scholarly journals Study of laser ablation in the in vivo rabbit brain with MR thermometry

2002 ◽  
Vol 16 (2) ◽  
pp. 147-152 ◽  
Author(s):  
Lili Chen ◽  
Janaka P. Wansapura ◽  
Gary Heit ◽  
Kim Butts
Keyword(s):  
Laser Ablation ◽  
Rabbit Brain ◽  
Mr Thermometry

In vivo image-guided MR thermometry during laser ablation: experience in kidney and liver

2018 ◽  
Author(s):  
Paola Saccomandi ◽  
Celine Giraudau ◽  
Federico Davrieux ◽  
Giuseppe Quero ◽  
Emiliano Schena ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Mr Thermometry ◽  
Image Guided

scholarly journals Development of a photochemical thrombosis investigation system to obtain a rabbit ischemic stroke model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoonhee Kim ◽  
Yoon Bum Lee ◽  
Seung Kuk Bae ◽  
Sung Suk Oh ◽  
Jong-ryul Choi
Keyword(s):  
Ischemic Stroke ◽  
Brain Damage ◽  
Rabbit Model ◽  
Specific Area ◽  
Brain Atlas ◽  
Rabbit Brain ◽  
Stroke Model ◽  
Triphenyltetrazolium Chloride ◽  
Induction System

AbstractPhotochemical thrombosis is a method for the induction of ischemic stroke in the cerebral cortex. It can generate localized ischemic infarcts in the desired region; therefore, it has been actively employed in establishing an ischemic stroke animal model and in vivo assays of diagnostic and therapeutic techniques for stroke. To establish a rabbit ischemic stroke model and overcome the shortcoming of previous studies that were difficult to build a standardized photothrombotic rabbit model, we developed a photochemical thrombosis induction system that can produce consistent brain damage on a specific area. To verify the generation of photothrombotic brain damage using the system, longitudinal magnetic resonance imaging, 2,3,5-triphenyltetrazolium chloride staining, and histological staining were applied. These analytical methods have a high correlation for ischemic infarction and are appropriate for analyzing photothrombotic brain damage in the rabbit brain. The results indicated that the photothrombosis induction system has a main advantage of being accurately controlled a targeted region of photothrombosis and can produce cerebral hemisphere lesions on the target region of the rabbit brain. In conjugation with brain atlas, it can induce photochemical ischemic stroke locally in the part of the brain that is responsible for a particular brain function and the system can be used to develop animal models with degraded specific functions. Also, the photochemical thrombosis induction system and a standardized rabbit ischemic stroke model that uses this system have the potential to be used for verifications of biomedical techniques for ischemic stroke at a preclinical stage in parallel with further performance improvements.

scholarly journals Myelin replacement triggered by single-cell demyelination in mouse cortex

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Nicolas Snaidero ◽  
Martina Schifferer ◽  
Aleksandra Mezydlo ◽  
Bernard Zalc ◽  
Martin Kerschensteiner ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Single Cell ◽  
In Vivo Imaging ◽  
Active Participant ◽  
Cell Replacement ◽  
Myelin Sheaths ◽  
Unmyelinated Axons ◽  
Single Axon ◽  
Mouse Cortex

Abstract Myelin, rather than being a static insulator of axons, is emerging as an active participant in circuit plasticity. This requires precise regulation of oligodendrocyte numbers and myelination patterns. Here, by devising a laser ablation approach of single oligodendrocytes, followed by in vivo imaging and correlated ultrastructural reconstructions, we report that in mouse cortex demyelination as subtle as the loss of a single oligodendrocyte can trigger robust cell replacement and remyelination timed by myelin breakdown. This results in reliable reestablishment of the original myelin pattern along continuously myelinated axons, while in parallel, patchy isolated internodes emerge on previously unmyelinated axons. Therefore, in mammalian cortex, internodes along partially myelinated cortical axons are typically not reestablished, suggesting that the cues that guide patchy myelination are not preserved through cycles of de- and remyelination. In contrast, myelin sheaths forming continuous patterns show remarkable homeostatic resilience and remyelinate with single axon precision.

Fast PRF-based MR thermometry using double-echo EPI: in vivo comparison in a clinical hyperthermia setting

2014 ◽  
Vol 28 (4) ◽  
pp. 305-314 ◽  
Author(s):  
Tetiana Dadakova ◽  
Johanna Gellermann ◽  
Otilia Voigt ◽  
Jan Gerrit Korvink ◽  
John Matthew Pavlina ◽  
...  
Keyword(s):  
Mr Thermometry

Improved MR Thermometry to Measure Brain Temperatures

2008 ◽  
Author(s):  
Devashish Shrivastava ◽  
Lance DelaBarre ◽  
Timothy Hanson ◽  
J. Thomas Vaughan
Keyword(s):  
High Field ◽  
Core Body Temperature ◽  
Temperature Response ◽  
Time History ◽  
Rf Heating ◽  
Mr Thermometry ◽  
Ultra High Field ◽  
High Fields ◽  
Rf Safety

An MR thermometry technique with sub-degree celsius accuracy is needed to measure in vivo temperatures vs. time in porcine brains at ultra-high fields. Porcine models are used to study thermoregulatory temperature response of the ultra-high field radiofrequency (RF) heating. The porcine hot critical temperature limit is comparable to and lower than that of humans. Also, porcine thermoregulatory mechanisms are similar to humans. Thus, conservative porcine thermoregulatory temperature responses can help develop new RF safety thresholds for ultra-high field human MRI. Sub-degree C temperature accuracy is needed since RF safety guidelines limit the maximum in vivo head temperature change due to RF heating to 1 °C over the core body temperature. Three-dimensional temperature maps over time are required since non-uniform RF power deposition at ultra-high fields and blood flow produce non-uniform in vivo temperatures with local hot spots. Thermogenic hazards are related to in vivo temperatures and temperature-time history — and not to the typically measured whole head average specific absorption rate.

In Vivo MR Thermometry of Frozen Tissue Using R2* and Signal Intensity1

2005 ◽  
Vol 12 (9) ◽  
pp. 1080-1084 ◽  
Author(s):  
Janaka P. Wansapura ◽  
Bruce L. Daniel ◽  
Karl K. Vigen ◽  
Kim Butts
Keyword(s):  
Mr Thermometry ◽  
Frozen Tissue
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