scholarly journals Future directions of structural mass spectrometry using hydroxyl radical footprinting

2010 ◽  
Vol 45 (12) ◽  
pp. 1373-1382 ◽  
Author(s):  
Janna G. Kiselar ◽  
Mark R. Chance
Keyword(s):  
Mass Spectrometry ◽  
Hydroxyl Radical ◽  
Future Directions ◽  
Structural Mass Spectrometry ◽  
Hydroxyl Radical Footprinting ◽  
Structural Mass

scholarly journals Hydroxyl radical footprinting analysis of a human haptoglobin-hemoglobin complex

2021 ◽  
Author(s):  
Dmitry S Loginov ◽  
Jan Fiala ◽  
Peter Brechlin ◽  
Gary Kruppa ◽  
Petr Novak
Keyword(s):  
Mass Spectrometry ◽  
Complex Formation ◽  
Photochemical Oxidation ◽  
Structure And Dynamics ◽  
Interaction Interface ◽  
Structural Mass Spectrometry ◽  
Hydroxyl Radical Footprinting ◽  
Structural Mass ◽  
Protein Structure And Dynamics

Methods of structural mass spectrometry have become more popular to study protein structure and dynamics. Among them, fast photochemical oxidation of proteins (FPOP) has several advantages such as irreversibility of modifications and more facile determination of the site of modification with single residue resolution. In the present study, FPOP analysis was applied to study the hemoglobin (Hb) – haptoglobin (Hp) complex allowing identification of respective regions altered upon the complex formation. Oxidative modifications were precisely localized on specific residues using a timsTOF Pro mass spectrometer. The data allowed determination of amino acids directly involved in Hb – Hp interactions and those located outside of the interaction interface yet affected by the complex formation. Data are available via ProteomeXchange with identifier PXD021621.

The interaction between the natural metalloendopeptidase inhibitor BJ46a and its target toxin jararhagin analyzed by structural mass spectrometry and molecular modeling

2020 ◽  
Vol 221 ◽  
pp. 103761
Author(s):  
Viviane A. Bastos ◽  
Francisco Gomes-Neto ◽  
Surza Lucia G. Rocha ◽  
André Teixeira-Ferreira ◽  
Jonas Perales ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Modeling ◽  
Structural Mass Spectrometry ◽  
Structural Mass

The growing role of structural mass spectrometry in the discovery and development of therapeutic antibodies

The Analyst ◽  
2018 ◽  
Vol 143 (11) ◽  
pp. 2459-2468 ◽  
Author(s):  
Yuwei Tian ◽  
Brandon T. Ruotolo
Keyword(s):  
Mass Spectrometry ◽  
State Of The Art ◽  
Therapeutic Antibodies ◽  
Critical Importance ◽  
Current State ◽  
Measurement Science ◽  
Structural Mass Spectrometry ◽  
Structural Mass

The comprehensive structural characterization of therapeutic antibodies is of critical importance for the successful discovery and development of such biopharmaceuticals, yet poses many challenges to modern measurement science. Here, we review the current state-of-the-art mass spectrometry technologies focusing on the characterization of antibody-based therapeutics.

scholarly journals Structural mass spectrometry of tissue extracts to distinguish cancerous and non-cancerous breast diseases

2014 ◽  
Vol 10 (11) ◽  
pp. 2827-2837 ◽  
Author(s):  
Kelly M. Hines ◽  
Billy R. Ballard ◽  
Dana R. Marshall ◽  
John A. McLean
Keyword(s):  
Mass Spectrometry ◽  
Breast Tissue ◽  
Breast Diseases ◽  
Molecular Signatures ◽  
Human Breast ◽  
Human Breast Tissue ◽  
Tissue Extracts ◽  
Ms Analysis ◽  
Structural Mass Spectrometry ◽  
Structural Mass

UPLC-IM-MS/MS analysis of human breast tissue extracts distinguished cancerous and non-cancerous breast diseases by characteristic molecular signatures.

scholarly journals Structural Mass Spectrometry: Rapid Methods for Separation and Analysis of Peptide Natural Products

2012 ◽  
Vol 75 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Cody R. Goodwin ◽  
Larissa S. Fenn ◽  
Dagmara K. Derewacz ◽  
Brian O. Bachmann ◽  
John A. McLean
Keyword(s):  
Mass Spectrometry ◽  
Natural Products ◽  
Rapid Methods ◽  
Structural Mass Spectrometry ◽  
Structural Mass
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