.5%) stool samples and HBoV2 in 2 (25%) stool samples. Co-infection with other enteric viruses included Rotavirus (37.5%); Adenovirus (37.5%); Norovirus (25%) and Astrovirus (12.5%).CONCLUSIONHBoV infections could be seen as a potential emerging diarrheal pathogen in South Africa. Further studies are required to understand the role of HBoV infections in children and adults with acute gastroenteritis.Author summaryAcute gastroenteritis (AGE) is recognized as a major cause for mortality in children ≤5 years of age in Africa and other developing countries. Viruses known to be involved in AGE includes Rotavirus, Norovirus, Astrovirus and Adenovirus and have been reported globally. Recently the Human Bocavirus (HBoV) have been reported in numerous studies globally as a potential cause of diarrhea. In this study, the prevalence and genetic diversity of human Bocavirus in children with AGE from rural communities in Limpopo, South Africa were investigated. In total, 141 stool samples from children ≤ 5 years with AGE were assessed for the presence of HBoV using Real-Time PCR. HBoV were detected in 8 (5.7%) patients and included 3 positive samples for HBoV1 and HBoV3 respectively and 2 positive for HBoV2. No HBoV4 were detected. Among the 8 positive HBoV samples, co-infection with other enteric viruses were found in 7 (87.5%) samples, while mono infection with HBoV alone was detected in 1 (12.5%) patient. HBoV mixed infection with Rotavirus (3/8; 37.5%); Adenovirus (3/8; 37.5%); Norovirus (2/8; 25%) and Astrovirus (1/8; 12.5%) were observed in this study. This study reported for the first time on the prevalence of human Bocavirus in children with AGE from rural communities in South Africa.

scholarly journals Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil

2019 ◽  
Vol 68 (8) ◽  
pp. 1233-1239 ◽  
Author(s):  
Luana S. Soares ◽  
Ana Beatriz F. Lima ◽  
Kamilla C. Pantoja ◽  
Patrícia S. Lobo ◽  
Jonas F. Cruz ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Acute Gastroenteritis ◽  
Human Bocavirus

Human bocavirus in hospitalized children with acute gastroenteritis in Russia from 2010 to 2012

2016 ◽  
Vol 37 ◽  
pp. 143-149 ◽  
Author(s):  
Alexander Tymentsev ◽  
Artem Tikunov ◽  
Elena Zhirakovskaia ◽  
Alexander Kurilschikov ◽  
Igor Babkin ◽  
...  
Keyword(s):  
Acute Gastroenteritis ◽  
Human Bocavirus ◽  
Hospitalized Children

Genotypes and molecular epidemiology of human astroviruses in hospitalized children with acute gastroenteritis in Rio de Janeiro, Brazil

2007 ◽  
Vol 79 (7) ◽  
pp. 939-944 ◽  
Author(s):  
Matías Victoria ◽  
Filipe Aníbal Carvalho-Costa ◽  
Marcos Bryan Heinemann ◽  
José Paulo Gagliardi Leite ◽  
Marize Pereira Miagostovich
Keyword(s):  
Molecular Epidemiology ◽  
Acute Gastroenteritis ◽  
Rio De Janeiro ◽  
Hospitalized Children ◽  
Human Astroviruses

scholarly journals Human Bocavirus in Hospitalized Children, South Africa

2006 ◽  
Vol 12 (7) ◽  
pp. 1457-1458 ◽  
Author(s):  
Heidi Smuts ◽  
Di Hardie
Keyword(s):  
South Africa ◽  
Human Bocavirus ◽  
Hospitalized Children

scholarly journals Predominance of Human Bocavirus Genotype 1 and 3 in Outpatient Children with Diarrhea from Rural Communities in South Africa, 2017–2018

Pathogens ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 245
Author(s):  
Mpumelelo Casper Rikhotso ◽  
Ronewa Khumela ◽  
Jean Pierre Kabue ◽  
Afsatou Ndama Traoré-Hoffman ◽  
Natasha Potgieter
Keyword(s):  
South Africa ◽  
Rural Communities ◽  
Real Time ◽  
Acute Gastroenteritis ◽  
Clinical Symptoms ◽  
Demographic Data ◽  
Enteric Viruses ◽  
Health Care Facilities ◽  
Human Bocavirus ◽  
Pcr Targeting

Human bocavirus (HBoV) is an emerging virus globally associated with diarrhea in young children. This study aims to investigate the prevalence of HBoV genotypes in children (≤5 years) from rural communities in South Africa (SA) suffering from acute gastroenteritis (AGE). A total of 141 fecal samples of children ≤5 years with acute gastroenteritis (AGE) were collected from rural primary health care facilities in the Vhembe district of SA between June 2017 and July 2018. Clinical symptoms and demographic data were also recorded. A total of 102 (72%) were outpatients, and 39 (28%) were hospitalized patients. Human bocavirus (HBoV) genotypes were determined using real-time multiplex PCR. DNA extracts of positive samples were confirmed by conventional PCR targeting the NS1 gene. Co-infection with other enteric viruses were determined in HBoV-positive samples using real-time PCR. HBoV was detected in eight (5.7%) children with AGE, of which three (37.5%) were HBoV1, three (37.5%) were HBoV3, and two (25%) were HBoV2. The majority of positive cases were identified in outpatients (62%) between the ages of 1 and 24 months. Co-infection in HBoV-positive samples with other enteric viruses included rotavirus (37.5%), adenovirus (37.5%), norovirus (25%), and astrovirus (12.5%). HBoV infections could be seen as a potential emerging diarrheal pathogen in South Africa. However, more studies are needed to understand the role of HBoV infections in children with AGE.

Molecular Epidemiology of Human Astrovirus Infection in Hospitalized Children with Acute Gastroenteritis

2006 ◽  
Vol 9 (2) ◽  
pp. 139 ◽  
Author(s):  
Ju Young Chung ◽  
Kyung Huh ◽  
Sang Woo Kim ◽  
Bo Mun Shin ◽  
Tae Hee Han ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Acute Gastroenteritis ◽  
Hospitalized Children ◽  
Human Astrovirus
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