Mass spectrometric and peptide chip characterization of an assembled epitope: analysis of a polyclonal antibody model serum directed against the Sjøgren/systemic lupus erythematosus autoantigen TRIM21

2013 ◽  
Vol 48 (6) ◽  
pp. 651-659 ◽  
Author(s):  
M. Al-Majdoub ◽  
C. Koy ◽  
P. Lorenz ◽  
H.-J. Thiesen ◽  
M. O. Glocker
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Polyclonal Antibody ◽  
Lupus Erythematosus ◽  
Mass Spectrometric ◽  
Systemic Lupus ◽  
Epitope Analysis ◽  
Peptide Chip

scholarly journals A Real-World Characterization of Patients With “Moderate-To-Severe” Systemic Lupus Erythematosus

2014 ◽  
Vol 17 (7) ◽  
pp. A522
Author(s):  
V. Strand ◽  
J. Johnson ◽  
C. Vandeloo ◽  
C. Galateanu ◽  
S. Lobosco
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Real World ◽  
Lupus Erythematosus ◽  
Systemic Lupus ◽  
Severe Systemic Lupus Erythematosus

MO430MASS-SPECTROMETRIC IDENTIFICATION OF POST-TRANSLATIONAL GUANIDINYLATED PROTEINS IN THE CONTEXT OF SYSTEMIC LUPUS ERYTHEMATOSUS

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Juliane Hermann ◽  
Ute Raffetseder ◽  
Michaela Lellig ◽  
Joachim Jankowski ◽  
Vera Jankowski
Keyword(s):  
Mass Spectrometry ◽  
Systemic Lupus Erythematosus ◽  
High Resolution ◽  
Lupus Erythematosus ◽  
Imaging Techniques ◽  
Mass Spectrometric ◽  
Mass Spectrometric Imaging ◽  
Systemic Lupus ◽  
Notch 3 ◽  
Resolution Mass

Abstract Background and Aims With continuous identification of post-translational modified isoforms of proteins, it is becoming increasingly clear that post-translational modifications limit or modify the biological functions of native proteins are majorly involved in development of various chronic disease. This is mostly due to technically advanced molecular identification and quantification methods, mainly based on mass spectrometry. Mass spectrometry has become one of the most powerful tools for the identification of lipids. Method In this study, we used sophisticated high-resolution mass-spectrometric methods to analyze the soluble ligand of receptor Notch-3, namely the Y-box protein (YB)-1, in serum from systemic lupus erythematosus (SLE) patients. In addition, kidneys of lupus-prone (MRL.lpr) mice were analyzed by mass-spectrometric imaging techniques to identify the underlying pathomechanisms. Serum YB-1 was isolated by chromatographic methods, afterwards digested by trypsin and analyzed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). The kidneys were fixed in paraffin, then kidney sections were deparaffinized, tryptic digested and analyzed by mass-spectrometric imaging techniques. Mass-spectrometry of extracellular YB-1 in SLE patient serum revealed post-translational guanidinylation of two lysine’s within the highly conserved cold shock domain (CSD) of the YB-1 protein (YB-1-2G). Patients with increased disease activity and those with active renal involvement (lupus nephritis, LN) had a higher degree of dual-guanidinylation within the CSD. Of note, at least one of these modifications was present in all analyzed LN patients, whereas single-guanidinylated YB-1 was present in only one and double modification in none of the control individuals. Mass-spectrometric imaging analyses specifically localized YB-1-2G and increases Notch-3 expression in kidney sections from MRL.lpr mice. Results The data from this study clearly demonstrate the high potential of high-resolution mass spectrometric methods as well as mass spectrometric imaging techniques to identify pathomechanisms of diseases like SLE/LN.

scholarly journals Frequency of SARS-CoV-2 infection and characterization of the clinical course in patients with Systemic Lupus Erythematosus in the city of Manaus-Amazonas

2021 ◽  
Author(s):  
Gabriella Bacellar Marques ◽  
Lucila Cristina Tomé Garcia ◽  
Cecília Tizatto Barroso ◽  
Vitória Miki Pang Takatani ◽  
Samuel Elias Basualto Dias ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Clinical Course ◽  
Systemic Lupus ◽  
The City

Characterization of C1q-binding IgG complexes in systemic lupus erythematosus

1984 ◽  
Vol 30 (1) ◽  
pp. 104-116 ◽  
Author(s):  
Shu Uwatoko ◽  
Shinichi Aotsuka ◽  
Masako Okawa ◽  
Yasuo Egusa ◽  
Ryuichi Yokohari ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Systemic Lupus

scholarly journals Molecular Characterization of Circulating Plasma Cells in Patients with Active Systemic Lupus Erythematosus

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e44362 ◽  
Author(s):  
Patricia L. Lugar ◽  
Cassandra Love ◽  
Amrie C. Grammer ◽  
Sandeep S. Dave ◽  
Peter E. Lipsky
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Molecular Characterization ◽  
Lupus Erythematosus ◽  
Plasma Cells ◽  
Active Systemic Lupus Erythematosus ◽  
Systemic Lupus
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