Molecular recognition between oligopeptides and nucleic acids. Sequence specific binding of (4S)-(+)- and (4R)-(−)-dihydrokikumycin B to DNA deduced form1H NMR, footprinting studies and thermodynamic data

1989 ◽  
Vol 2 (1) ◽  
pp. 6-17 ◽  
Author(s):  
Moses Lee ◽  
Regan G. Shea ◽  
John A. Hartley ◽  
J. Willliam Lown ◽  
Koren Kissinger ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Nucleic Acids ◽  
Thermodynamic Data ◽  
Specific Binding

scholarly journals On the formation of dendrimer/nucleolipids surface films for directed self-assembly

Soft Matter ◽  
2015 ◽  
Vol 11 (10) ◽  
pp. 1973-1990 ◽  
Author(s):  
Marianna Yanez Arteta ◽  
Debora Berti ◽  
Costanza Montis ◽  
Richard A. Campbell ◽  
Caroline Eriksson ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Nucleic Acids ◽  
Self Assembly ◽  
Surface Films

Molecular recognition of nucleic acids by dendrimers with non-covalently attached nucleolipids can be tuned by nucleolipid type and order of addition.

Molecular recognition between oligopeptides and nucleic acids. DNA sequence specificity and binding properties of an acridine-linked netropsin hybrid ligand

1990 ◽  
Vol 3 (1) ◽  
pp. 26-35 ◽  
Author(s):  
Christian Bailly ◽  
Nicole Helbecque ◽  
Jean-Pierre Hénichart ◽  
Pierre Colson ◽  
Claude Houssier ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Nucleic Acids ◽  
Dna Sequence ◽  
Sequence Specificity ◽  
Binding Properties ◽  
Dna Sequence Specificity

scholarly journals Recombinase-Based Isothermal Amplification of Nucleic Acids with Self-Avoiding Molecular Recognition Systems (SAMRS)

ChemBioChem ◽  
2014 ◽  
Vol 15 (15) ◽  
pp. 2268-2274 ◽  
Author(s):  
Nidhi Sharma ◽  
Shuichi Hoshika ◽  
Daniel Hutter ◽  
Kevin M. Bradley ◽  
Steven A. Benner
Keyword(s):  
Molecular Recognition ◽  
Nucleic Acids ◽  
Isothermal Amplification ◽  
Recognition Systems

scholarly journals Structural basis of anti-SARS-CoV-2 activity of HCQ: specific binding to N protein to disrupt its interaction with nucleic acids and LLPS

2021 ◽  
Author(s):  
Jianxing Song ◽  
Mei Dang
Keyword(s):  
Life Cycle ◽  
Nucleic Acids ◽  
Large Scale ◽  
Specific Binding ◽  
Viral Life Cycle ◽  
Structural Basis ◽  
N Protein ◽  
Binding Residues ◽  
Almost All ◽  
Key Steps

Abstract Great efforts have led to successfully developing the spike-based vaccines but challenges still exist to completely terminate the SARS-CoV-2 pandemic. SARS-CoV-2 nucleocapsid (N) protein plays the essential roles in almost all key steps of the viral life cycle, thus representing a top drug target. Almost all key functions of N protein including liquid-liquid phase separation (LLPS) depend on its capacity in interacting with nucleic acids. Therefore, only the variants with their N proteins functional in binding nucleic acids might survive and spread in evolution and indeed, the residues critical for binding nucleic acids are highly conserved. Very recently, hydroxychloroquine (HCQ) was shown to prevent the transmission in a large-scale clinical study in Singapore but so far, no specific SARS-CoV-2 protein was experimentally identified to be targeted by HCQ. Here by NMR, we unambiguously decode that HCQ specifically binds NTD and CTD of SARS- CoV-2 N protein with Kd of 112.1 and 57.1 μM respectively to inhibit their interaction with nucleic acid, as well as to disrupt LLPS essential for the viral life cycle. Most importantly, HCQ-binding residues are identical in SARS-CoV-2 variants and therefore HCQ is likely effective to them all. The results not only provide a structural basis for the anti-SARS-CoV-2 activity of HCQ, but also renders HCQ to be the first known drug capable of targeting LLPS. Furthermore, the unique structure of the HCQ-CTD complex decodes a promising strategy for further design of better anti-SARS-CoV-2 drugs from HCQ. Therefore, HCQ is a promising candidate to help terminate the pandemic.

scholarly journals Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications

2015 ◽  
Vol 2015 ◽  
pp. 1-31 ◽  
Author(s):  
Ka Lok Hong ◽  
Letha J. Sooter
Keyword(s):  
Nucleic Acid ◽  
Molecular Recognition ◽  
Nucleic Acids ◽  
Antibody Fragments ◽  
Small Peptides ◽  
Basic Principles ◽  
Single Stranded Dna ◽  
High Affinity ◽  
Ssdna Aptamer ◽  
Recognition Elements

Molecular recognition elements (MREs) can be short sequences of single-stranded DNA, RNA, small peptides, or antibody fragments. They can bind to user-defined targets with high affinity and specificity. There has been an increasing interest in the identification and application of nucleic acid molecular recognition elements, commonly known as aptamers, since they were first described in 1990 by the Gold and Szostak laboratories. A large number of target specific nucleic acids MREs and their applications are currently in the literature. This review first describes the general methodologies used in identifying single-stranded DNA (ssDNA) aptamers. It then summarizes advancements in the identification and biosensing application of ssDNA aptamers specific for bacteria, viruses, their associated molecules, and selected chemical toxins. Lastly, an overview of the basic principles of ssDNA aptamer-based biosensors is discussed.

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