Studies of the interactions between the anticytokeratin 8 monoclonal antibody TS1, its antigen and its anti-idiotypic antibody αTS1

2003 ◽  
Vol 16 (3) ◽  
pp. 157-163 ◽  
Author(s):  
Ann Erlandsson ◽  
Patrik Holm ◽  
Amanda Ullén ◽  
Torgny Stigbrand ◽  
Birgitta E. Sundström
Keyword(s):  
Monoclonal Antibody ◽  
Idiotypic Antibody

Anti-idiotypic antibodies bear the internal image of a human tumor antigen

Science ◽  
1986 ◽  
Vol 232 (4746) ◽  
pp. 100-102 ◽  
Author(s):  
D Herlyn ◽  
AH Ross ◽  
H Koprowski
Keyword(s):  
Monoclonal Antibody ◽  
Immune Response ◽  
Gastric Carcinoma ◽  
Tumor Antigen ◽  
Human Tumor ◽  
Internal Image ◽  
Tumor Associated Antigen ◽  
Idiotypic Antibody ◽  
Mouse Igg2a ◽  
Igg1 Isotype

Goat antibodies to idiotypes (anti-idiotypic antibodies; Ab2) that recognize an idiotype associated with the combining site of a BALB/c mouse IgG2a monoclonal antibody (Ab1) to human gastric carcinoma were used to immunize BALB/c mice and rabbits. A monoclonal anti-anti-idiotypic antibody (Ab3) of IgG1 isotype was obtained after immunization of mice. The Ab3 and the Ab1 showed identical binding specificities and bound with similar avidities to the same tumor antigen. The induction of Ab1-like Ab3 by Ab2 was not restricted to mice, since Ab3 could also be induced in rabbits. Both the mouse- and the rabbit-derived Ab3 bound the same gastrointestinal cancer-associated antigen as Ab1. These findings indicate that Ab2 induced the formation of antigen-specific Ab3, probably because it bears the internal image of the tumor-associated antigen. This Ab2 may therefore have potential for modulating the immune response of cancer patients to their tumors.

Anti-idiotypic antibody Ab2/3H6 mimics the epitope of the neutralizing anti-HIV-1 monoclonal antibody 2F5

AIDS ◽  
2002 ◽  
Vol 16 (4) ◽  
pp. 667-668 ◽  
Author(s):  
Renate E. Kunert ◽  
Robert Weik ◽  
Boris Ferko ◽  
Gabriela Stiegler ◽  
Hermann Katinger
Keyword(s):  
Monoclonal Antibody ◽  
Idiotypic Antibody ◽  
Anti Hiv ◽  
Hiv 1

Idiometric assay: noncompetitive immunoassay for small molecules typified by the measurement of estradiol in serum

1990 ◽  
Vol 36 (11) ◽  
pp. 1945-1950 ◽  
Author(s):  
G Barnard ◽  
F Kohen
Keyword(s):  
Monoclonal Antibody ◽  
Small Molecules ◽  
Molecular Mass ◽  
Binding Site ◽  
Hypervariable Region ◽  
Primary Antibody ◽  
Good Sensitivity ◽  
Time Resolved ◽  
Excess Amount ◽  
Idiotypic Antibody

Abstract We report here a novel noncompetitive immunoassay applicable to the measurement of small-molecular-mass compounds and typified by the direct measurement of estradiol in serum. Two types of anti-idiotypic antibody recognize different epitopes within the hypervariable region of the specific primary antibody (e.g., anti-estradiol). The first anti-idiotype (betatype) recognizes an epitope at the unoccupied binding site, which is masked in the presence of the analyte (e.g., estradiol). The second (alphatype) recognizes an epitope close to the binding site and is unaffected by the presence or absence of the analyte, but is sterically hindered from binding to the primary antibody in the presence of the betatype. The use of these matched antibodies (primary antibody, alphatype, and betatype) has enabled the development of a method for determining antibody occupancy that is not based on a conventional two-site assay. An excess amount of purified monoclonal antibody against estradiol is immobilized onto the walls of microtiter wells. After the capture of analyte, the unoccupied antibody sites are blocked by the addition of an excess amount of betatype. Subsequently, analyte occupancy is determined by the addition of excess europium-labeled alphatype, incubation, washing, and time-resolved fluorometry. The method demonstrates good sensitivity, precision, and comparability with alternative competitive immunoassays.

Idiotype vaccine for tumor by anti-idiotypic antibody prepared against anti-(bacillus Calmette Guèrin)BCG monoclonal antibody

1990 ◽  
Vol 31 (5) ◽  
pp. 273-277 ◽  
Author(s):  
Jin-ichi Sasaki ◽  
Takuma Tamagake ◽  
Satoshi Narita ◽  
Chowdhury Rafiqul Ahsan
Keyword(s):  
Monoclonal Antibody ◽  
Bacillus Calmette Guerin ◽  
Idiotype Vaccine ◽  
Idiotypic Antibody
Sign in / Sign up

Export Citation Format

Share Document